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Am J Physiol Cell Physiol 271: C1872-C1878, 1996;
0363-6143/96 $5.00
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AJP - Cell Physiology, Vol 271, Issue 6 C1872-C1878, Copyright © 1996 by American Physiological Society

ARTICLES

Sensitivity of volume-sensitive Cl- conductance in human epithelial cells to extracellular nucleotides

T. Tsumura, S. Oiki, S. Ueda, M. Okuma and Y. Okada
Department of Cellular and Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan.

The sensitivity to extracellular nucleotides of volume-sensitive Cl- channel activity was investigated by whole cell and single-channel patch-clamp recordings in a human small intestinal epithelial cell line (Intestine 407) during steady osmotic swelling. Adenine nucleotides added to the bathing solution suppressed whole cell volume-sensitive Cl- currents with the potency sequence of ATP > ADP > AMP. In contrast, extracellular adenosine 3',5'-cyclic monophosphate (cAMP) at over 0.1 mM increased volume-sensitive Cl- currents in the entire voltage range examined, whereas guanosine 3',5'-cyclic monophosphate was without effect. Neither the single-channel conductance nor the open probability was affected by extracellular cAMP. Extracellular ATP (at over 30 microM), in the Mg(2+)-free form, inhibited the whole cell volume-sensitive Cl- current, preferentially in the outward direction. By exposure to extracellular ATP, the single Cl- channel current became flickery at positive potentials. These results indicate that the volume-sensitive Cl- channel in the human epithelial cell is stimulated voltage independently by extracellular cAMP but blocked voltage dependently by the Mg(2+)-free form of extracellular ATP.


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