AJP - Cell Physiology, Vol 268, Issue 4 C869-C876, Copyright © 1995 by American Physiological Society

ARTICLES

Energy-rich phosphates in slow and fast human skeletal muscle

K. Vandenborne, G. Walter, L. Ploutz-Snyder, R. Staron, A. Fry, K. De Meirleir, G. A. Dudley and J. S. Leigh
Department of Rehabilitation Medicine, University of Pennsylvania, Philadelphia 19104, USA.

We investigated the relationship between energy-rich phosphate content and muscle fiber-type composition in human skeletal muscle using a combination of 31P-nuclear magnetic resonance spectroscopy (NMR), histochemical, and biochemical analyses of muscle biopsies. Localized 31P spectra were collected simultaneously from the predominantly slow-twitch soleus muscle and the mixed (fast-twitch and slow-twitch) medial and lateral gastrocnemius muscles, using B1-insensitive Hadamard Spectroscopic Imaging. Biopsy samples were taken from the soleus and lateral gastrocnemius muscles before NMR investigation and analyzed for fiber type composition and succinic dehydrogenase (SDH) activity. Fiber-type composition was determined based both on myofibrillar actomyosin ATPase activity combined with cross-sectional area and on myosin heavy-chain composition. Localized spectroscopy demonstrated a significantly (P < 0.001) higher P(i)/phosphocreatine ratio in the soleus muscle (0.15 +/- 0.01) compared with the medial (0.12 +/- 0.01) and lateral (0.10 +/- 0.0) gastrocnemius. However, in vitro analysis of muscle biopsies showed only a moderate relationship between the basal phosphate content and myofibrillar actomyosin ATPase-based fiber-type composition and SDH activity, respectively.

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