| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 267, Issue 3 C796-C803, Copyright © 1994 by American Physiological Society
ARTICLES |
J. H. Cho, M. W. Musch, A. M. DePaoli, C. M. Bookstein, Y. Xie, C. F. Burant, M. C. Rao and E. B. Chang
Department of Medicine, University of Chicago 60637.
Na+/H+ exchangers (NHEs) are integral membrane proteins that exchange Na+ for H+ across membranes. Four isoforms have been cloned (NHE-1-4). NHE-3 localizes to the apical domain, and its expression is increased in dexamethasone-treated rats by Northern analysis. This stimulatory effect on expression is region and tissue specific, being present in ileum and proximal colon, but not in jejunum, distal colon, or kidney. The increase in transcript expression in ileum correlates with an increase in protein expression by immunoblotting. Changes in apical Na+/H+ exchange activity, as measured by 22Na uptake into brush-border membrane vesicles, correlate with expression differences, with significant increases observed in ileum and proximal colon. In situ hybridization showed NHE-3 mRNA only in villus and absorptive cells of control rats, the pattern not being altered by dexamethasone treatment. This suggests that dexamethasone does not increase expression by inducing crypt cells to express NHE-3 prematurely. We conclude that glucocorticoids selectively increase intestinal NHE-3 activity in a region-specific manner and that this effect also appears to be tissue specific.
This article has been cited by other articles:
|
|
 |
|
  M. W. Musch, A. Lucioni, and E. B. Chang Aldosterone regulation of intestinal Na absorption involves SGK-mediated changes in NHE3 and Na+ pump activity Am J Physiol Gastrointest Liver Physiol, November 1, 2008; 295(5): G909 - G919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Donowitz and X. Li Regulatory Binding Partners and Complexes of NHE3 Physiol Rev, July 1, 2007; 87(3): 825 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wang, H. Zhang, F. Lang, and C. C. Yun Acute activation of NHE3 by dexamethasone correlates with activation of SGK1 and requires a functional glucocorticoid receptor Am J Physiol Cell Physiol, January 1, 2007; 292(1): C396 - C404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. A. Bobulescu, V. Dwarakanath, L. Zou, J. Zhang, M. Baum, and O. W. Moe Glucocorticoids acutely increase cell surface Na+/H+ exchanger-3 (NHE3) by activation of NHE3 exocytosis Am J Physiol Renal Physiol, October 1, 2005; 289(4): F685 - F691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Musch, C. Bookstein, F. Rocha, A. Lucioni, H. Ren, J. Daniel, Y. Xie, R. L. McSwine, M. C. Rao, J. Alverdy, et al. Region-specific adaptation of apical Na/H exchangers after extensive proximal small bowel resection Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G975 - G985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. B. Renes, M. Verburg, D. J. P. M. Van Nispen, H. A. Buller, J. Dekker, and A. W. C. Einerhand Distinct epithelial responses in experimental colitis: implications for ion uptake and mucosal protection Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G169 - G179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lucioni, C. Womack, M. W. Musch, F. L. Rocha, C. Bookstein, and E. B. Chang Metabolic acidosis in rats increases intestinal NHE2 and NHE3 expression and function Am J Physiol Gastrointest Liver Physiol, July 1, 2002; 283(1): G51 - G56. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Yun, Y. Chen, and F. Lang Glucocorticoid Activation of Na+/H+ Exchanger Isoform 3 Revisited. THE ROLES OF SGK1 AND NHERF2 J. Biol. Chem., March 1, 2002; 277(10): 7676 - 7683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Blikslager, E. Hunt, R. Guerrant, M. Rhoads, and R. Argenzio Glutamine transporter in crypts compensates for loss of villus absorption in bovine cryptosporidiosis Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G645 - G653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xu, J. F. Collins, L. Bai, P. R. Kiela, R. M. Lynch, and F. K. Ghishan Epidermal growth factor regulation of rat NHE2 gene expression Am J Physiol Cell Physiol, August 1, 2001; 281(2): C504 - C513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Rocha, M. W. Musch, L. Lishanskiy, C. Bookstein, K. Sugi, Y. Xie, and E. B. Chang IFN-{gamma} downregulates expression of Na+/H+ exchangers NHE2 and NHE3 in rat intestine and human Caco-2/bbe cells Am J Physiol Cell Physiol, May 1, 2001; 280(5): C1224 - C1232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Musch, C. Bookstein, Y. Xie, J. H. Sellin, and E. B. Chang SCFA increase intestinal Na absorption by induction of NHE3 in rat colon and human intestinal C2/bbe cells Am J Physiol Gastrointest Liver Physiol, April 1, 2001; 280(4): G687 - G693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Malakooti, R. Y. Dahdal, P. K. Dudeja, T. J. Layden, and K. Ramaswamy The human Na+/H+ exchanger NHE2 gene: genomic organization and promoter characterization Am J Physiol Gastrointest Liver Physiol, April 1, 2001; 280(4): G763 - G773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Kiela, Y. S. Guner, H. Xu, J. F. Collins, and F. K. Ghishan Age- and tissue-specific induction of NHE3 by glucocorticoids in the rat small intestine Am J Physiol Cell Physiol, April 1, 2000; 278(4): C629 - C637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Cavet, S. Akhter, F. S. de Medina, M. Donowitz, and C.-M. Tse Na+/H+ exchangers (NHE1-3) have similar turnover numbers but different percentages on the cell surface Am J Physiol Cell Physiol, December 1, 1999; 277(6): C1111 - C1121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. S. Guner, P. R. Kiela, H. Xu, J. F. Collins, and F. K. Ghishan Differential regulation of renal sodium-phosphate transporter by glucocorticoids during rat ontogeny Am J Physiol Cell Physiol, November 1, 1999; 277(5): C884 - C890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ikuma, M. Kashgarian, H. J. Binder, and V. M. Rajendran Differential regulation of NHE isoforms by sodium depletion in proximal and distal segments of rat colon Am J Physiol Gastrointest Liver Physiol, February 1, 1999; 276(2): G539 - G549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Khan, F. M. Al-Awadi, and H. Abul Colitis-Induced Changes in the Expression of the Na+/H+ Exchanger Isoform NHE-1 J. Pharmacol. Exp. Ther., May 1, 1998; 285(2): 869 - 875. [Abstract] [Full Text]
|
|
|
|
|
|
L. Wormmeester, F. S. De Medina, F. Kokke, C.-M. Tse, S. Khurana, J. Bowser, M. E. Cohen, and M. Donowitz Quantitative contribution of NHE2 and NHE3 to rabbit ileal brush-border Na+/H+ exchange Am J Physiol Cell Physiol, May 1, 1998; 274(5): C1261 - C1272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Cho, M. W. Musch, C. M. Bookstein, R. L. McSwine, K. Rabenau, and E. B. Chang Aldosterone stimulates intestinal Na+ absorption in rats by increasing NHE3 expression of the proximal colon Am J Physiol Cell Physiol, March 1, 1998; 274(3): C586 - C594. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
