Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 272: C1169-C1177, 1997;
0363-6143/97 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jin, W.
Right arrow Articles by Hopfer, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jin, W.
Right arrow Articles by Hopfer, U.

AJP - Cell Physiology, Vol 272, Issue 4 C1169-C1177, Copyright © 1997 by American Physiological Society

ARTICLES

Purinergic-mediated inhibition of Na+-K+-ATPase in proximal tubule cells: elevated cytosolic Ca2+ is not required

W. Jin and U. Hopfer
Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4970, USA.

The involvement of cytosolic Ca2+ concentration ([Ca2+]i) as messenger for the regulation of Na+-K+-ATPase activity was investigated in a renal cell line recently developed by immortalization of early proximal tubule primary cultures from the Wistar-Kyoto rat strain. Na+-K+-ATPase was measured as short-circuit current (Isc) in intact monolayers after permeabilization of the apical plasma membrane with amphotericin B. With symmetrical solutions, Isc quantitatively reflects Na+-K+-ATPase activity as judged by ouabain inhibition and dependence on Na+ and K+. Extracellular ATP (50% effective concentration = 0.32 mM) on the apical side produced acute inhibition of Na+-K+-ATPase-generated Isc of up to 50%. The inhibition peaked within 1 min and lasted approximately 5 min. The potency order was ATP > ADP >> beta,gamma-methyleneadenosine 5'-triphosphate = UTP, consistent with a P2y receptor. Extracellular ATP also stimulated a transient increase in [Ca2+]i. This increase had a similar time course as the inhibition of ATPase and reached a peak change of approximately 120 nM. However, the elevation of [Ca2+]i is not required in the purinergic inhibition of the Na+-K+-ATPase, since, first, increases in [Ca2+]i produced with a Ca2+ ionophore (ionomycin) failed to mimic the purinergic inhibition and, second, 1,2-bis(2-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid, which abolished the [Ca2+]i elevation, failed to block the purinergic inhibition.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
G. B. Silva and J. L. Garvin
Extracellular ATP inhibits transport in medullary thick ascending limbs: role of P2X receptors
Am J Physiol Renal Physiol, November 1, 2009; 297(5): F1168 - F1173.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. M. Vekaria, D. G. Shirley, J. Sevigny, and R. J. Unwin
Immunolocalization of ectonucleotidases along the rat nephron
Am J Physiol Renal Physiol, February 1, 2006; 290(2): F550 - F560.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. J. Lee, S. H. Park, and H. J. Han
ATP stimulates Na+-glucose cotransporter activity via cAMP and p38 MAPK in renal proximal tubule cells
Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1268 - C1276.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Yamamoto and Y. Suzuki
Role of luminal ATP in regulating electrogenic Na+ absorption in guinea pig distal colon
Am J Physiol Gastrointest Liver Physiol, August 1, 2002; 283(2): G300 - G308.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. W. Inscho
P2 receptors in regulation of renal microvascular function
Am J Physiol Renal Physiol, June 1, 2001; 280(6): F927 - F944.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. M. Schwiebert and B. K. Kishore
Extracellular nucleotide signaling along the renal epithelium
Am J Physiol Renal Physiol, June 1, 2001; 280(6): F945 - F963.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
X.-L. Cui, W.-W. Jin, Y.-X. Ding, L. D. Alexander, U. Hopfer, and J. G. Douglas
Ca2+-dependent activation of c-jun NH2-terminal kinase in primary rabbit proximal tubule epithelial cells
Am J Physiol Cell Physiol, August 1, 2000; 279(2): C403 - C409.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. E. McCoy, A. L. Taylor, B. A. Kudlow, K. Karlson, M. J. Slattery, L. M. Schwiebert, E. M. Schwiebert, and B. A. Stanton
Nucleotides regulate NaCl transport in mIMCD-K2 cells via P2X and P2Y purinergic receptors
Am J Physiol Renal Physiol, October 1, 1999; 277(4): F552 - F559.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Zhang, C. E. Magyar, J. M. Norian, N.-H. Holstein-Rathlou, A. K. Mircheff, and A. A. McDonough
Reversible effects of acute hypertension on proximal tubule sodium transporters
Am J Physiol Cell Physiol, April 1, 1998; 274(4): C1090 - C1100.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W. Jin and U. Hopfer
Dipeptide-induced Cl- secretion in proximal tubule cells
Am J Physiol Cell Physiol, November 1, 1997; 273(5): C1623 - C1631.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online