| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 269, Issue 1 C134-C140, Copyright © 1995 by American Physiological Society
ARTICLES |
Z. Karim, N. Defontaine, M. Paillard and J. Poggioli
Institut National de la Sante et de la Recherche Medicale U356, Universite Paris VI, France.
The present study examined the effect of phorbol esters, Ca2+, and angiotensin II (ANG II) on protein kinase C (PKC) isoforms in the rat proximal tubule. The immunoblot analysis of PKC isoforms of particulate and cytosolic fractions of proximal tubules revealed immunoreactive proteins when antibodies against PKC-alpha, -delta, -epsilon, and -zeta, but not -beta and -gamma were used. Phorbol dibutyrate (PDBU) induced the translocation of PKC-alpha, -delta, and -epsilon, whereas an inactive phorbol ester had no effect. PDBU and ionomycin increased particulate PKC specific activity from 0.67 +/- 0.09 to 1.56 +/- 0.18 and 0.96 +/- 0.04 pmol.microgram protein-1.2 min-1, respectively. ANG II (10(-7) M) induced a time-dependent increase in particulate PKC-alpha immunoreactivity observed after 2 min and maintained for 12 min. Particulate PKC-epsilon immunoreactivity increased after 4 min. Meanwhile, PKC-delta and -zeta were not modified by ANG II. Accordingly, ANG II elicited a rise in the specific activity of the particulate PKC, which increased to 0.89 +/- 0.09 pmol.micrograms protein-1.2 min-1 after 2 min. This was inhibited by a preincubation in the presence of 10(-5) M losartan, specific inhibitor of angiotensin subtype 1 receptors. These data indicate that PKC-alpha and -epsilon are potential candidates to regulate the activity of Na+/H+ and Na(+)-HCO3- transporters because they are translocated with a time course fitting with that of the reported effect of ANG II on those transporters.
This article has been cited by other articles:
|
|
 |
|
  R. A. Fenton and M. A. Knepper Mouse Models and the Urinary Concentrating Mechanism in the New Millennium Physiol Rev, October 1, 2007; 87(4): 1083 - 1112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kirchheimer, C. F. Mendez, A. Acquier, and S. Nowicki Role of 20-HETE in D1/D2 dopamine receptor synergism resulting in the inhibition of Na+-K+-ATPase activity in the proximal tubule Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1435 - F1442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-J. Lee, I.-K. Song, K.-J. Jang, J. Nielsen, J. Frokiaer, S. Nielsen, and T.-H. Kwon Increased AQP2 targeting in primary cultured IMCD cells in response to angiotensin II through AT1 receptor Am J Physiol Renal Physiol, January 1, 2007; 292(1): F340 - F350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Nielsen, T.-H. Kwon, J. Frokiaer, M. A. Knepper, and S. Nielsen Maintained ENaC trafficking in aldosterone-infused rats during mineralocorticoid and glucocorticoid receptor blockade Am J Physiol Renal Physiol, January 1, 2007; 292(1): F382 - F394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Perry, J. Blaine, H. Le, and I. I. Grichtchenko PMA- and ANG II-induced PKC regulation of the renal Na+-HCO3- cotransporter (hkNBCe1) Am J Physiol Renal Physiol, February 1, 2006; 290(2): F417 - F427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Redling, I. L. Pfaff, M. Leitges, and V. Vallon Immunolocalization of protein kinase C isoenzymes {alpha}, {beta}I, {beta}II, {delta}, and {varepsilon} in mouse kidney Am J Physiol Renal Physiol, August 1, 2004; 287(2): F289 - F298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yao, D.