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Am J Physiol Cell Physiol 272: C1099-C1111, 1997;
0363-6143/97 $5.00
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AJP - Cell Physiology, Vol 272, Issue 4 C1099-C1111, Copyright © 1997 by American Physiological Society

ARTICLES

Defining the volume dependence of multiple K flux pathways of trout red blood cells

M. Berenbrink, Y. R. Weaver and A. R. Cossins
Integrative Physiology Research Group, School of Biological Science, University of Liverpool, United Kingdom.

The volume sensitivity of different K flux pathways has been determined in trout red blood cells subjected to volume perturbation. Gentle hyposmotic swelling induced a K influx in a Cl-containing saline but not in NO3- or methanesulfonate (MeSF)-containing salines, consistent with the activation of a Cl-dependent flux. Extreme hyposmotic swelling led to larger K fluxes in all salines but with reduced anion discrimination of the Cl-dependent flux. In contrast to these graded responses, isosmotic swelling using ammonium chloride or beta-adrenergic stimulation activated only Cl-dependent fluxes in an all-or-none fashion. The relationship between the hyposmotically and isosmotically induced pathways was studied by coactivation using either ammonium chloride or isoproterenol with anisosmotic treatment. Cells in ammonium chloride-containing hyposmotic salines showed no additive K flux over that induced by hyposmotic treatment alone, indicating that the isosmotically induced Cl-dependent flux was identical to the hyposmotically induced Cl-dependent flux. However, cells coactivated by hyposmotic and beta-adrenergic treatment showed a small Cl-dependent flux in addition to that induced by hyposmotic treatment alone. This small third component was unaffected by anisosmotic treatment. We conclude that the major Cl-dependent and Cl-independent K flux pathways are distinct and separate and that the former has an anion dependence that varies with cell volume and a volume sensitivity that varies with ionic strength.


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H. Guizouarn and R. Motais
Swelling activation of transport pathways in erythrocytes: effects of Cl-, ionic strength, and volume changes
Am J Physiol Cell Physiol, January 1, 1999; 276(1): C210 - C220.
[Abstract] [Full Text] [PDF]


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