AJP - Cell Physiology, Vol 269, Issue 3 C797-C801, Copyright © 1995 by American Physiological Society

ARTICLES

Water channel-carrying vesicles in the rat IMCD contain cellubrevin

N. Franki, F. Macaluso, W. Schubert, L. Gunther and R. M. Hays
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Antidiuretic hormone (arginine vasopressin) induces a cyclic process of docking, fusion, and endocytosis of water channel-containing vesicles in the collecting duct. There is now evidence that docking and endocytosis are mediated by an array of proteins associated with vesicles and target membranes. In recent studies, we have shown that cellubrevin, a member of the vesicle-associated membrane protein family, as well as other docking proteins, are expressed in the rat inner medullary collecting duct. We now show by immunogold electron microscopy that cellubrevin is present on vesicles containing water channels, that it is associated with both coated and uncoated vesicles, and that it is present on the apical membrane. Cellubrevin, therefore, is in a position to mediate one or more steps in arginine vasopressin-induced water channel cycling.

This article has been cited by other articles:

				P. Uawithya, T. Pisitkun, B. E. Ruttenberg, and M. A. Knepper Transcriptional profiling of native inner medullary collecting duct cells from rat kidney Physiol Genomics, January 17, 2008; 32(2): 229 - 253. [Abstract] [Full Text] [PDF]

				C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel Renal Vacuolar H+-ATPase Physiol Rev, October 1, 2004; 84(4): 1263 - 1314. [Abstract] [Full Text] [PDF]

				S. Breton, N. N. Nsumu, T. Galli, I. Sabolic, P. J. S. Smith, and D. Brown Tetanus toxin-mediated cleavage of cellubrevin inhibits proton secretion in the male reproductive tract Am J Physiol Renal Physiol, May 1, 2000; 278(5): F717 - F725. [Abstract] [Full Text] [PDF]

				C. YEAMAN, K. K. GRINDSTAFF, and W. J. NELSON New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity Physiol Rev, January 1, 1999; 79(1): 73 - 98. [Abstract] [Full Text] [PDF]

				T. Inoue, S. Nielsen, B. Mandon, J. Terris, B. K. Kishore, and M. A. Knepper SNAP-23 in rat kidney: colocalization with aquaporin-2 in collecting duct vesicles Am J Physiol Renal Physiol, November 1, 1998; 275(5): F752 - F760. [Abstract] [Full Text] [PDF]

				S. D'Souza, A. Garcia-Cabado, F. Yu, K. Teter, G. Lukacs, K. Skorecki, H.-P. Moore, J. Orlowski, and S. Grinstein The Epithelial Sodium-Hydrogen Antiporter Na+/H+ Exchanger 3 Accumulates and Is Functional in Recycling Endosomes J. Biol. Chem., January 23, 1998; 273(4): 2035 - 2043. [Abstract] [Full Text] [PDF]

				P. Lledo, X. Zhang, T. C. Südhof, R. C. Malenka, and R. A. Nicoll Postsynaptic Membrane Fusion and Long-Term Potentiation Science, January 16, 1998; 279(5349): 399 - 403. [Abstract] [Full Text]

				E. A. Alexander, T. Shih, and J. H. Schwartz H+ secretion is inhibited by clostridial toxins in an inner medullary collecting duct cell line Am J Physiol Renal Physiol, December 1, 1997; 273(6): F1054 - F1057. [Abstract] [Full Text] [PDF]