| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 271, Issue 2 C666-C675, Copyright © 1996 by American Physiological Society
ARTICLES |
J. J. Widrick, S. W. Trappe, C. A. Blaser, D. L. Costill and R. H. Fitts
Department of Biology, Marquette University, Milwaukee, Wisconsin 53201, USA.
Single chemically permeabilized gastrocnemius fibers from six elite endurance-trained master runners (RUN group) and five age-matched sedentary controls (SED group) were mounted between a force transducer and a position motor, studied under conditions of maximal and submaximal Ca2+ activation, and subsequently electrophoresed on 5% polyacrylamide gels to determine myosin heavy chain (MHC) composition. For the SED group, peak isometric tension (Pzero) averaged 143 +/- 3, 156 +/- 4, and 170 +/- 4 kN/m2 and maximal shortening velocity (Vzero) averaged 0.43 +/- 0.01, 1.90 +/- 0.08, and 5.59 +/- 0.40 fiber lengths/s for fibers expressing type I, IIa, and IIx MHC, respectively (all comparisons, P < 0.05). Hill plot analysis of relative forces during submaximal Ca2+ activation indicated no SED vs. RUN differences in Ca2+ sensitivity or in the cooperativity of Ca2+ activation. However, at maximal Ca2+ activation, RUN type I and IIa fibers produced 15% less peak absolute force than SED fibers (P < 0.05). This reduction in fiber force was a direct result of the smaller diameter of the RUN fibers (P < 0.05), because Pzero, peak elastic modulus (Ezero), and Pzero/Ezero were not different between SED and RUN groups. RUN type I fibers also displayed a mean Vzero that was 19% higher than the average Vzero of the SED type I fibers (P < 0.05). In separate experiments, quantification of relative myosin light chain (MLC) isoform content revealed a 28% greater ratio of MLC3 to MLC2 in single type I fibers from the RUN group (P < 0.05), suggesting that the elevated Vzero of the RUN type I fibers was related to a greater expression of MLC3. In conclusion, the single fibers from the elite master runners displayed specific morphological and contractile properties that may enhance the performance of these athletes during prolonged muscular activity.
This article has been cited by other articles:
|
|
 |
|
  N. Luden, K. Minchev, E. Hayes, E. Louis, T. Trappe, and S. Trappe Human vastus lateralis and soleus muscles display divergent cellular contractile properties Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2008; 295(5): R1593 - R1598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Harber and S. Trappe Single muscle fiber contractile properties of young competitive distance runners J Appl Physiol, August 1, 2008; 105(2): 629 - 636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Slivka, U. Raue, C. Hollon, K. Minchev, and S. Trappe Single muscle fiber adaptations to resistance training in old (>80 yr) men: evidence for limited skeletal muscle plasticity Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2008; 295(1): R273 - R280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Trappe, A. Creer, K. Minchev, D. Slivka, E. Louis, N. Luden, and T. Trappe Human soleus single muscle fiber function with exercise or nutrition countermeasures during 60 days of bed rest Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R939 - R947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Malisoux, C. Jamart, K. Delplace, H. Nielens, M. Francaux, and D. Theisen Effect of long-term muscle paralysis on human single fiber mechanics J Appl Physiol, January 1, 2007; 102(1): 340 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Trappe, M. Harber, A. Creer, P. Gallagher, D. Slivka, K. Minchev, and D. Whitsett Single muscle fiber adaptations with marathon training J Appl Physiol, September 1, 2006; 101(3): 721 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Korhonen, A. Cristea, M. Alen, K. Hakkinen, S. Sipila, A. Mero, J. T. Viitasalo, L. Larsson, and H. Suominen Aging, muscle fiber type, and contractile function in sprint-trained athletes J Appl Physiol, September 1, 2006; 101(3): 906 - 917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Malisoux, M. Francaux, H. Nielens, and D. Theisen Stretch-shortening cycle exercises: an effective training paradigm to enhance power output of human single muscle fibers J Appl Physiol, March 1, 2006; 100(3): 771 - 779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. F. Coyle Improved muscular efficiency displayed as Tour de France champion matures J Appl Physiol, June 1, 2005; 98(6): 2191 - 2196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Canepari, R. Rossi, M. A. Pellegrino, R. W. Orrell, M. Cobbold, S. Harridge, and R. Bottinelli Effects of resistance training on myosin function studied by the in vitro motility assay in young and older men J Appl Physiol, June 1, 2005; 98(6): 2390 - 2395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Doucet, R. Debigare, D.R. Joanisse, C. Cote, P. LeBlanc, J. Gregoire, J. Deslauriers, R. Vaillancourt, and F. Maltais Adaptation of the diaphragm and the vastus lateralis in mild-to-moderate COPD Eur. Respir. J., December 1, 2004; 24(6): 971 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Harber, P. M. Gallagher, A. R. Creer, K. M. Minchev, and S. W. Trappe Single muscle fiber contractile properties during a competitive season in male runners Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2004; 287(5): R1124 - R1131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Widrick, G. F. Maddalozzo, D. Lewis, B. A. Valentine, D. P. Garner, J. E. Stelzer, T. C. Shoepe, and C. M. Snow Morphological and Functional Characteristics of Skeletal Muscle Fibers From Hormone-replaced and Nonreplaced Postmenopausal Women J. Gerontol. A Biol. Sci. Med. Sci., January 1, 2003; 58(1): B3 - 10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Widrick, J. E. Stelzer, T. C. Shoepe, and D. P. Garner Functional properties of human muscle fibers after short-term resistance exercise training Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2002; 283(2): R408 - R416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Trappe, M. Godard, P. Gallagher, C. Carroll, G. Rowden, and D. Porter Resistance training improves single muscle fiber contractile function in older women Am J Physiol Cell Physiol, August 1, 2001; 281(2): C398 - C406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Lambertz, C. Perot, R. Kaspranski, and F. Goubel Effects of long-term spaceflight on mechanical properties of muscles in humans J Appl Physiol, January 1, 2001; 90(1): 179 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Geiger, M. J. Cody, R. L. Macken, and G. C. Sieck Maximum specific force depends on myosin heavy chain content in rat diaphragm muscle fibers J Appl Physiol, August 1, 2000; 89(2): 695 - 703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Fitts, S. C. Bodine, J. G. Romatowski, and J. J. Widrick Velocity, force, power, and Ca2+ sensitivity of fast and slow monkey skeletal muscle fibers J Appl Physiol, May 1, 1998; 84(5): 1776 - 1787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. R. Harridge, R. Bottinelli, M. Canepari, M. Pellegrino, C. Reggiani, M. Esbjornsson, P. D. Balsom, and B. Saltin Sprint training, in vitro and in vivo muscle function, and myosin heavy chain expression J Appl Physiol, February 1, 1998; 84(2): 442 - 449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Widrick and R. H. Fitts Peak force and maximal shortening velocity of soleus fibers after non-weight-bearing and resistance exercise J Appl Physiol, January 1, 1997; 82(1): 189 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Widrick Effect of Pi on unloaded shortening velocity of slow and fast mammalian muscle fibers Am J Physiol Cell Physiol, April 1, 2002; 282(4): C647 - C653. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
