Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 267: C1288-C1294, 1994;
0363-6143/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kusaka, M.
Right arrow Articles by Sperelakis, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kusaka, M.
Right arrow Articles by Sperelakis, N.

AJP - Cell Physiology, Vol 267, Issue 5 C1288-C1294, Copyright © 1994 by American Physiological Society

ARTICLES

Fast sodium currents induced by serum in human uterine leiomyosarcoma cells

M. Kusaka and N. Sperelakis
Department of Molecular and Cellular Physiology, College of Medicine, University of Cincinnati, Ohio 45267.

We previously demonstrated that fast Na+ channels are expressed in uterine smooth muscle cells during pregnancy [Y. Ohya, and N. Sperelakis. Am. J. Physiol. 257 (Cell Physiol. 26): C408-C412, 1989; Y. Inoue, and N. Sperelakis. Am. J. Physiol. 260 (Cell Physiol. 29): C658-C663, 1991]. In the present study, we investigated the possible expression of fast Na+ channels and resultant fast Na+ current [INa(f)] using whole cell voltage clamp in cultured uterine leiomyosarcoma cells (SK-UT-1B), which are derived from human myometrium. The expression of INa(f) was dependent on the concentration of fetal bovine serum (FBS) in the culture medium. The mean current densities of peak INa(f) in cells incubated with 0, 1, 5, and 10% FBS were (in pA/pF) 1.3 +/- 0.5, 1.6 +/- 0.9, 9.8 +/- 2.4, and 9.8 +/- 1.7, respectively. Two types of INa(f) were identified based on different time courses of current decay. Both types of current were equally tetrodotoxin sensitive (half-maximal inhibitory concentration approximately 50 nM) and had similar electrical properties. These characteristics are consistent with those in the pregnant myometrial cells, yet the current density in the sarcoma cells is much higher. These findings suggest that one or more factors in serum induce INa(f) in the uterine sarcoma cells, and this cell line could be a good model for the study of the fast Na+ channels in uterine smooth muscle.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Gosling, S. L. Harley, R. J. Turner, N. Carey, and J. T. Powell
Human Saphenous Vein Endothelial Cells Express a Tetrodotoxin-resistant, Voltage-gated Sodium Current
J. Biol. Chem., August 14, 1998; 273(33): 21084 - 21090.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
H. KURIYAMA, K. KITAMURA, T. ITOH, and R. INOUE
Physiological Features of Visceral Smooth Muscle Cells, With Special Reference to Receptors and Ion Channels
Physiol Rev, July 1, 1998; 78(3): 811 - 920.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Gomez and K. Sward
Long-term regulation of contractility and calcium current in smooth muscle
Am J Physiol Cell Physiol, November 1, 1997; 273(5): C1714 - C1720.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online