Am J Physiol Cell Physiol Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 267: C784-C795, 1994;
0363-6143/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, J.
Right arrow Articles by Mandel, L. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, J.
Right arrow Articles by Mandel, L. J.

AJP - Cell Physiology, Vol 267, Issue 3 C784-C795, Copyright © 1994 by American Physiological Society

ARTICLES

Cytoskeletal dissociation of ezrin during renal anoxia: role in microvillar injury

J. Chen, R. B. Doctor and L. J. Mandel
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710.

The association/dissociation of ezrin, a microvillar membrane-cytoskeleton linker, was studied to search for the initial step leading to anoxia-induced brush-border breakdown in a rabbit proximal tubule suspension. Electron microscopy studies display time-dependent damage to the microvilli during anoxia; immunoblots demonstrate the dissociation of ezrin from the cytoskeleton, reflected by the significant decrease in Triton X-100-insoluble ezrin from control (91%) to 39% after 30 min. Simultaneously, Triton X-100-soluble and extracellular ezrin increased with no change in total ezrin, Triton X-100 solubility of actin, or total intracellular protein. Parallel immunocytochemistry studies show diffusion of ezrin from the brush border, where ezrin is highly colocalized with F-actin during normoxia into the cytoplasm. Thirty minutes of reoxygenation following 30 min of anoxia causes recovery of the microvillar structure and reassociation of ezrin to the cytoskeleton and the brush border. Application of ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (4 mM) or inhibition of intracellular calpain or calcineurin do not prevent the dissociation of ezrin during anoxia. We conclude that ezrin-cytoskeletal dissociation may initiate microvillar breakdown during anoxia via calcium-independent mechanisms.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Zhu, J. Hatakeyama, C. Chen, A. Shastri, K. Poon, and J. G. Forte
Comparative study of ezrin phosphorylation among different tissues: more is good; too much is bad
Am J Physiol Cell Physiol, July 1, 2008; 295(1): C192 - C202.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. A. Hallett, P. C. Dagher, and S. J. Atkinson
Rho GTPases show differential sensitivity to nucleotide triphosphate depletion in a model of ischemic cell injury
Am J Physiol Cell Physiol, July 1, 2003; 285(1): C129 - C138.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
T. GRUNE, T. REINHECKEL, J. A. NORTH, R. LI, P. B. BESCOS, R. SHRINGARPURE, and K. J. A. DAVIES
Ezrin turnover and cell shape changes catalyzed by proteasome in oxidatively stressed cells
FASEB J, October 1, 2002; 16(12): 1602 - 1610.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
V. R. Price, C. A. Reed, W. Lieberthal, and J. H. Schwartz
ATP Depletion Of Tubular Cells Causes Dissociation of the Zonula Adherens and Nuclear Translocation of {beta}-Catenin and LEF-1
J. Am. Soc. Nephrol., May 1, 2002; 13(5): 1152 - 1161.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Boyd-White, A. Srirangam, M. P. Goheen, and M. C. Wagner
Ischemia disrupts myosin Ibeta in renal tubules
Am J Physiol Cell Physiol, October 1, 2001; 281(4): C1326 - C1335.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. L. Ashworth, R. M. Sandoval, M. Hosford, J. R. Bamburg, and B. A. Molitoris
Ischemic injury induces ADF relocalization to the apical domain of rat proximal tubule cells
Am J Physiol Renal Physiol, May 1, 2001; 280(5): F886 - F894.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Chen and M. C. Wagner
Altered membrane-cytoskeleton linkage and membrane blebbing in energy-depleted renal proximal tubular cells
Am J Physiol Renal Physiol, April 1, 2001; 280(4): F619 - F627.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. Gautreau, D. Louvard, and M. Arpin
Morphogenic Effects of Ezrin Require a Phosphorylation-induced Transition from Oligomers to Monomers at the Plasma Membrane
J. Cell Biol., July 11, 2000; 150(1): 193 - 204.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. White, R. B. Doctor, R. H. Dahl, and J. Chen
Coincident microvillar actin bundle disruption and perinuclear actin sequestration in anoxic proximal tubule
Am J Physiol Renal Physiol, June 1, 2000; 278(6): F886 - F893.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Chen and L. J. Mandel
Role of water and electrolyte influxes in anoxic plasma membrane disruption
Am J Physiol Cell Physiol, October 1, 1997; 273(4): C1341 - C1348.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Roy, M. Martin, and P. Mangeat
A Dual Involvement of the Amino-terminal Domain of Ezrin in F- and G-actin Binding
J. Biol. Chem., August 8, 1997; 272(32): 20088 - 20095.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online