Am J Physiol Cell Physiol Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 269: C1408-C1416, 1995;
0363-6143/95 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kolyada, A. Y.
Right arrow Articles by Madias, N. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kolyada, A. Y.
Right arrow Articles by Madias, N. E.

AJP - Cell Physiology, Vol 269, Issue 6 C1408-C1416, Copyright © 1995 by American Physiological Society

ARTICLES

Role of C/EBP proteins in hepatic and vascular smooth muscle transcription of human NHE1 gene

A. Y. Kolyada, C. A. Johns and N. E. Madias
Department of Medicine, Tufts University School of Medicine, Boston, Massachusetts, USA.

We have recently shown that regulatory element D (nucleotides -239 to -215) of the 0.25-kb promoter of the human growth factor-activatable Na+/H+ exchanger (NHE1) is important for gene transcription in cells of hepatic origin (Hep G2) and vascular smooth muscle origin (VSM A7r5). This element contains a sequence (nucleotides -230 to -222) with complete homology to the C/EBP binding site. We now demonstrate that nucleotide substitution mutations disrupting this C/EBP site suppressed transcription in Hep G2 cells, VSM A7r5 cells, and Sprague-Dawley VSM cells in primary culture. These mutations abolished the binding of rat liver nuclear activities as well as transcription factors C/EBP alpha, C/EBP beta, and C/EBP delta expressed in COS-1 cell lysates to element D. Anti-C/EBP antibodies supershifted DNA-protein complexes formed between hepatic nuclear activities or C/EBP proteins expressed in COS-1 cell lysates and regulatory element D. Finally, cotransfection experiments of NHE1 0.25-kb promoter-chloramphenicol acetyltransferase (CAT) construct and C/EBP expression vectors showed that C/EBP alpha and C/EBP delta are transactivators of the NHE1 proximal promoter in Hep G2 and VSM A7r5 cells. These results indicate that members of the C/EBP family of transcription factors are involved in the regulation of hepatic and vascular smooth muscle transcription of the human NHE1 gene.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
X. Li, A. J. Misik, C. V. Rieder, R. J. Solaro, A. Lowen, and L. Fliegel
Thyroid Hormone Receptor alpha 1 Regulates Expression of the Na+/H+ Exchanger (NHE1)
J. Biol. Chem., August 2, 2002; 277(32): 28656 - 28662.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Fukuoka, Y. Kitami, T. Okura, and K. Hiwada
Transcriptional Regulation of the Platelet-derived Growth Factor alpha Receptor Gene via CCAAT/Enhancer-binding Protein-delta in Vascular Smooth Muscle Cells
J. Biol. Chem., September 3, 1999; 274(36): 25576 - 25582.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
Y. Kitami, T. Fukuoka, K. Hiwada, and T. Inagami
A High Level of CCAAT-Enhancer Binding Protein-{delta} Expression Is a Major Determinant for Markedly Elevated Differential Gene Expression of the Platelet-Derived Growth Factor-{alpha} Receptor in Vascular Smooth Muscle Cells of Genetically Hypertensive Rats
Circ. Res., January 22, 1999; 84(1): 64 - 73.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online