| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 267, Issue 4 C901-C908, Copyright © 1994 by American Physiological Society
ARTICLES |
A. A. McDonough, C. E. Magyar and Y. Komatsu
Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033.
The activity of Na(+)-K(+)-adenosinetriphosphatase (Na(+)-K(+)-ATPase), the sodium pump, which drives active Na+ reabsorption along the nephron, varies over an order of magnitude, depending on the nephron segment, and activity is increased in the outer medullary collecting tubule (MCT) during hypokalemia. The aims of the present study were to assess abundance of sodium pump alpha 1- and beta 1-subunits in dissected nephron segments of the rat by immunoblotting, to determine if alpha 2- or alpha 3-protein could be detected in the collecting tubules, as suggested by Barlet-Bas et al. (C. Barlet-Bas, E. Arystarkhova, L. Cheval, S. Marsy, K. Sweadner, N. Modyanov, and A. Doucet. J. Biol. Chem. 268: 11512-11515, 1993) for rabbit, and to determine if alpha 1 and beta 1 were increased in MCT by hypokalemia. Tubules from the rat were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (12-100 mm/lane), blotted, and probed with subunit-specific antisera. alpha 1 and beta 1, detected in all tubule segments assayed, were highest in cortical and medullary thick ascending limbs and proximal convoluted tubule (PCT), lower in the MCT and barely detectable in proximal straight tubule. In the cortical collecting tubule (CCT), alpha 1 abundance was equivalent to that in PCT, whereas beta 1 and enzymatic activity were both less than one-half of that in PCT. After 2 wk of a K(+)-deficient diet, alpha 1- and beta 1-subunit levels in MCT increased 3.4 +/- 0.6- and 11.7 +/- 4.0-fold, respectively, associated with a 5-fold increase in activity. alpha 2 and alpha 3 were not detected in the CCT or MCT.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
 |
|
  L. E. Yang, M. B. Sandberg, A. D. Can, K. Pihakaski-Maunsbach, and A. A. McDonough Effects of dietary salt on renal Na+ transporter subcellular distribution, abundance, and phosphorylation status Am J Physiol Renal Physiol, October 1, 2008; 295(4): F1003 - F1016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yu, B. Lopez, E. A. Dos Santos, J. R. Falck, and R. J. Roman Effects of 20-HETE on Na+ transport and Na+-K+-ATPase activity in the thick ascending loop of Henle Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2007; 292(6): R2400 - R2405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Kaunitz Membrane transport proteins: not just for transport anymore Am J Physiol Renal Physiol, May 1, 2006; 290(5): F995 - F996. [Full Text] [PDF]
|
|
|
|
|
|
J. Lebowitz, B. An, R. S. Edinger, M. L. Zeidel, and J. P. Johnson Effect of altered Na+ entry on expression of apical and basolateral transport proteins in A6 epithelia Am J Physiol Renal Physiol, September 1, 2003; 285(3): F524 - F531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhou, W. Yin, S. Q. Doi, S. W. Robinson, K. Takeyasu, and X. Fan Stimulation of Na,K-ATPase by low potassium requires reactive oxygen species Am J Physiol Cell Physiol, August 1, 2003; 285(2): C319 - C326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Wetzel and K. J. Sweadner Phospholemman expression in extraglomerular mesangium and afferent arteriole of the juxtaglomerular apparatus Am J Physiol Renal Physiol, July 1, 2003; 285(1): F121 - F129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Yang, P. K. K. Leong, S. Ye, V. M. Campese, and A. A. McDonough Responses of proximal tubule sodium transporters to acute injury-induced hypertension Am J Physiol Renal Physiol, February 1, 2003; 284(2): F313 - F322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. McDonough, C. B. Thompson, and J. H. Youn Skeletal muscle regulates extracellular potassium Am J Physiol Renal Physiol, June 1, 2002; 282(6): F967 - F974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. MAGYAR, Y. ZHANG, N.