| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 267, Issue 5 C1389-C1397, Copyright © 1994 by American Physiological Society
ARTICLES |
J. Kang, P. Posner and C. Sumners
Department of Physiology, University of Florida, College of Medicine, Gainesville 32610-0274.
Angiotensin II (ANG II) elicits an ANG II type 2 (AT2) receptor-mediated increase in outward K+ current (IK; delayed rectifier K+ current) in neurons cocultured from rat hypothalamus and brain stem. Here we have shown that the AT2-receptor-mediated stimulation of neuronal IK by ANG II (100 nM) was abolished by pretreatment of cultures with pertussis toxin (PTX; 200 ng/ml) and by intracellular application of an antibody against the inhibitory guanine nucleotide (GTP) binding protein (anti-Gi alpha, 1:200). Antibodies against other GTP binding proteins (anti-Go alpha, 1:50 and 1:200; anti-Gq/11 alpha, 1:200) did not alter the AT2-receptor-mediated stimulation of neuronal IK by ANG II (100 nM). Furthermore, this effect of ANG II (100 nM) was inhibited by the serine/threonine phosphatase inhibitor okadaic acid (1-10 nM) and by anti-type 2A protein phosphatase (PP2A) antibodies but not by the tyrosine phosphatase inhibitor sodium orthovanadate (1 mM). Thus we have identified key components (Gi and PP2A) of the signal transduction pathway that is responsible for the AT2-receptor-mediated stimulation of neuronal K+ currents.
This article has been cited by other articles:
|
|
 |
|
  T. Matsuura, C. Sun, L. Leng, A. Kapurniotu, J. Bernhagen, R. Bucala, A. E. Martynyuk, and C. Sumners Macrophage Migration Inhibitory Factor Increases Neuronal Delayed Rectifier K+ Current J Neurophysiol, February 1, 2006; 95(2): 1042 - 1048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Berry, R. Touyz, A. F. Dominiczak, R. C. Webb, and D. G. Johns Angiotensin receptors: signaling, vascular pathophysiology, and interactions with ceramide Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2337 - H2365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hines, J. N. Heerding, S. J. Fluharty, and D. K. Yee Identification of Angiotensin II Type 2 (AT2) Receptor Domains Mediating High-Affinity CGP 42112A Binding and Receptor Activation J. Pharmacol. Exp. Ther., August 1, 2001; 298(2): 665 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhu, C. Sumners, C. H. Gelband, and P. Posner Chronotropic Effect of Angiotensin II via Type 2 Receptors in Rat Brain Neurons J Neurophysiol, May 1, 2001; 85(5): 2177 - 2183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Ferguson, D. L.S. Washburn, and K. J. Latchford Hormonal and Neurotransmitter Roles for Angiotensin in the Regulation of Central Autonomic Function Experimental Biology and Medicine, February 1, 2001; 226(2): 85 - 96. [Abstract] [Full Text]
|
|
|
|
|
|
M. Zhu, R. Natarajan, J. L. Nadler, J. M. Moore, C. H. Gelband, and C. Sumners Angiotensin II Increases Neuronal Delayed Rectifier K+ Current: Role of 12-Lipoxygenase Metabolites of Arachidonic Acid J Neurophysiol, November 1, 2000; 84(5): 2494 - 2501. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mifune, H. Sasamura, R. Shimizu-Hirota, H. Miyazaki, and T. Saruta Angiotensin II Type 2 Receptors Stimulate Collagen Synthesis in Cultured Vascular Smooth Muscle Cells Hypertension, November 1, 2000; 36(5): 845 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Hansen, G. Servant, T. J. Baranski, T. Fujita, T. Iiri, and S. P. Sheikh Functional Reconstitution of the Angiotensin II Type 2 Receptor and Gi Activation Circ. Res., October 27, 2000; 87(9): 753 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. de Gasparo, K. J. Catt, T. Inagami, J. W. Wright, and Th. Unger International Union of Pharmacology. XXIII. The Angiotensin II Receptors Pharmacol. Rev., September 1, 2000; 52(3): 415 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gallinat, S. Busche, M. K. Raizada, and C. Sumners The angiotensin II type 2 receptor: an enigma with multiple variations Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E357 - E374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Herzig and J. Neumann Effects of Serine/Threonine Protein Phosphatases on Ion Channels in Excitable Membranes Physiol Rev, January 1, 2000; 80(1): 173 - 210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Carey, Z.-Q. Wang, and H. M. Siragy Role of the Angiotensin Type 2 Receptor in the Regulation of Blood Pressure and Renal Function Hypertension, January 1, 2000; 35(1): 155 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhu, C. H. Gelband, P. Posner, and C. Sumners Angiotensin II Decreases Neuronal Delayed Rectifier Potassium Current: Role of Calcium/Calmodulin-Dependent Protein Kinase II J Neurophysiol, September 1, 1999; 82(3): 1560 - 1568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Matsubara Pathophysiological Role of Angiotensin II Type 2 Receptor in Cardiovascular and Renal Diseases Circ. Res., December 14, 1998; 83(12): 1182 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tsutsumi, H. Matsubara, N. Ohkubo, Y. Mori, Y. Nozawa, S. Murasawa, K. Kijima, K. Maruyama, H. Masaki, Y. Moriguchi, et al. Angiotensin II Type 2 Receptor Is Upregulated in Human Heart With Interstitial Fibrosis, and Cardiac Fibroblasts Are the Major Cell Type for Its Expression Circ. Res., November 16, 1998; 83(10): 1035 - 1046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Thompson, S. M. Wade, J. K. Harrison, M. N. Salafranca, and R. R. Neubig Cotransfection of Second and Third Intracellular Loop Fragments Inhibit Angiotensin AT1a Receptor Activation of Phospholipase C in HEK-293 Cells J. Pharmacol. Exp. Ther., April 1, 1998; 285(1): 216 - 222. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Zhu, C. H. Gelband, J. M. Moore, P. Posner, and C. Sumners Angiotensin II Type 2 Receptor Stimulation of Neuronal Delayed-Rectifier Potassium Current Involves Phospholipase A2 and Arachidonic Acid J. Neurosci., January 15, 1998; 18(2): 679 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Jacobs and J. G. Douglas Angiotensin II Type 2 Receptor Subtype Mediates Phospholipase A2–Dependent Signaling in Rabbit Proximal Tubular Epithelial Cells Hypertension, October 1, 1996; 28(4): 663 - 668. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Laflamme, M. d. Gasparo, J.-M. Gallo, M. D. Payet, and N. Gallo-Payet Angiotensin II Induction of Neurite Outgrowth by AT2 Receptors in NG108-15 Cells. EFFECT COUNTERACTED BY THE AT1 RECEPTORS J. Biol. Chem., September 13, 1996; 271(37): 22729 - 22735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Hayashida, M. Horiuchi, and V. J. Dzau Intracellular Third Loop Domain of Angiotensin II Type-2 Receptor. ROLE IN MEDIATING SIGNAL TRANSDUCTION AND CELLULAR FUNCTION J. Biol. Chem., September 6, 1996; 271(36): 21985 - 21992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Martens, D. Wang, C. Sumners, P. Posner, and C. H. Gelband Angiotensin II Type 2 Receptor–Mediated Regulation of Rat Neuronal K+ Channels Circ. Res., August 1, 1996; 79(2): 302 - 309. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ichiki, Y. Kambayashi, and T. Inagami Multiple Growth Factors Modulate mRNA Expression of Angiotensin II Type-2 Receptor in R3T3 Cells Circ. Res., December 1, 1995; 77(6): 1070 - 1076. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
