Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 268: C1512-C1519, 1995;
0363-6143/95 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, J. G.
Right arrow Articles by Kempson, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, J. G.
Right arrow Articles by Kempson, S. A.

AJP - Cell Physiology, Vol 268, Issue 6 C1512-C1519, Copyright © 1995 by American Physiological Society

ARTICLES

Microtubule disruption stimulates system A transport in cultured vascular smooth muscle cells

J. G. Chen, A. B. Strawbridge and S. A. Kempson
Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis 46202-5120, USA.

This study has focused on the possible influence of microtubules for the regulation of Na(+)-dependent system A neutral amino acid transport in A10 cells, a cultured cell line derived from rat aortic vascular smooth muscle. When microtubules were disrupted by incubating cells for 5 h in serum-free medium containing colchicine, nocodazole, or vinblastine, there was a twofold increase in system A transport (Vmax change). The dose for the disruption of microtubules by colchicine was similar to the dose required for the stimulation of system A. The time course showed that system A stimulation did not occur until widespread disruption of microtubules was established. The stimulation was specific for system A; there were no changes in glucose transport and Na(+)-dependent transport of phosphate and glutamate. Serum refeeding of quiescent cells from 2 days of serum starvation led to stimulation of system A, glucose, and phosphate transport. However, only system A was activated when colchicine was added to the serum-free medium. Addition of colchicine during serum refeeding had no additive effect for the stimulation of system A. The stimulation by both colchicine and serum was blocked by cycloheximide and actinomycin D. These findings suggest that microtubule disruption may activate system A gene expression.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
S. A. Kempson, V. Parikh, L. Xi, S. Chu, and M. H. Montrose
Subcellular redistribution of the renal betaine transporter during hypertonic stress
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1091 - C1100.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. E. Mann, D. L. Yudilevich, and L. Sobrevia
Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells
Physiol Rev, January 1, 2003; 83(1): 183 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. G. Lloyd and C. D. Hardin
Role of microtubules in the regulation of metabolism in isolated cerebral microvessels
Am J Physiol Cell Physiol, December 1, 1999; 277(6): C1250 - C1262.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online