Am J Physiol Cell Physiol AJP: Cell Physiology
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Am J Physiol Cell Physiol 269: C923-C928, 1995;
0363-6143/95 $5.00
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AJP - Cell Physiology, Vol 269, Issue 4 C923-C928, Copyright © 1995 by American Physiological Society

ARTICLES

Ion microscopy evidence that La3+ releases Ca2+ from Golgi complex in LLC-PK1 cells

X. Zha and G. H. Morrison
Baker Laboratory, Department of Chemistry, Cornell University, Ithaca, New York 14853, USA.

The effect of La3+ on LLC-PK1 cells was investigated by ion microscopy, a mass spectrometry-based technique with a spatial resolution of approximately 0.5 micron. Cells were incubated with LaCl3 for 10 min. (1 mM) or 30 min (0.1 mM), and intracellular calcium distributions were measured with a Cameca IMS-3f ion microscope in cryogenically prepared cells. Compared with control cells, La3+ reduced total calcium in the Golgi complex by > 100 microM in both treatments, whereas other cellular regions, such as the nucleus and cytoplasm, remained largely unchanged. These two treatments were repeated on cells that were preincubated with 1 mM ouabain. The presence of ouabain in the medium increased the loss of calcium from the Golgi by about fourfold compared with the treatments without ouabain. The La3+ effect, therefore, was amplified by ouabain-induced Na+ loading, indicating a possible involvement of a Na+/La3+ exchanger. La3+ was detected within cells and its influx was facilitated by Na+ loading. These results suggest that La3+ may affect cellular calcium homeostasis by actions other than as a simple Ca2+ antagonist.


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