| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 270, Issue 1 C243-C252, Copyright © 1996 by American Physiological Society
ARTICLES |
D. Mehta, M. F. Wu and S. J. Gunst
Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis 46202, USA.
The active isometric force developed by a muscle decreases at muscle lengths below an optimal length (Lo). However, when the length of an actively contracting muscle is abruptly decreased, a lower level of isometric force is reached during force redevelopment than when the contraction is initiated at the shorter length. This has been attributed to a deactivation of contractile proteins caused by shortening. In this study, intracellular Ca2+ and myosin light chain (MLC) phosphorylation were measured to assess the mechanisms for the modulation of isometric force caused by changing smooth muscle length before or during isometric contraction. The decline in isometric force between Lo and 0.5Lo was associated with decreases in MLC phosphorylation and intracellular Ca2+ during contractions elicited by acetylcholine or 60 mM KCl. Quick release of the muscle during contraction depressed force redevelopment at the shorter length but not MLC phosphorylation. We conclude that decreases in Ca(2+)-calmodulin-dependent MLC phosphorylation contribute significantly to the decline in isometric force at lengths below Lo, but the depression of contractility associated with the quick release of actively contracted smooth muscle is not caused by a shortening-induced deactivation of contractile proteins.
This article has been cited by other articles:
|
|
 |
|
  Z. Xue, L. Zhang, Y. Liu, S. J. Gunst, and R. S. Tepper Chronic inflation of ferret lungs with CPAP reduces airway smooth muscle contractility in vivo and in vitro J Appl Physiol, March 1, 2008; 104(3): 610 - 615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Ford and S. H. Gilbert The significance of variable passive compliance in smooth muscle J Appl Physiol, May 1, 2007; 102(5): 1735 - 1736. [Full Text] [PDF]
|
|
|
|
|
|
A. V. Smolensky and L. E. Ford The extensive length-force relationship of porcine airway smooth muscle J Appl Physiol, May 1, 2007; 102(5): 1906 - 1911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. P. Silveira, J. P. Butler, and J. J. Fredberg Length adaptation of airway smooth muscle: a stochastic model of cytoskeletal dynamics J Appl Physiol, December 1, 2005; 99(6): 2087 - 2098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Lambert and T. A. Wilson Smooth muscle dynamics and maximal expiratory flow in asthma J Appl Physiol, November 1, 2005; 99(5): 1885 - 1890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Fernandes, R. W. Mitchell, O. Lakser, M. Dowell, A. G. Stewart, and J. Solway Invited Review: Do inflammatory mediators influence the contribution of airway smooth muscle contraction to airway hyperresponsiveness in asthma? J Appl Physiol, August 1, 2003; 95(2): 844 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. G. Smith, L. Deng, J. J. Fredberg, and G. N. Maksym Mechanical strain increases cell stiffness through cytoskeletal filament reorganization Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L456 - L463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Weist, T. Williams, J. Kisling, C. Clem, and R. S. Tepper Volume history and effect on airway reactivity in infants and adults J Appl Physiol, September 1, 2002; 93(3): 1069 - 1074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Gunst, X. Shen, R. Ramchandani, and R. S. Tepper Bronchoprotective and bronchodilatory effects of deep inspiration in rabbits subjected to bronchial challenge J Appl Physiol, December 1, 2001; 91(6): 2511 - 2516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Tang and S. J. Gunst Signal Transduction in Smooth Muscle: Selected Contribution: Roles of focal adhesion kinase and paxillin in the mechanosensitive regulation of myosin phosphorylation in smooth muscle J Appl Physiol, September 1, 2001; 91(3): 1452 - 1459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yoshimura, K. A. Jones, W. J. Perkins, T. Kai, and D. O. Warner Calcium sensitization produced by G protein activation in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L631 - L638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. Gerthoffer and S. J. Gunst Signal Transduction in Smooth Muscle: Invited Review: Focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle J Appl Physiol, August 1, 2001; 91(2): 963 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Tang and S. J. Gunst Depletion of focal adhesion kinase by antisense depresses contractile activation of smooth muscle Am J Physiol Cell Physiol, April 1, 2001; 280(4): C874 - C883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Halayko and J. Solway Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Molecular mechanisms of phenotypic plasticity in smooth muscle cells J Appl Physiol, January 1, 2001; 90(1): 358 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Seow Response of arterial smooth muscle to length perturbation J Appl Physiol, November 1, 2000; 89(5): 2065 - 2072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. An and C.-M. Hai Mechanical signals and mechanosensitive modulation of intracellular [Ca2+] in smooth muscle Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1375 - C1384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kai, H. Yoshimura, K. A. Jones, and D. O. Warner Relationship between force and regulatory myosin light chain phosphorylation in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L52 - L58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-L. Chan, J. Silberstein, and C.-M. Hai Mechanical strain memory in airway smooth muscle Am J Physiol Cell Physiol, May 1, 2000; 278(5): C895 - C904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. An and C.-M. Hai Mechanical strain modulates maximal phosphatidylinositol turnover in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, November 1, 1999; 277(5): L968 - L974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Davis and M. A. Hill Signaling Mechanisms Underlying the Vascular Myogenic Response Physiol Rev, April 1, 1999; 79(2): 387 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-X. Blanc, S. Salmeron, C. Coirault, M. Bard, E. Fadel, E. Dulmet, P. Dartevelle, and Y. Lecarpentier Effects of load and tone on the mechanics of isolated human bronchial smooth muscle J Appl Physiol, February 1, 1999; 86(2): 488 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Fredberg and S. A. Shore Invited Editorial on "Influence of intercellular tissue connections on airway muscle mechanics" J Appl Physiol, January 1, 1999; 86(1): 3 - 4. [Full Text] [PDF]
|
|
|
|
|
|
R. A. Meiss Influence of intercellular tissue connections on airway muscle mechanics J Appl Physiol, January 1, 1999; 86(1): 5 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Tang, D. Mehta, and S. J. Gunst Mechanosensitive tyrosine phosphorylation of paxillin and focal adhesion kinase in tracheal smooth muscle Am J Physiol Cell Physiol, January 1, 1999; 276(1): C250 - C258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bard, S. Salmeron, C. Coirault, F.-X. Blanc, and Y. Lecarpentier Effects of initial length on intrinsic tone in guinea pig tracheal smooth muscle Am J Physiol Lung Cell Mol Physiol, December 1, 1998; 275(6): L1026 - L1030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Youn, S. A. Kim, and C.-M. Hai Length-dependent modulation of smooth muscle activation: effects of agonist, cytochalasin, and temperature Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1601 - C1607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shen, S. J. Gunst, and R. S. Tepper Effect of tidal volume and frequency on airway responsiveness in mechanically ventilated rabbits J Appl Physiol, October 1, 1997; 83(4): 1202 - 1208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shen, M. F. Wu, R. S. Tepper, and S. J. Gunst Mechanisms for the mechanical response of airway smooth muscle to length oscillation J Appl Physiol, September 1, 1997; 83(3): 731 - 738. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
