Am J Physiol Cell Physiol AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 271: C1539-C1545, 1996;
0363-6143/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (49)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qin, N.
Right arrow Articles by Stefani, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qin, N.
Right arrow Articles by Stefani, E.

AJP - Cell Physiology, Vol 271, Issue 5 C1539-C1545, Copyright © 1996 by American Physiological Society

ARTICLES

Identification of a second region of the beta-subunit involved in regulation of calcium channel inactivation

N. Qin, R. Olcese, J. Zhou, O. A. Cabello, L. Birnbaumer and E. Stefani
Department of Anesthesiology, University of California, Los Angeles 90095, USA.

Previous studies have shown that NH2 termini of the type 1 and 2 beta-subunits modulate the rate at which the neuronal alpha 1E calcium channel inactivates in response to voltage and that they do so independently of their common effect to stimulate activation by voltage (R. Olcese, N. Qin, T. Schneider, A. Neely, X. Wei, E. Stefani, and L. Birnbaumer, Neuron 13: 1433-1438, 1994). By constructing NH2-terminal deletions of several splice variants of beta-subunits, we have now found differences in the way they affect the rate of alpha 1E inactivation that lead us to identify a second domain that also regulates the rate of voltage-induced inactivation of the Ca2+ channel. This second domain, named segment 3, lies between two regions of high-sequence identity between all known beta-subunits and exists in two lengths (long and short), each encoded in a separate exon. Beta-Subunits with the longer 45- to 53-amino acid version cause the channel to inactivate more slowly than subunits with the shorter 7-amino acid version. As is the case for the NH2 terminus, the segment 3 does not affect the regulation of channel activation by the beta-subunit. In addition, the effect of the NH2-terminal segment prevails over that of the internal segment. This raises the possibility that phosphorylation, other types of posttranslational modification, or interaction with other auxiliary calcium channel subunits may be necessary to unmask the regulatory effect of the internal segment.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
V. Gudzenko, Y. Shiferaw, N. Savalli, R. Vyas, J. N. Weiss, and R. Olcese
Influence of channel subunit composition on L-type Ca2+ current kinetics and cardiac wave stability
Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1805 - H1815.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. D. Foell, R. C. Balijepalli, B. P. Delisle, A. M. R. Yunker, S. L. Robia, J. W. Walker, M. W. McEnery, C. T. January, and T. J. Kamp
Molecular heterogeneity of calcium channel {beta}-subunits in canine and human heart: evidence for differential subcellular localization
Physiol Genomics, April 13, 2004; 17(2): 183 - 200.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Geib, G. Sandoz, V. Cornet, K. Mabrouk, O. Fund-Saunier, D. Bichet, M. Villaz, T. Hoshi, J.-M. Sabatier, and M. De Waard
The Interaction between the I-II Loop and the III-IV Loop of Cav2.1 Contributes to Voltage-dependent Inactivation in a beta -Dependent Manner
J. Biol. Chem., March 15, 2002; 277(12): 10003 - 10013.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. D. Helton and W. A. Horne
Alternative Splicing of the beta 4 Subunit Has alpha 1 Subunit Subtype-Specific Effects on Ca2+ Channel Gating
J. Neurosci., March 1, 2002; 22(5): 1573 - 1582.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. A. Simen and R. J. Miller
Involvement of Regions in Domain I in the Opioid Receptor Sensitivity of alpha 1B Ca2+ Channels
Mol. Pharmacol., May 1, 2000; 57(5): 1064 - 1074.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
T. Cens, S. Restituito, S. Galas, and P. Charnet
Voltage and Calcium Use the Same Molecular Determinants to Inactivate Calcium Channels
J. Biol. Chem., February 26, 1999; 274(9): 5483 - 5490.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. Qin, R. Olcese, E. Stefani, and L. Birnbaumer
Modulation of human neuronal alpha 1E-type calcium channel by alpha 2delta -subunit
Am J Physiol Cell Physiol, May 1, 1998; 274(5): C1324 - C1331.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Qin, D. Platano, R. Olcese, J. L. Costantin, E. Stefani, and L. Birnbaumer
Unique regulatory properties of the type 2a Ca2+ channel beta  subunit caused by palmitoylation
PNAS, April 14, 1998; 95(8): 4690 - 4695.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Neuhuber, U. Gerster, J. Mitterdorfer, H. Glossmann, and B. E. Flucher
Differential Effects of Ca2+ Channel beta 1a and beta 2a Subunits on Complex Formation with alpha 1S and on Current Expression in tsA201 Cells
J. Biol. Chem., April 10, 1998; 273(15): 9110 - 9118.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Jiang, K. Spicher, G. Boulay, Y. Wang, and L. Birnbaumer
Most central nervous system D2 dopamine receptors are coupled to their effectors by Go
PNAS, March 13, 2001; 98(6): 3577 - 3582.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online