Am J Physiol Cell Physiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 272: C1160-C1168, 1997;
0363-6143/97 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (29)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ho, M. W.
Right arrow Articles by French, A. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ho, M. W.
Right arrow Articles by French, A. S.

AJP - Cell Physiology, Vol 272, Issue 4 C1160-C1168, Copyright © 1997 by American Physiological Society

ARTICLES

Ins(3,4,5,6)P4 specifically inhibits a receptor-mediated Ca2+-dependent Cl- current in CFPAC-1 cells

M. W. Ho, S. B. Shears, K. S. Bruzik, M. Duszyk and A. S. French
Department of Physiology, University of Alberta, Edmonton, Canada.

We have examined the role of inositol 3,4,5,6-tetrakisphosphate [Ins(3,4,5,6)P4] in the control of Cl- current in CFPAC-1 cells. Intracellular Ins(3,4,5,6)P4 had no effect on basal current, but it produced a five- to sevenfold reduction in the Cl- current stimulated by either 2 microM extracellular ATP or by 1 microM extracellular thapsigargin. The half-maximally effective dose of Ins(3,4,5,6)P4 was 2.9 microM, and 4 microM blocked >80% of the ATP-activated current. In contrast, 10 microM Ins(1,4,5,6)P4, Ins(1,3,4,5)P4, or Ins(1,3,4,6)P4 enhanced rather than inhibited the ATP-activated Cl- current, although Ins(1,4,5,6)P4 only acted transiently. These stimulatory effects were Ca2+ dependent and largely inhibited by coapplication of equimolar Ins(3,4,5,6)P4. Inositol 1,3,4,5,6-pentakisphosphate, the precursor of Ins(3,4,5,6)P4, did not affect Cl- current. These data consolidate and extend the hypothesis that Ins(3,4,5,6)P4 is an important intracellular regulator of Cl- current in epithelial cells.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Moody, C. Pennington, C. Schultz, R. Caldwell, C. Dinkel, M. W. Rossi, S. McNamara, J. Widdicombe, S. Gabriel, and A. E. Traynor-Kaplan
Inositol polyphosphate derivative inhibits Na+ transport and improves fluid dynamics in cystic fibrosis airway epithelia
Am J Physiol Cell Physiol, September 1, 2005; 289(3): C512 - C520.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. E. Smitham and K. E. Barrett
Differential effects of apical and basolateral uridine triphosphate on intestinal epithelial chloride secretion
Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1431 - C1439.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. L. Clarke, M. C. Harline, M. A. Otero, G. G. Glover, R. C. Garrad, B. Krugh, N. M. Walker, F. A. Gonzalez, J. T. Turner, and G. A. Weisman
Desensitization of P2Y2 receptor-activated transepithelial anion secretion
Am J Physiol Cell Physiol, April 1, 1999; 276(4): C777 - C787.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Saiardi, J. J. Caffrey, S. H. Snyder, and S. B. Shears
The Inositol Hexakisphosphate Kinase Family. CATALYTIC FLEXIBILITY AND FUNCTION IN YEAST VACUOLE BIOGENESIS
J. Biol. Chem., August 4, 2000; 275(32): 24686 - 24692.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. W. Y. Ho, M. A. Kaetzel, D. L. Armstrong, and S. B. Shears
Regulation of a Human Chloride Channel. A PARADIGM FOR INTEGRATING INPUT FROM CALCIUM, TYPE II CALMODULIN-DEPENDENT PROTEIN KINASE, AND INOSITOL 3,4,5,6-TETRAKISPHOSPHATE
J. Biol. Chem., May 25, 2001; 276(22): 18673 - 18680.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online