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Am J Physiol Cell Physiol 272: C1373-C1379, 1997;
0363-6143/97 $5.00
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AJP - Cell Physiology, Vol 272, Issue 4 C1373-C1379, Copyright © 1997 by American Physiological Society

ARTICLES

Modes of operation of an electroneutral Na+/Li+ countertransport in human skin fibroblasts

G. Zerbini, R. Mangili, D. Gabellini and G. Pozza
Division of Medicine, Scientific Institute San Raffaele, University of Milan, Italy.

An elevated activity of erythrocyte Na+/Li+ countertransport (SLC) is an intermediate phenotype of human essential hypertension, but cells other than erythrocytes have not been studied. Therefore, we have examined several transport modes of Na+/Li+ exchange in human skin fibroblasts. External Na+-stimulated Li+ efflux was 152 +/- 31 (SE) nmol x mg protein(-1) x min(-1) (n = 8). At intracellular pH 7.3, intracellular Na+-stimulated Li+ influx, intracellular Li+-stimulated Na+ influx, and external Li+-stimulated Na+ efflux were very similar, indicating the presence of a tightly coupled 1:1 SLC. This pathway was not affected by 5-(N,N-dimethyl)-amiloride and changes in the membrane potential, but phloretin and intracellular acidification (intracellular pH 6.8) were markedly inhibitory. Kinetic analyses of the external Na+ site also compared with SLC, although the internal site appeared to show a low affinity for Li+. We conclude that an SLC pathway similar to that in human erythrocytes is expressed in human skin fibroblasts.


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M. Cirillo, M. Laurenzi, W. Panarelli, M. Trevisan, and J. Stamler
Prospective Analysis of Traits Related to 6-Year Change in Sodium-Lithium Countertransport
Hypertension, March 1, 1999; 33(3): 887 - 893.
[Abstract] [Full Text] [PDF]


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