AJP - Cell Physiology, Vol 272, Issue 4 C1069-C1076, Copyright © 1997 by American Physiological Society

ARTICLES

Bowditch lecture. Integrated regulation of intestinal epithelial transport: intercellular and intracellular pathways

K. E. Barrett
Department of Medicine, University of California, San Diego, School of Medicine, 92103, USA.

The intestinal epithelium is an important site of active solute transport processes. Such processes include the secretion of electrolytes into the lumen, predominantly chloride and bicarbonate. These secretory mechanisms subserve a variety of functions, both physiological and pathophysiological, including maintenance of the fluidity of intestinal contents and mucosal defense. Both chloride and bicarbonate secretion are the subject of integrated regulatory mechanisms at both the intercellular and intracellular levels. The goal of this article is to discuss data that exemplify these two levels of regulation, which have been the subject of research in my laboratory. It is likely that some of these principles are also broadly applicable to secretory epithelial cells outside of the intestinal tract, such as those in the airway. I also discuss the ways in which we believe these regulatory mechanisms are involved not only in intestinal physiology but also perhaps in the pathogenesis of specific disease states.

This article has been cited by other articles:

				H. Dong, A. Smith, M. Hovaida, and J. Y. Chow Role of Ca2+-activated K+ channels in duodenal mucosal ion transport and bicarbonate secretion Am J Physiol Gastrointest Liver Physiol, December 1, 2006; 291(6): G1120 - G1128. [Abstract] [Full Text] [PDF]

				S. Resta-Lenert, F. Truong, K. E. Barrett, and L. Eckmann Inhibition of epithelial chloride secretion by butyrate: role of reduced adenylyl cyclase expression and activity Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1837 - C1849. [Abstract] [Full Text] [PDF]

				J. E. Smitham and K. E. Barrett Differential effects of apical and basolateral uridine triphosphate on intestinal epithelial chloride secretion Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1431 - C1439. [Abstract] [Full Text] [PDF]

				J. Venkatasubramanian, N. Selvaraj, M. Carlos, S. Skaluba, M. M. Rasenick, and M. C. Rao Differences in Ca2+ signaling underlie age-specific effects of secretagogues on colonic Cl{-} transport Am J Physiol Cell Physiol, March 1, 2001; 280(3): C646 - C658. [Abstract] [Full Text] [PDF]

				M. E. S. Kahn, A. Senderowicz, E. A. Sausville, and K. E. Barrett Possible Mechanisms of Diarrheal Side Effects Associated with the Use of a Novel Chemotherapeutic Agent, Flavopiridol Clin. Cancer Res., February 1, 2001; 7(2): 343 - 349. [Abstract] [Full Text]

				J. L. Dickson, T. D. Conner, and T. W. Ecay Inhibition of phosphatidylinositol 3-kinase does not alter forskolin-stimulated Cl- secretion by T84 cells Am J Physiol Cell Physiol, May 1, 2000; 278(5): C865 - C872. [Abstract] [Full Text] [PDF]

				S. J. Keely and K. E. Barrett ErbB2 and ErbB3 Receptors Mediate Inhibition of Calcium-dependent Chloride Secretion in Colonic Epithelial Cells J. Biol. Chem., November 19, 1999; 274(47): 33449 - 33454. [Abstract] [Full Text] [PDF]

				M. G. Lee, J. Y. Choi, X. Luo, E. Strickland, P. J. Thomas, and S. Muallem Cystic Fibrosis Transmembrane Conductance Regulator Regulates Luminal Cl-/HCO3- Exchange in Mouse Submandibular and Pancreatic Ducts J. Biol. Chem., May 21, 1999; 274(21): 14670 - 14677. [Abstract] [Full Text] [PDF]

				S. J. Keely, J. M. Uribe, and K. E. Barrett Carbachol Stimulates Transactivation of Epidermal Growth Factor Receptor and Mitogen-activated Protein Kinase in T84 Cells. IMPLICATIONS FOR CARBACHOL-STIMULATED CHLORIDE SECRETION J. Biol. Chem., October 16, 1998; 273(42): 27111 - 27117. [Abstract] [Full Text] [PDF]

				D. Merlin, L. Jiang, G. R. Strohmeier, A. Nusrat, S. L. Alper, W. I. Lencer, and J. L. Madara Distinct Ca2+- and cAMP-dependent anion conductances in the apical membrane of polarized T84 cells Am J Physiol Cell Physiol, August 1, 1998; 275(2): C484 - C495. [Abstract] [Full Text] [PDF]

				M. Donowitz, S. Khurana, C. M. Tse, and C. H. C. Yun III. Asymmetry in plasma membrane signal transduction: lessons from brush-border Na+/H+ exchangers Am J Physiol Gastrointest Liver Physiol, June 1, 1998; 274(6): G971 - G977. [Abstract] [Full Text] [PDF]

				K. E. Barrett, J. Smitham, A. Traynor-Kaplan, and J. M. Uribe Inhibition of Ca2+-dependent Cl- secretion in T84 cells: membrane target(s) of inhibition is agonist specific Am J Physiol Cell Physiol, April 1, 1998; 274(4): C958 - C965. [Abstract] [Full Text] [PDF]

				L. Eckmann, M. T. Rudolf, A. Ptasznik, C. Schultz, T. Jiang, N. Wolfson, R. Tsien, J. Fierer, S. B. Shears, M. F. Kagnoff, et al. D-myo-Inositol 1,4,5,6-tetrakisphosphate produced in human intestinal epithelial cells in response to Salmonella invasion inhibits phosphoinositide 3-kinase signaling pathways PNAS, December 23, 1997; 94(26): 14456 - 14460. [Abstract] [Full Text] [PDF]

				M. A. Carew, X. Yang, C. Schultz, and S. B. Shears myo-Inositol 3,4,5,6-Tetrakisphosphate Inhibits an Apical Calcium-activated Chloride Conductance in Polarized Monolayers of a Cystic Fibrosis Cell Line J. Biol. Chem., August 25, 2000; 275(35): 26906 - 26913. [Abstract] [Full Text] [PDF]