Am J Physiol Cell Physiol AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 273: C456-C472, 1997;
0363-6143/97 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kenyon, E.
Right arrow Articles by Miller, S. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kenyon, E.
Right arrow Articles by Miller, S. S.

AJP - Cell Physiology, Vol 273, Issue 2 C456-C472, Copyright © 1997 by American Physiological Society

ARTICLES

Apical and basolateral membrane mechanisms that regulate pHi in bovine retinal pigment epithelium

E. Kenyon, A. Maminishkis, D. P. Joseph and S. S. Miller
School of Optometry, University of California, Berkeley 94720, USA.

pH regulation was studied in fresh explant bovine retinal pigment epithelium-choroid using the pH-sensitive dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein and intracellular microelectrodes. Acid recovery was HCO3 dependent, inhibited by apical amiloride and apical or basal 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), and required apical and basal Na. Alkali recovery was HCO3 dependent and inhibitable by apical or basal DIDS. Three apical and two basolateral transporters were identified. Four contribute to acid extrusion, i.e., apical Na/H exchange, apical H-lactate cotransport, and apical Na-HCO3 cotransport and basolateral Na-HCO3 cotransport. At least two contribute to alkali extrusion, i.e., apical Na-HCO3 cotransport and a basolateral HCO3-dependent, DIDS-inhibitable mechanism, possibly Na-HCO3 cotransport, Cl/HCO3 exchange, or both. The apical Na-HCO3 cotransporter is electrogenic, carrying net negative charge inward. Basal Cl removal or addition of basal HCO3 caused HCO3- and Cl-dependent alkalinizations, respectively. Apical DIDS increased both responses. These cytosolic pH (pHi) regulatory mechanisms are so tightly coupled that changes in pHi can only occur after two or more of them are inhibited. In addition, these mechanisms help provide pathways for transport of Na and HCO3 across the retinal pigment epithelium between the blood and the distal retina.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
L. L. Daniele, B. Sauer, S. M. Gallagher, E. N. Pugh Jr, and N. J. Philp
Altered visual function in monocarboxylate transporter 3 (Slc16a8) knockout mice
Am J Physiol Cell Physiol, August 1, 2008; 295(2): C451 - C457.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Z. Qu and H. C. Hartzell
Bestrophin Cl- channels are highly permeable to HCO3-
Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1371 - C1377.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. A. Hughes and A. Swaminathan
Modulation of the Kir7.1 potassium channel by extracellular and intracellular pH
Am J Physiol Cell Physiol, February 1, 2008; 294(2): C423 - C431.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
O. Strauss
The Retinal Pigment Epithelium in Visual Function
Physiol Rev, July 1, 2005; 85(3): 845 - 881.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Petrovic, S. Barone, A. M. Weinstein, and M. Soleimani
Activation of the apical Na+/H+ exchanger NHE3 by formate: a basis of enhanced fluid and electrolyte reabsorption by formate in the kidney
Am J Physiol Renal Physiol, August 1, 2004; 287(2): F336 - F346.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
N. J. Philp, D. Wang, H. Yoon, and L. M. Hjelmeland
Polarized Expression of Monocarboxylate Transporters in Human Retinal Pigment Epithelium and ARPE-19 Cells
Invest. Ophthalmol. Vis. Sci., April 1, 2003; 44(4): 1716 - 1721.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. Maminishkis, S. Jalickee, S. A. Blaug, J. Rymer, B. R. Yerxa, W. M. Peterson, and S. S. Miller
The P2Y2 Receptor Agonist INS37217 Stimulates RPE Fluid Transport In Vitro and Retinal Reattachment in Rat
Invest. Ophthalmol. Vis. Sci., November 1, 2002; 43(11): 3555 - 3566.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Bok, M. J. Schibler, A. Pushkin, P. Sassani, N. Abuladze, Z. Naser, and I. Kurtz
Immunolocalization of electrogenic sodium-bicarbonate cotransporters pNBC1 and kNBC1 in the rat eye
Am J Physiol Renal Physiol, November 1, 2001; 281(5): F920 - F935.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. Rymer, S. S. Miller, and J. L. Edelman
Epinephrine-Induced Increases in [Ca2+]in and KCl-Coupled Fluid Absorption in Bovine RPE
Invest. Ophthalmol. Vis. Sci., July 1, 2001; 42(8): 1921 - 1929.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. M. Sciortino and M. F. Romero
Cation and voltage dependence of rat kidney electrogenic Na+-HCO-3 cotransporter, rkNBC, expressed in oocytes
Am J Physiol Renal Physiol, October 1, 1999; 277(4): F611 - F623.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
N. J. Philp, H. Yoon, and E. F. Grollman
Monocarboxylate transporter MCT1 is located in the apical membrane and MCT3 in the basal membrane of rat RPE
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 1998; 274(6): R1824 - R1828.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online