Am J Physiol Cell Physiol AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 270: C1370-C1378, 1996;
0363-6143/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Han, E. S.
Right arrow Articles by Reuss, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, E. S.
Right arrow Articles by Reuss, L.

AJP - Cell Physiology, Vol 270, Issue 5 C1370-C1378, Copyright © 1996 by American Physiological Society

ARTICLES

P-glycoprotein-associated chloride currents revealed by specific block by an anti-P-glycoprotein antibody

E. S. Han, C. G. Vanoye, G. A. Altenberg and L. Reuss
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston 77555-0641, USA.

The relationships between P-glycoprotein (PGP) expression and plasma membrane ion currents activated by cell swelling were studied in several cell lines by use of the whole cell configuration of the patch-clamp technique. Swelling-activated Cl- currents (ICls) had similar characteristics independently of whether PGP was expressed. Addition of the anti-PGP monoclonal antibody C219 or its Fab fragment to the pipette solution prevented ICls in cells expressing functional PGP (assessed by immunoblots, immunofluorescence, and transport of rhodamine 123) but not in cells lacking PGP expression. A peptide analogue of the C219 epitope abolished the effect of C219. Other anti-PGP antibodies and mouse immunoglobulin G were ineffective. C219 did not alter swelling-activated cation currents. Inasmuch as ICls is present in cells that do not express PGP and C219 has no effect on ICls in these cells, we conclude that PGP is not required for the ICls phenotype. However, when expressed in the plasma membrane, PGP is involved, directly or indirectly, in ICls but not in swelling-activated K+ currents.


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik
Molecular Structure and Physiological Function of Chloride Channels
Physiol Rev, April 1, 2002; 82(2): 503 - 568.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. H. Frank, M. D. Denton, S. I. Alexander, S. J. Khoury, M. H. Sayegh, and D. M. Briscoe
Specific MDR1 P-Glycoprotein Blockade Inhibits Human Alloimmune T Cell Activation In Vitro
J. Immunol., February 15, 2001; 166(4): 2451 - 2459.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. G. Vanoye, A. F. Castro, T. Pourcher, L. Reuss, and G. A. Altenberg
Phosphorylation of P-glycoprotein by PKA and PKC modulates swelling-activated Cl- currents
Am J Physiol Cell Physiol, February 1, 1999; 276(2): C370 - C378.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. S. Adorante, J. L. Edelman;, T. J. C. Jacob, and C. H. Mitchell
Letters to the Editor
Am J Physiol Cell Physiol, October 1, 1997; 273(4): C1435 - C1436.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online