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Am J Physiol Cell Physiol 267: C821-C826, 1994;
0363-6143/94 $5.00
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AJP - Cell Physiology, Vol 267, Issue 3 C821-C826, Copyright © 1994 by American Physiological Society

ARTICLES

Anti-idiotypic antibodies to delineate epitope specificity of anti-amiloride antibodies

C. Lin, M. Musch, P. Meo, J. Zebrowitz, E. Chang and T. R. Kleyman
Department of Medicine, University of Pennsylvania, Philadelphia.

Amiloride and related compounds have found widespread use as cation transport inhibitors. We have previously raised a series of polyclonal anti-amiloride antibodies using different amiloride-protein conjugates as immunogens, where amiloride was coupled to protein either through its guanidino moiety or through its 5-aminopyrazinyl moiety. The anti-amiloride antibodies recognized distinct sites on amiloride, and the site of attachment of amiloride to carrier protein was a critical factor in determining which part of the amiloride molecule was recognized by the anti-amiloride antibody. The specificity of binding of amiloride analogues to these polyclonal anti-amiloride antibodies mimicked the specificity of binding of amiloride analogues to selected isoforms of the epithelial Na+ channel or the Na+/H+ exchanger, suggesting that antigen binding site of these antibodies might be similar in structure to amiloride binding sites on selected Na+ transport proteins. We previously generated monoclonal anti-idiotypic antibodies RA2.4 and RA6.3 by an auto-anti-idiotypic approach, using amiloride coupled to albumin through the guanidinium moiety (amiloride-A1). We have now raised a series of monoclonal anti-idiotypic antibodies, T6, T26, T40, and T181, using amiloride coupled to keyhole limpet hemocyanin through the 5-aminopyrazinyl moiety (amiloride-A5) as an immunogen with the same auto-anti-idiotypic approach. These monoclonal anti-idiotypic antibodies recognized both polyclonal anti-amiloride-A1 and anti-amiloride-A5 antibodies, suggesting that idiotype-anti-idiotype interaction was not epitope restricted.(ABSTRACT TRUNCATED AT 250 WORDS)


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O. Kretz, P. Barbry, R. Bock, and B. Lindemann
Differential Expression of RNA and Protein of the Three Pore-forming Subunits of the Amiloride-sensitive Epithelial Sodium Channel in Taste Buds of the Rat
J. Histochem. Cytochem., January 1, 1999; 47(1): 51 - 64.
[Abstract] [Full Text]


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