| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 270, Issue 4 C1221-C1227, Copyright © 1996 by American Physiological Society
ARTICLES |
A. A. McDonough, Y. Zhang, V. Shin and J. S. Frank
Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033, USA.
The cardiac Na+ pump (Na+ -K+ -ATPase) provides the driving force for the Na+/Ca2+ exchanger, a determinant of intracellular Ca2+ stores. Three Na+ pump alpha-catalytic subunit isoforms are expressed in human heart, alpha1 and alpha2 are expressed in rat heart, and only alpha1 is expressed in guinea pig heart. The objective of this study was to determine whether there are isoform-specific patterns of expression in the transverse tubules (T tubules) vs. the peripheral sarcolemma. In adult rat cardiomyocytes, anti-alpha1-specific antibodies labeled the T tubules more intensely than the peripheral sarcolemma, in which labeling was patchy, the same pattern reported for distribution of the Na+/Ca2+ exchanger (J. S. Frank, G. Mottino, D. Reid, R. S. Molday, and K. D. Philipson, J. Cell Biol. 117: 337-345, 1992), whereas anti-alpha2- and anti-beta1-antibodies uniformly labeled T tubules and peripheral sarcolemma. In guinea pig cardiomyocytes, an anti-alpha-antibody against an extracellular epitope evenly labeled the peripheral sarcolemma and T tubules, and immunogold labeling demonstrated coincidence of alpha-subunits and intramembranous particles in sarcolemma. In summary, Na+ pumps are located in both peripheral sarcolemma and T tubules of cardiomyocytes expressing either multiple or single Na+ pump isoforms.
This article has been cited by other articles:
|
|
 |
|
  S. Despa and D. M. Bers Functional analysis of Na+/K+-ATPase isoform distribution in rat ventricular myocytes Am J Physiol Cell Physiol, July 1, 2007; 293(1): C321 - C327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu and A. Askari beta-Subunit of cardiac Na+-K+-ATPase dictates the concentration of the functional enzyme in caveolae Am J Physiol Cell Physiol, October 1, 2006; 291(4): C569 - C578. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Q. Zhang, J. R. Moorman, B. A. Ahlers, L. L. Carl, D. E. Lake, J. Song, J. P. Mounsey, A. L. Tucker, Y.-m. Chan, L. I. Rothblum, et al. Phospholemman overexpression inhibits Na+-K+-ATPase in adult rat cardiac myocytes: relevance to decreased Na+ pump activity in postinfarction myocytes J Appl Physiol, January 1, 2006; 100(1): 212 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Wasserstrom and G. L. Aistrup Digitalis: new actions for an old drug Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H1781 - H1793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-G. Jia, C. Donnet, R. C. Bogaev, R. J. Blatt, C. E. McKinney, K. H. Day, S. S. Berr, L. R. Jones, J. R. Moorman, K. J. Sweadner, et al. Hypertrophy, increased ejection fraction, and reduced Na-K-ATPase activity in phospholemman-deficient mice Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1982 - H1988. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. CLAUSEN Na+-K+ Pump Regulation and Skeletal Muscle Contractility Physiol Rev, October 1, 2003; 83(4): 1269 - 1324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. G. Glitsch Electrophysiology of the Sodium-Potassium-ATPase in Cardiac Cells Physiol Rev, October 1, 2001; 81(4): 1791 - 1826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Muller-Ehmsen, P. Juvvadi, C. B. Thompson, L. Tumyan, M. Croyle, J. B. Lingrel, R. H. G. Schwinger, A. A. McDonough, and R. A. Farley Ouabain and substrate affinities of human Na+-K+-ATPase alpha 1beta 1, alpha 2beta 1, and alpha 3beta 1 when expressed separately in yeast cells Am J Physiol Cell Physiol, October 1, 2001; 281(4): C1355 - C1364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kometiani, A. Askari, J. Liu, Z. Xie, and F. K. Askari Downregulation of cardiac myocyte Na+-K+-ATPase by adenovirus-mediated expression of an {alpha}-subunit fragment Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H1415 - H1421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Sejersted and G. Sjogaard Dynamics and Consequences of Potassium Shifts in Skeletal Muscle and Heart During Exercise Physiol Rev, October 1, 2000; 80(4): 1411 - 1481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Trouve, F. Carre, I. Belikova, C. Leclercq, T. Dakhli, L. Soufir, I. Coquard, J. Ramirez-Gil, and D. Charlemagne Na+-K+-ATPase alpha 2-isoform expression in guinea pig hearts during transition from compensation to decompensation Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1972 - H1981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Laflamme and P. L. Becker Gs and adenylyl cyclase in transverse tubules of heart: implications for cAMP-dependent signaling Am J Physiol Heart Circ Physiol, November 1, 1999; 277(5): H1841 - H1848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Petrecca, R. Atanasiu, S. Grinstein, J. Orlowski, and A. Shrier Subcellular localization of the Na+/H+ exchanger NHE1 in rat myocardium Am J Physiol Heart Circ Physiol, February 1, 1999; 276(2): H709 - H717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Su, A. Zou, A. Nonaka, I. Zubair, M. C. Sanguinetti, and W. H. Barry Influence of prior Na+ pump activity on pump and Na+/Ca2+ exchange currents in mouse ventricular myocytes Am J Physiol Heart Circ Physiol, November 1, 1998; 275(5): H1808 - H1817. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Juhaszova and M. P. Blaustein Na+ pump low and high ouabain affinity alpha subunit isoforms are differently distributed in cells PNAS, March 4, 1997; 94(5): 1800 - 1805. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
