| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 267, Issue 3 C753-C758, Copyright © 1994 by American Physiological Society
ARTICLES |
M. J. Kuchan, H. Jo and J. A. Frangos
Department of Chemical Engineering, Pennsylvania State University, University Park 16802.
Exposure of cultured endothelial cells to shear stress resulting from well-defined fluid flow stimulates the production of nitric oxide (NO). We have established that an initial burst in production is followed by sustained steady-state NO production. The signal transduction events leading to this stimulation are not well understood. In the present study, we examined the role of regulatory guanine nucleotide binding proteins (G proteins) in shear stress-mediated NO production. In endothelial cells not exposed to shear stress, AIF4-, a general activator of G proteins, markedly elevated the production of guanosine 3',5'-cyclic monophosphate (cGMP). Pretreatment with NO synthase inhibitor N omega-nitro-L-arginine completely blocked this stimulation. Incubation with guanosine 5'-O-(2-thiodiphosphate) (GDP beta S), a general G protein inhibitor, blocked the flow-mediated burst in cGMP production in a dose-dependent manner. Likewise, GDP beta S inhibited NOx (NO2 + NO3) production for the 1st h. However, inhibition was not detectable between 1 and 3 h. Pertussis toxin (PTx) had no effect on the shear response at any time point. The burst in NO production caused by a change in shear stress appears to be dependent on a PTx-refractory G protein. Sustained shear-mediated production is independent of G protein activation.
This article has been cited by other articles:
|
|
 |
|
  L. A. Otte, K. S. Bell, L. Loufrani, J.-C. Yeh, B.ît Melchior, D. N. Dao, H. Y. Stevens, C. R. White, and J. A. Frangos Rapid changes in shear stress induce dissociation of a G\#945;q/11\#8211;platelet endothelial cell adhesion molecule-1 complex J. Physiol., May 15, 2009; 587(10): 2365 - 2373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Chu and D. G. Peters Serial analysis of the vascular endothelial transcriptome under static and shear stress conditions Physiol Genomics, July 1, 2008; 34(2): 185 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Chen, M. I. Latz, P. Sobolewski, and J. A. Frangos Evidence for the role of G-proteins in flow stimulation of dinoflagellate bioluminescence Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R2020 - R2027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chien Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1209 - H1224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kurita, T. Matsui, T. Ishizuka, B. Takase, and K. Satomura Significance of Plasma Nitric Oxide/Endothelial-1 Ratio for Prediction of Coronary Artery Disease Angiology, May 1, 2005; 56(3): 259 - 264. [Abstract] [PDF]
|
|
|
|
|
|
R. D. Shipley, S. J. Kim, and J. M. Muller-Delp Time course of flow-induced vasodilation in skeletal muscle: contributions of dilator and constrictor mechanisms Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1499 - H1507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Radel and V. Rizzo Integrin mechanotransduction stimulates caveolin-1 phosphorylation and recruitment of Csk to mediate actin reorganization Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H936 - H945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Cabrales, A. G. Tsai, and M. Intaglietta Microvascular pressure and functional capillary density in extreme hemodilution with low- and high-viscosity dextran and a low-viscosity Hb-based O2 carrier Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H363 - H373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. K. Baskurt, O. Yalcin, S. Ozdem, J. K. Armstrong, and H. J. Meiselman Modulation of endothelial nitric oxide synthase expression by red blood cell aggregation Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H222 - H229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. C. Boo and H. Jo Flow-dependent regulation of endothelial nitric oxide synthase: role of protein kinases Am J Physiol Cell Physiol, September 1, 2003; 285(3): C499 - C508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mochizuki, H. Vink, O. Hiramatsu, T. Kajita, F. Shigeto, J. A. E. Spaan, and F. Kajiya Role of hyaluronic acid glycosaminoglycans in shear-induced endothelium-derived nitric oxide release Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H722 - H726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wedgwood, C. J. Mitchell, J. R. Fineman, and S. M. Black Developmental differences in the shear stress-induced expression of endothelial NO synthase: changing role of AP-1 Am J Physiol Lung Cell Mol Physiol, April 1, 2003; 284(4): L650 - L662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Czarny, J. Liu, P. Oh, and J. E. Schnitzer Transient Mechanoactivation of Neutral Sphingomyelinase in Caveolae to Generate Ceramide J. Biol. Chem., February 7, 2003; 278(7): 4424 - 4430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Peters, X.