-H. HOLSTEIN-RATHLOU, and A. A. MCDONOUGH Downstream Shift in Sodium Pump Activity along the Nephron during Acute Hypertension J. Am. Soc. Nephrol., November 1, 2001; 12(11): 2231 - 2240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Muller-Ehmsen, P. Juvvadi, C. B. Thompson, L. Tumyan, M. Croyle, J. B. Lingrel, R. H. G. Schwinger, A. A. McDonough, and R. A. Farley Ouabain and substrate affinities of human Na+-K+-ATPase alpha 1beta 1, alpha 2beta 1, and alpha 3beta 1 when expressed separately in yeast cells Am J Physiol Cell Physiol, October 1, 2001; 281(4): C1355 - C1364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Wetzel and K. J. Sweadner Immunocytochemical localization of Na-K-ATPase alpha - and gamma -subunits in rat kidney Am J Physiol Renal Physiol, September 1, 2001; 281(3): F531 - F545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Muto Potassium Transport in the Mammalian Collecting Duct Physiol Rev, January 1, 2001; 81(1): 85 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D'Cotta, C. Valotaire, F. le Gac, and P. Prunet Synthesis of gill Na+-K+-ATPase in Atlantic salmon smolts: differences in alpha -mRNA and alpha -protein levels Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2000; 278(1): R101 - R110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Arystarkhova, R. K. Wetzel, N. K. Asinovski, and K. J. Sweadner The gamma Subunit Modulates Na+ and K+ Affinity of the Renal Na,K-ATPase J. Biol. Chem., November 19, 1999; 274(47): 33183 - 33185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Féraille, M. L. Carranza, S. Gonin, P. Béguin, C. Pedemonte, M. Rousselot, J. Caverzasio, K. Geering, P.-Y. Martin, and H. Favre Insulin-induced Stimulation of Na+,K+-ATPase Activity in Kidney Proximal Tubule Cells Depends on Phosphorylation of the alpha -Subunit at Tyr-10 Mol. Biol. Cell, September 1, 1999; 10(9): 2847 - 2859. [Abstract] [Full Text]
|
|
|
|
|
|
G.-H. KIM, C. ECELBARGER, M. A. KNEPPER, and R. K. PACKER Regulation of Thick Ascending Limb Ion Transporter Abundance in Response to Altered Acid/Base Intake J. Am. Soc. Nephrol., May 1, 1999; 10(5): 935 - 942. [Abstract] [Full Text]
|
|
|
|
|
|
G. Blanco and R. W. Mercer Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function Am J Physiol Renal Physiol, November 1, 1998; 275(5): F633 - F650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mahaney, C. Felton, D. Taylor, W. Fleming, J. Q. Kong, and C. Baylis Renal cortical Na+-K+-ATPase activity and abundance is decreased in normal pregnant rats Am J Physiol Renal Physiol, November 1, 1998; 275(5): F812 - F817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Codina, J. T. Delmas-Mata, and T. D. DuBose Jr. Expression of HKalpha 2 protein is increased selectively in renal medulla by chronic hypokalemia Am J Physiol Renal Physiol, September 1, 1998; 275(3): F433 - F440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Schafer, M. L. Watkins, L. Li, P. Herter, S. Haxelmans, and E. Schlatter A simplified method for isolation of large numbers of defined nephron segments Am J Physiol Renal Physiol, October 1, 1997; 273(4): F650 - F657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Bundgaard, T. A. Schmidt, J. S. Larsen, and K. Kjeldsen K+ supplementation increases muscle [Na+-K+-ATPase] and improves extrarenal K+ homeostasis in rats J Appl Physiol, April 1, 1997; 82(4): 1136 - 1144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Wall, M. P. Fischer, G.-H. Kim, B.-M. Nguyen, and K. A. Hassell In rat inner medullary collecting duct, NH+4 uptake by the Na,K-ATPase is increased during hypokalemia Am J Physiol Renal Physiol, January 1, 2002; 282(1): F91 - F102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Scherzer and M. M. Popovtzer Segmental localization of mRNAs encoding Na+-K+-ATPase alpha 1- and beta 1-subunits in diabetic rat kidneys using RT-PCR Am J Physiol Renal Physiol, March 1, 2002; 282(3): F492 - F500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Arystarkhova, R. K. Wetzel, and K. J. Sweadner Distribution and oligomeric association of splice forms of Na+-K+-ATPase regulatory gamma -subunit in rat kidney Am J Physiol Renal Physiol, March 1, 2002; 282(3): F393 - F407. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
