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Am J Physiol Cell Physiol 271: C804-C809, 1996;
0363-6143/96 $5.00
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AJP - Cell Physiology, Vol 271, Issue 3 C804-C809, Copyright © 1996 by American Physiological Society

ARTICLES

Impairment of insulin secretion in pancreatic islets isolated from Walker 256 tumor-bearing rats

S. el Razi Neto, T. M. Zorn, R. Curi and A. R. Carpinelli
Department of Physiology and Biophysics, University of Sao Paulo, Brazil.

Previous study has shown that insulin secretion in response to a glucose stimulus (16.7 mM) is reduced in islets isolated from Walker 256 tumor-bearing rats compared with controls. The ultrastructure, 45Ca2+ and 86Rb+ fractional outflow rate, phosphoinositide hydrolysis, and [U-14C]glucose decarboxylation were examined in islets isolated from tumor-bearing and control rats. The general morphological features of the islets from the control and experimental groups were very similar. The 86Rb+ fractional outflow rate was not changed, whereas the 45Ca2+ fractional outflow rate, [U-14C]glucose decarboxylation, and phosphoinositide metabolism were markedly reduced in islets from tumor-bearing rats. The changes in 45Ca2+ fractional outflow rate in islets from tumor-bearing rats were not due to impaired functioning of voltage-dependent calcium channels. By perifusing the islets in the presence of high potassium concentration, evidence was obtained that phospholipase C from islets from tumor-bearing rats reduced response to calcium. To further examine the mechanism involved in the impairment of insulin secretion by islets from tumor-bearing rats, islets isolated from normal rats were perifused after preincubation in the presence of serum from tumor-bearing rats. The results suggest that a thermolabile circulating factor is partially responsible for the changes described in islets isolated from Walker 256 tumor-bearing rats.


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H. R. Oliveira, R. Curi, and A. R. Carpinelli
Glucose induces an acute increase of superoxide dismutase activity in incubated rat pancreatic islets
Am J Physiol Cell Physiol, February 1, 1999; 276(2): C507 - C510.
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