Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 271: C478-C485, 1996;
0363-6143/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Racette, K. J.
Right arrow Articles by Forsyth, G. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Racette, K. J.
Right arrow Articles by Forsyth, G. W.

AJP - Cell Physiology, Vol 271, Issue 2 C478-C485, Copyright © 1996 by American Physiological Society

ARTICLES

Monoclonal antibody against conductive chloride transport in pig ileal apical membrane vesicles

K. J. Racette, S. E. Gabriel, K. J. Gaspar and G. W. Forsyth
University of Saskatchewan, Saskatoon, Canada.

Conductive chloride transport in the small intestine is an important factor controlling fluid movement from the blood to the lumen of the gut. Several proteins with potential conductive chloride ion channel activity are expressed in the enterocyte cell population. However, it is not clear whether one or more than one protein species is normally responsible for mediating conductive chloride transport. We have raised monoclonal antibodies that inhibit conductive chloride transport in apical membrane vesicles prepared from porcine ileal enterocytes. These monoclonal antibodies have been used to identify a unique protein involved with this conductive chloride transport. Here, we report that anti-chloride conductance monoclonal antibodies did not detect any antigen in Western blots of enterocyte apical membrane protein. Dot blotting and immunoprecipitation experiments indicated that the antigen recognized by these monoclonal antibodies was not the cystic fibrosis transmembrane conductance regulator. The antigen was localized to both villus and crypt regions of ileum on immunohistochemistry. A 90-kDa protein species was immunoprecipitated from a primary enterocyte cell line by these monoclonal antibodies. This 90-kDa protein may be a chloride ion channel or may play some regulatory role in conductive chloride transport in enterocyte apical membrane vesicles.


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
M. E. Loewen and G. W. Forsyth
Structure and Function of CLCA Proteins
Physiol Rev, July 1, 2005; 85(3): 1061 - 1092.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. E. Loewen, S. E. Gabriel, and G. W. Forsyth
The calcium-dependent chloride conductance mediator pCLCA1
Am J Physiol Cell Physiol, August 1, 2002; 283(2): C412 - C421.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik
Molecular Structure and Physiological Function of Chloride Channels
Physiol Rev, April 1, 2002; 82(2): 503 - 568.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
K. J. GASPAR, K. J. RACETTE, J. R. GORDON, M. E. LOEWEN, and G. W. FORSYTH
Cloning a chloride conductance mediator from the apical membrane of porcine ileal enterocytes
Physiol Genomics, August 9, 2000; 3(2): 101 - 111.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online