Am J Physiol Cell Physiol AJP: Renal Physiology
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Am J Physiol Cell Physiol 272: C1087-C1098, 1997;
0363-6143/97 $5.00
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AJP - Cell Physiology, Vol 272, Issue 4 C1087-C1098, Copyright © 1997 by American Physiological Society

ARTICLES

Calcium removal kinetics of the sarcoplasmic reticulum ATPase in skeletal muscle

E. E. Burmeister Getz and S. L. Lehman
Department of Biochemistry and Biophysics, University of California, San Francisco 94143, USA.

The models of the sarcoplasmic reticulum (SR) Ca pump used to simulate Ca kinetics in muscle fibers are simple but inconsistent with data on Ca binding or steady-state uptake. We develop a model of the SR pump that is consistent with data on transient and steady-state Ca removal and has rate constants identified under near-physiological conditions. We also develop models of the other main Ca-binding proteins in skeletal muscle: troponin C and parvalbumin. These models are used to simulate Ca transients in cut fibers during and after depolarizing pulses. Simulations using the full SR pump model are contrasted with simulations using a Michaelis-Menten (MM) approximation to SR pump kinetics. The MM pump underestimates the amount of Ca released during depolarization, underestimates the initial rate of Ca binding by the pump, and overestimates the later rate of Ca pumping. These errors are due to fast initial binding by the SR pump, which is neglected in the MM approximation.


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J. A. L. Jeneson, H. V. Westerhoff, and M. J. Kushmerick
A metabolic control analysis of kinetic controls in ATP free energy metabolism in contracting skeletal muscle
Am J Physiol Cell Physiol, September 1, 2000; 279(3): C813 - C832.
[Abstract] [Full Text] [PDF]


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