Am J Physiol Cell Physiol AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 270: C259-C264, 1996;
0363-6143/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xu, X. M.
Right arrow Articles by Wu, K. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xu, X. M.
Right arrow Articles by Wu, K. K.

AJP - Cell Physiology, Vol 270, Issue 1 C259-C264, Copyright © 1996 by American Physiological Society

ARTICLES

Transcriptional regulation of endothelial constitutive PGHS-1 expression by phorbol ester

X. M. Xu, J. L. Tang, A. Hajibeigi, D. S. Loose-Mitchell and K. K. Wu
Department of Internal Medicine, University of Texas Houston Health Science Center, Houston 77030, USA.

Human endothelial cells contain two isoforms of prostaglandin H synthase (PGHS). PGHS-1 is constitutively expressed, whereas PGHS-2 is inducible. To determine whether expression of PGHS-1 is regulated, we treated cultured human umbilical vein endothelial cells (HUVEC) with phorbol 12-myristate 13-acetate (PMA) or its inactive analogue and measured PGHS-1 mRNA levels by Northern analysis and competitive polymerase chain reaction. PMA increased PGHS-1 mRNA levels determined by both techniques in a time- and concentration-dependent manner. The mRNA level was increased about twofold over the basal level after 4-6 h of PMA (10-50 nM) treatment. The level of PGHS-1 protein was similarly increased by PMA. Stimulation of PGHS-1 mRNA levels was abrogated by cycloheximide, actinomycin D, staurosporine, or calphostin C. The 5'-promoter activity of human PGHS-1 gene was increased twofold over the basal level by PMA in NS-20 cells. These results indicate that the constitutive PGHS-1 in HUVEC is transcriptionally stimulated by PMA in a protein kinase C-dependent manner.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Taniura, H. Kamitani, T. Watanabe, and T. E. Eling
Transcriptional Regulation of Cyclooxygenase-1 by Histone Deacetylase Inhibitors in Normal Human Astrocyte Cells
J. Biol. Chem., May 3, 2002; 277(19): 16823 - 16830.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
H.-D. Orzechowski, A. Gunther, S. Menzel, A. Zimmermann, H. Funke-Kaiser, R. Real, T. Subkowski, F. S. Zollmann, and M. Paul
Transcriptional Mechanism of Protein Kinase C-Induced Isoform-Specific Expression of the Gene for Endothelin-Converting Enzyme-1in Human Endothelial Cells
Mol. Pharmacol., December 1, 2001; 60(6): 1332 - 1342.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. G. Gianoukakis, H. J. Cao, T. A. Jennings, and T. J. Smith
Prostaglandin endoperoxide H synthase expression in human thyroid epithelial cells
Am J Physiol Cell Physiol, March 1, 2001; 280(3): C701 - C708.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. J. Cao and T. J. Smith
Leukoregulin upregulation of prostaglandin endoperoxide H synthase-2 expression in human orbital fibroblasts
Am J Physiol Cell Physiol, December 1, 1999; 277(6): C1075 - C1085.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X.-M. Xu, L. Sansores-Garcia, X.-M. Chen, N. Matijevic-Aleksic, M. Du, and K. K. Wu
Suppression of inducible cyclooxygenase 2 gene transcription by aspirin and sodium salicylate
PNAS, April 27, 1999; 96(9): 5292 - 5297.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. W. Shaul, M. C. Pace, Z. Chen, and T. S. Brannon
Developmental Changes in Prostacyclin Synthesis Are Conserved in Cultured Pulmonary Endothelium and Vascular Smooth Muscle
Am. J. Respir. Cell Mol. Biol., January 1, 1999; 20(1): 113 - 121.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
K. Okahara, B. Sun, and J.-i. Kambayashi
Upregulation of Prostacyclin Synthesis–Related Gene Expression by Shear Stress in Vascular Endothelial Cells
Arterioscler Thromb Vasc Biol, December 1, 1998; 18(12): 1922 - 1926.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. S. Brannon, A. N. MacRitchie, M. A. Jaramillo, T. S. Sherman, I. S. Yuhanna, L. R. Margraf, and P. W. Shaul
Ontogeny of cyclooxygenase-1 and cyclooxygenase-2 gene expression in ovine lung
Am J Physiol Lung Cell Mol Physiol, January 1, 1998; 274(1): L66 - L71.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-M. Xu, J.-L. Tang, X. Chen, L.-H. Wang, and K. K.-y. Wu
Involvement of Two Sp1 Elements in Basal Endothelial Prostaglandin H Synthase-1 Promoter Activity
J. Biol. Chem., March 14, 1997; 272(11): 6943 - 6950.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-Y. Liou, W.-G. Deng, D. W. Gilroy, S.-K. Shyue, and K. K. Wu
Colocalization and Interaction of Cyclooxygenase-2 with Caveolin-1 in Human Fibroblasts
J. Biol. Chem., September 7, 2001; 276(37): 34975 - 34982.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online