Am J Physiol Cell Physiol AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 273: C404-C419, 1997;
0363-6143/97 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gibb, C. A.
Right arrow Articles by Cook, D. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gibb, C. A.
Right arrow Articles by Cook, D. I.

AJP - Cell Physiology, Vol 273, Issue 2 C404-C419, Copyright © 1997 by American Physiological Society

ARTICLES

Control of cytosolic pH in two-cell mouse embryos: roles of H(+)-lactate cotransport and Na+/H+ exchange

C. A. Gibb, P. Poronnik, M. L. Day and D. I. Cook
Department of Physiology, University of Sydney, New South Wales, Australia.

In this study we used imaging techniques with the fluorescent pH-sensitive dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein to investigate the control of cytosolic pH (pHi) in two-cell mouse embryos in nominally HCO3(-)-free conditions. We found that the resting pHi of two-cell embryos (40-50 h after human chorionic gonadotropin) in HCO3(-)-free M2 was 7.31 +/- 0.01 (n = 172 embryos), which is significantly above the level predicted if H+ is at electrochemical equilibrium. We showed that two-cell embryos contain a H(+)-monocarboxylate cotransport system with apparent Michaelis constants for D-lactate, L-lactate, and pyruvate of 11.5, 3.7, and 3.5 mM, respectively. It is inhibited by p-chloromercuribenzoic acid (300 microM), p-chloromercuriphenylsulfonic acid (300 microM), and alpha-cyano-4-hydroxycinnamate (1 mM) and is insensitive to 4,4'-diisothiocyanodihydrostilbene-2,2'-disulfonic acid (500 microM). We also showed that the pHi response to the acid load produced by an NH4Cl pulse has two components, one due to H(+)-monocarboxylate cotransport and the other due to Na+/H+ exchange. We found no evidence that a H+ conductance was responsible in these cells for the recovery in pHi after an acid load.


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
E.A. Harding, C.A. Gibb, M.H. Johnson, D.I. Cook, and M.L. Day
Developmental Changes in the Management of Acid Loads During Preimplantation Mouse Development
Biol Reprod, November 1, 2002; 67(5): 1419 - 1429.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. L. Steeves, M. Lane, B. D. Bavister, K. P. Phillips, and J. M. Baltz
Differences in Intracellular pH Regulation by Na+/H+ Antiporter among Two-Cell Mouse Embryos Derived from Females of Different Strains
Biol Reprod, July 1, 2001; 65(1): 14 - 22.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
K. P. Phillips, M.-C. Leveille, P. Claman, and J. M. Baltz
Intracellular pH regulation in human preimplantation embryos
Hum. Reprod., April 1, 2000; 15(4): 896 - 904.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online