Am J Physiol Cell Physiol Journal of Applied Physiology
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Am J Physiol Cell Physiol 270: C740-C747, 1996;
0363-6143/96 $5.00
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AJP - Cell Physiology, Vol 270, Issue 3 C740-C747, Copyright © 1996 by American Physiological Society

ARTICLES

Protein kinase C modulates natriuretic peptide receptors in astroglial cultures from rat brain

W. R. Paulding and C. Sumners
Department of Physiology, College of Medicine, University of Florida, Gainsville 32610, USA.

We determined previously that astroglia cultured from newborn rat brain contain both guanylyl cyclase-coupled and atrial natriuretic peptide (ANP)-C natriuretic peptide receptors. Here, we investigated the effects of the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) on these receptor subtypes in cultured astroglia to understand the intracellular processes involved in the modulation of natriuretic peptide receptors in these cells. PMA (10 nM to 1 microM; 15 min to 24 h) treatment elicited a time- and concentration-dependent decrease in the numbers of 125I-labeled ANP specific binding sites, which was inhibited by the PKC antagonist staurosporine (500 nM). Furthermore, PMA (100 nM, 2 or 24 h) treatment elicited a significant decrease in the specific binding of 125I-des-Cys-Cys-ANP, an ANP-C receptor selective ligand. PMA (10 nM to 1 microM; 30 min) treatment also significantly decreased ANP (100 nM)-stimulated guanosine 3', 5'-cyclic monophosphate levels in cultured astroglia, an effect unmodified by phosphodiesterase inhibition. These data indicate that PKC modulates both guanylyl cyclase-coupled and ANP-C natriuretic peptide receptors in cultured astroglia.


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S. E. Abbey and L. R. Potter
Vasopressin-dependent Inhibition of the C-type Natriuretic Peptide Receptor, NPR-B/GC-B, Requires Elevated Intracellular Calcium Concentrations
J. Biol. Chem., November 1, 2002; 277(45): 42423 - 42430.
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