-Y. Huang, I. L. Pfaff, X. Nie, M. Leitges, and V. Vallon Evidence for a role of protein kinase C-{alpha} in urine concentration Am J Physiol Renal Physiol, August 1, 2004; 287(2): F299 - F304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Velic, J. R. Hirsch, J. Bartel, R. Thomas, R. Schroter, H. Stegemann, B. Edemir, C. August, E. Schlatter, and G. Gabriels Renal Transplantation Modulates Expression and Function of Receptors and Transporters of Rat Proximal Tubules J. Am. Soc. Nephrol., April 1, 2004; 15(4): 967 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Asghar, T. Hussain, and M. F. Lokhandwala Overexpression of PKC-{beta}I and -{delta} contributes to higher PKC activity in the proximal tubules of old Fischer 344 rats Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1100 - F1107. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. I. Gonzalez, M. G. Kazanietz, and M. B. Robinson Regulation of the Neuronal Glutamate Transporter Excitatory Amino Acid Carrier-1 (EAAC1) by Different Protein Kinase C Subtypes Mol. Pharmacol., October 1, 2002; 62(4): 901 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Awayda, J. D. Platzer, R. L. Reger, and A. Bengrine Role of PKCalpha in feedback regulation of Na+ transport in an electrically tight epithelium Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1122 - C1132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Boesch and J. L. Garvin Age-dependent activation of PKC isoforms by angiotensin II in the proximal nephron Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2001; 281(3): R861 - R867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Buhagiar, P. S. Hansen, N. L. Bewick, and H. H. Rasmussen Protein kinase C{varepsilon} contributes to regulation of the sarcolemmal Na+-K+ pump Am J Physiol Cell Physiol, September 1, 2001; 281(3): C1059 - C1063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. D. Millar and L. Robson Na+-alanine uptake activates a Cl{-} conductance in frog renal proximal tubule cells via nonconventional PKC Am J Physiol Renal Physiol, May 1, 2001; 280(5): F758 - F767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kato, J. D. Klein, C. Zhang, and J. M. Sands Angiotensin II increases vasopressin-stimulated facilitated urea permeability in rat terminal IMCDs Am J Physiol Renal Physiol, November 1, 2000; 279(5): F835 - F840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. L. PFAFF, H.-J. WAGNER, and V. VALLON Immunolocalization of Protein Kinase C Isoenzymes {alpha}, {beta}I and {beta}II in Rat Kidney J. Am. Soc. Nephrol., September 1, 1999; 10(9): 1861 - 1873. [Abstract] [Full Text]
|
|
|
|
 |
|
 Z. Jobbagy, Z. Olah, G. Petrovics, M. V. Eiden, B. D. Leverett, N. M. Dean, and W. B. Anderson Up-regulation of the Pit-2 Phosphate Transporter/Retrovirus Receptor by Protein Kinase C epsilon J. Biol. Chem., March 12, 1999; 274(11): 7067 - 7071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. P. Yao, X. X. Li, P.-Y. Yu, J. Xu, L. D. Asico, and P. A. Jose Dopamine D1 Receptor and Protein Kinase C Isoforms in Spontaneously Hypertensive Rats Hypertension, December 1, 1998; 32(6): 1049 - 1053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Hohage, A. Stachon, C. Feidt, J. R. Hirsch, and E. Schlatter Regulation of Organic Cation Transport in IHKE-1 and LLC-PK1 Cells. Fluorometric Studies with 4-(4-Dimethylaminostyryl)N-methylpyridinium J. Pharmacol. Exp. Ther., July 1, 1998; 286(1): 305 - 310. [Abstract] [Full Text]
|
|
|
|
|
|
H. Amlal, C. LeGoff, C. Vernimmen, M. Soleimani, M. Paillard, and M. Bichara ANG II controls Na+-K+(NH+4)-2Cl- cotransport via 20-HETE and PKC in medullary thick ascending limb Am J Physiol Cell Physiol, April 1, 1998; 274(4): C1047 - C1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-L. Chou, S. I. Rapko, and M. A. Knepper Phosphoinositide signaling in rat inner medullary collecting duct Am J Physiol Renal Physiol, March 1, 1998; 274(3): F564 - F572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Franchi-Gazzola, R. Visigalli, O. Bussolati, and G. C. Gazzola Involvement of Protein Kinase Cepsilon in the Stimulation of Anionic Amino Acid Transport in Cultured Human Fibroblasts J. Biol. Chem., October 18, 1996; 271(42): 26124 - 26130. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
