AJP - Cell Physiology, Vol 271, Issue 3 C753-C762, Copyright © 1996 by American Physiological Society

ARTICLES

Cellular localization and regulation of CHIF in kidney and colon

C. Capurro, N. Coutry, J. P. Bonvalet, B. Escoubet, H. Garty and N. Farman
Institut National de la Sante et de la Recherche Medicale U. 246, Paris, France.

Channel inducing factor (CHIF) is a novel cDNA recently cloned from a rat distal colon cDNA library of dexamethasone-treated animals. While its expression in Xenopus oocytes evokes a potassium channel activity similar to that induced by Isk (minK), its cellular role is not clear. CHIF exhibits significant homologies with proteins that are putatively regulatory (phospholemman, gamma-subunit of Na(+)-K(+)-ATPase, Mat-8) while it differs from the small-conductance potassium channel Isk. We have studied the tissue specificity of CHIF expression in rat by in situ hybridization. CHIF is selectively present in the distal parts of the nephron (medullary and papillary collecting ducts and end portions of cortical collecting tubule) and in the epithelial cells of the distal colon. No expression of CHIF was found in renal proximal tubule, loop of Henle and distal tubule, proximal colon, small intestine, lung, choroid plexus, salivary glands, or brain. To gain some insight into CHIF function, we have investigated, using in situ hybridization and ribonuclease protection assay, whether CHIF mRNA expression could be altered in some situations. In the distal colon, corticosteroid hormones, sodium restriction, low-potassium diet, and metabolic acidosis significantly increased CHIF mRNA expression. In the kidney, metabolic acidosis was the only condition that showed an increase in CHIF mRNA expression. Some of these treatments also altered the expression of the colonic H(+)-K(+)-ATPase mRNA. In summary, CHIF mRNA is selectively expressed in the medullary collecting duct of the kidney and in the epithelium of the distal colon; its expression varies differently in these two target tissues after alterations in corticosteroid status, potassium depletion, and metabolic acidosis. The precise cell-specific functions of CHIF remain to be established.

This article has been cited by other articles:

				C. K. Tipsmark Identification of FXYD protein genes in a teleost: tissue-specific expression and response to salinity change Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2008; 294(4): R1367 - R1378. [Abstract] [Full Text] [PDF]

				M. Nowik, M. R. Lecca, A. Velic, H. Rehrauer, A. W. Brandli, and C. A. Wagner Genome-wide gene expression profiling reveals renal genes regulated during metabolic acidosis Physiol Genomics, February 19, 2008; 32(3): 322 - 334. [Abstract] [Full Text] [PDF]

				E. Arystarkhova, C. Donnet, A. Munoz-Matta, S. C. Specht, and K. J. Sweadner Multiplicity of expression of FXYD proteins in mammalian cells: dynamic exchange of phospholemman and {gamma}-subunit in response to stress Am J Physiol Cell Physiol, March 1, 2007; 292(3): C1179 - C1191. [Abstract] [Full Text] [PDF]

				K. Pihakaski-Maunsbach, H. Vorum, B. Honore, S. Tokonabe, J. Frokiaer, H. Garty, S. J. D. Karlish, and A. B. Maunsbach Locations, abundances, and possible functions of FXYD ion transport regulators in rat renal medulla Am J Physiol Renal Physiol, November 1, 2006; 291(5): F1033 - F1044. [Abstract] [Full Text] [PDF]

				Q. Cai, M. Keck, M. R. McReynolds, J. D. Klein, K. Greer, K. Sharma, J. B. Hoying, J. M. Sands, and H. L. Brooks Effects of water restriction on gene expression in mouse renal medulla: identification of 3betaHSD4 as a collecting duct protein Am J Physiol Renal Physiol, July 1, 2006; 291(1): F218 - F224. [Abstract] [Full Text] [PDF]

				K. L. Lansbery, L. C. Burcea, M. L. Mendenhall, and R. W. Mercer Cytoplasmic targeting signals mediate delivery of phospholemman to the plasma membrane Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1275 - C1286. [Abstract] [Full Text] [PDF]

				K. Geering FXYD proteins: new regulators of Na-K-ATPase Am J Physiol Renal Physiol, February 1, 2006; 290(2): F241 - F250. [Abstract] [Full Text] [PDF]

				H. Garty, M. Lindzen, R. Scanzano, R. Aizman, M. Fuzesi, R. Goldshleger, N. Farman, R. Blostein, and S. J. D. Karlish A functional interaction between CHIF and Na-K-ATPase: implication for regulation by FXYD proteins Am J Physiol Renal Physiol, October 1, 2002; 283(4): F607 - F615. [Abstract] [Full Text] [PDF]

				R. Aizman, C. Asher, M. Fuzesi, H. Latter, P. Lonai, S. J. D. Karlish, and H. Garty Generation and phenotypic analysis of CHIF knockout mice Am J Physiol Renal Physiol, September 1, 2002; 283(3): F569 - F577. [Abstract] [Full Text] [PDF]

				H. X. Pu, R. Scanzano, and R. Blostein Distinct Regulatory Effects of the Na,K-ATPase gamma Subunit J. Biol. Chem., May 31, 2002; 277(23): 20270 - 20276. [Abstract] [Full Text] [PDF]

				J. D. Stockand New ideas about aldosterone signaling in epithelia Am J Physiol Renal Physiol, April 1, 2002; 282(4): F559 - F576. [Abstract] [Full Text] [PDF]

				R. E. Booth, J. P. Johnson, and J. D. Stockand ALDOSTERONE Advan Physiol Educ, March 1, 2002; 26(1): 8 - 20. [Abstract] [Full Text] [PDF]

				H. Shi, R. Levy-Holzman, F. Cluzeaud, N. Farman, and H. Garty Membrane topology and immunolocalization of CHIF in kidney and intestine Am J Physiol Renal Physiol, March 1, 2001; 280(3): F505 - F512. [Abstract] [Full Text] [PDF]

				A. Naray-Fejes-Toth, C. Canessa, E. S. Cleaveland, G. Aldrich, and G. Fejes-Toth sgk Is an Aldosterone-induced Kinase in the Renal Collecting Duct. EFFECTS ON EPITHELIAL Na+ CHANNELS J. Biol. Chem., June 11, 1999; 274(24): 16973 - 16978. [Abstract] [Full Text] [PDF]

				F. Jaisser and A. T. Beggah The nongastric H+-K+-ATPases: molecular and functional properties Am J Physiol Renal Physiol, June 1, 1999; 276(6): F812 - F824. [Abstract] [Full Text] [PDF]

				C. YEAMAN, K. K. GRINDSTAFF, and W. J. NELSON New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity Physiol Rev, January 1, 1999; 79(1): 73 - 98. [Abstract] [Full Text] [PDF]

				H. X. Pu, F. Cluzeaud, R. Goldshleger, S. J. D. Karlish, N. Farman, and R. Blostein Functional Role and Immunocytochemical Localization of the gamma a and gamma b Forms of the Na,K-ATPase gamma Subunit J. Biol. Chem., June 1, 2001; 276(23): 20370 - 20378. [Abstract] [Full Text] [PDF]