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Am J Physiol Cell Physiol 267: C1231-C1238, 1994;
0363-6143/94 $5.00
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AJP - Cell Physiology, Vol 267, Issue 5 C1231-C1238, Copyright © 1994 by American Physiological Society

ARTICLES

Cloning and characterization of a Kv1.5 delayed rectifier K+ channel from vascular and visceral smooth muscles

K. E. Overturf, S. N. Russell, A. Carl, F. Vogalis, P. J. Hart, J. R. Hume, K. M. Sanders and B. Horowitz
Department of Physiology, University of Nevada School of Medicine, Reno 89557.

We have cloned and characterized the expression of a Kv1.5 K+ channel (cKv1.5) from canine colonic smooth muscle. The amino acid sequence displayed a high level of identity to other K+ channels of the Kv1.5 class in the core region between transmembrane segments S1-S6; however, identity decreased to between 74 and 82% in the NH2 and COOH terminal segments, suggesting that cKv1.5 is a distinct isoform of the Kv1.5 class. Functional expression of cKv1.5 in oocytes demonstrated a channel highly selective for K+, which activates in a voltage-dependent manner on depolarization to membrane potentials positive to -40 mV. At room temperature the channel showed fast activation (time to half of peak current, 5.5 ms) and slow inactivation that was incomplete after 20-s depolarizations. Single channel analysis of the channel expressed in oocytes displayed a linear current-voltage curve and had a slope conductance of 9.8 +/- 1.1 pS. Northern blot analysis demonstrated differential expression of cKv1.5 in smooth muscles of the gastrointestinal tract and abundant expression in several vascular smooth muscles. We propose that cKv1.5 represents a component of the delayed rectifier current in both vascular and visceral smooth muscles.


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