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Am J Physiol Cell Physiol 271: C74-C84, 1996;
0363-6143/96 $5.00
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AJP - Cell Physiology, Vol 271, Issue 1 C74-C84, Copyright © 1996 by American Physiological Society

ARTICLES

Swelling and cAMP on hyperpolarization-activated Cl- conductance in rat Leydig cells

J. F. Noulin, E. Fayolle-Julien, J. F. Desaphy, J. P. Poindessault and M. Joffre
Laboratoire de Physiologie Animale, Unite de Recherche Associee Centre, National de la Recherche Scientifique 1869, Poitiers, France.

We have used the whole cell patch-clamp technique to characterize changes in membrane conductance induced by osmotic swelling in mature rat Leydig cells dialyzed with ATP (control cells) or adenosine 3',5'-cyclic monophosphate (cAMP) plus ATP (cAMP cells). A spontaneous current activation occurs in both groups in isosmotic conditions (300/295 mosM in/out). This development is entirely counteracted in control cells and partly inhibited in cAMP cells by exposing them to a hyperosmotic (350 mosM) bath solution, and these currents increase again in a hyposmotic (205 mosM) bath solution. These currents are sensitive to 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid, a Cl- channel blocker. Taken together, the results indicate that, in the control cells (ATP alone) as well as in the presence of intracellular cAMP, osmotic swelling activates the background hyperpolarization-activated Cl- conductance, osmotic swelling and cAMP appearing to act synergistically.


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