Am J Physiol Cell Physiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 270: C1354-C1361, 1996;
0363-6143/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tahara, M.
Right arrow Articles by Miyake, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tahara, M.
Right arrow Articles by Miyake, A.

AJP - Cell Physiology, Vol 270, Issue 5 C1354-C1361, Copyright © 1996 by American Physiological Society

ARTICLES

Dynamics of cortical granule exocytosis at fertilization in living mouse eggs

M. Tahara, K. Tasaka, N. Masumoto, A. Mammoto, Y. Ikebuchi and A. Miyake
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.

Sperm-egg fusion induces an intracellular free calcium concentration ([Ca2+]i) increase and exocytosis of cortical granules (CGs). Recently we used an impermeable fluorescent membrane probe, 1-[4-(trimethylammonio)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH), to develop a method to evaluate the kinetics of exocytosis in single living cells. In this study we used digital imaging and confocal laser scanning microscopy to evaluate CG exocytosis in living mouse eggs with TMA-DPH. Time-related changes of CG exocytosis were estimated as the percent increase of TMA-DPH fluorescence. The increase of fluorescence in the egg started after sperm attachment, continued at an almost uniform rate, and ceased at 45-60 min. Whereas the [Ca2+]i increase at fertilization was transient or oscillatory, exocytosis was not always induced concomitantly with each [Ca2+]i peak. Next we used this method to determine some intracellular mediators of exocytosis in the egg. An intracellular calcium chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester, and a microfilament inhibitor, cytochalasin B, blocked sperm-induced exocytosis. A guanosine 5'-triphosphate-binding protein activator, AlF4-, induced exocytosis. These results suggest that [Ca2+]i, microfilament, and guanosine 5'-triphosphate-binding proteins may be involved in CG exocytosis. In conclusion, this method has significant advantages for studying exocytosis in living eggs.


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
M. Whitaker
Calcium at Fertilization and in Early Development
Physiol Rev, January 1, 2006; 86(1): 25 - 88.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
B. A. McAvey, G. B. Wortzman, C. J. Williams, and J. P. Evans
Involvement of Calcium Signaling and the Actin Cytoskeleton in the Membrane Block to Polyspermy in Mouse Eggs
Biol Reprod, October 1, 2002; 67(4): 1342 - 1352.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. L. Abbott, R. A. Fissore, and T. Ducibella
Identification of a Translocation Deficiency in Cortical Granule Secretion in Preovulatory Mouse Oocytes
Biol Reprod, December 1, 2001; 65(6): 1640 - 1647.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Ikebuchi, N. Masumoto, T. Matsuoka, T. Yokoi, M. Tahara, K. Tasaka, A. Miyake, and Y. Murata
SNAP-25 is essential for cortical granule exocytosis in mouse eggs
Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1496 - C1500.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online