-C. Zhang, P. V. Benos, E. Heidrich-O'Hare, and R. E. Ferrell Genomic analysis of immediate/early response to shear stress in human coronary artery endothelial cells Physiol Genomics, December 26, 2002; 12(1): 25 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. C. Boo, J. Hwang, M. Sykes, B. J. Michell, B. E. Kemp, H. Lum, and H. Jo Shear stress stimulates phosphorylation of eNOS at Ser635 by a protein kinase A-dependent mechanism Am J Physiol Heart Circ Physiol, November 1, 2002; 283(5): H1819 - H1828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-M. Go, Y. C. Boo, H. Park, M. C. Maland, R. Patel, K. A. Pritchard Jr., Y. Fujio, K. Walsh, V. Darley-Usmar, and H. Jo Protein kinase B/Akt activates c-Jun NH2-terminal kinase by increasing NO production in response to shear stress J Appl Physiol, October 1, 2001; 91(4): 1574 - 1581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wedgwood, J. M. Bekker, and S. M. Black Shear stress regulation of endothelial NOS in fetal pulmonary arterial endothelial cells involves PKC Am J Physiol Lung Cell Mol Physiol, August 1, 2001; 281(2): L490 - L498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Bao, C. Lu, and J. A. Frangos Mechanism of temporal gradients in shear-induced ERK1/2 activation and proliferation in endothelial cells Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H22 - H29. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Hampl and J. Herget Role of Nitric Oxide in the Pathogenesis of Chronic Pulmonary Hypertension Physiol Rev, October 1, 2000; 80(4): 1337 - 1372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. X. Chen, K. D. Ryder, F. M. Pavalko, C. H. Turner, D. B. Burr, J. Qiu, and R. L. Duncan Ca2+ regulates fluid shear-induced cytoskeletal reorganization and gene expression in osteoblasts Am J Physiol Cell Physiol, May 1, 2000; 278(5): C989 - C997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakano, R. Tominaga, I. Nagano, H. Okabe, and H. Yasui Pulsatile flow enhances endothelium-derived nitric oxide release in the peripheral vasculature Am J Physiol Heart Circ Physiol, April 1, 2000; 278(4): H1098 - H1104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Haidekker, N. L'Heureux, and J. A. Frangos Fluid shear stress increases membrane fluidity in endothelial cells: a study with DCVJ fluorescence Am J Physiol Heart Circ Physiol, April 1, 2000; 278(4): H1401 - H1406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kano, K. Katoh, and K. Fujiwara Lateral Zone of Cell-Cell Adhesion as the Major Fluid Shear Stress-Related Signal Transduction Site Circ. Res., March 3, 2000; 86(4): 425 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Osanai, N. Fujita, N. Fujiwara, T. Nakano, K. Takahashi, W. Guan, and K. Okumura Cross talk of shear-induced production of prostacyclin and nitric oxide in endothelial cells Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H233 - H238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Go, R. P. Patel, M. C. Maland, H. Park, J. S. Beckman, V. M. Darley-Usmar, and H. Jo Evidence for peroxynitrite as a signaling molecule in flow-dependent activation of c-Jun NH2-terminal kinase Am J Physiol Heart Circ Physiol, October 1, 1999; 277(4): H1647 - H1653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. A. Majid, K. E. Said, and S. A. Omoro Responses to Acute Changes in Arterial Pressure on Renal Medullary Nitric Oxide Activity in Dogs Hypertension, October 1, 1999; 34(4): 832 - 836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Chiu, B. S. Wung, H. J. Hsieh, L. W. Lo, and D. L. Wang Nitric Oxide Regulates Shear Stress–Induced Early Growth Response-1 : Expression via the Extracellular Signal–Regulated Kinase Pathway in Endothelial Cells Circ. Res., August 6, 1999; 85(3): 238 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Patel, N. Hogg, N. Y. Spencer, B. Kalyanaraman, S. Matalon, and V. M. Darley-Usmar Biochemical Characterization of Human S-Nitrosohemoglobin. EFFECTS ON OXYGEN BINDING AND TRANSNITROSATION J. Biol. Chem., May 28, 1999; 274(22): 15487 - 15492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-H. Yeh, Y. J. Park, R. J. Hansalia, I. S. Ahmed, S. S. Deshpande, P. J. Goldschmidt-Clermont, K. Irani, and B. R. Alevriadou Shear-induced tyrosine phosphorylation in endothelial cells requires Rac1-dependent production of ROS Am J Physiol Cell Physiol, April 1, 1999; 276(4): C838 - C847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Rizzo, D. P. McIntosh, P. Oh, and J. E. Schnitzer In Situ Flow Activates Endothelial Nitric Oxide Synthase in Luminal Caveolae of Endothelium with Rapid Caveolin Dissociation and Calmodulin Association J. Biol. Chem., December 25, 1998; 273(52): 34724 - 34729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Park, Y.-M. Go, P. L. St. John, M. C. Maland, M. P. Lisanti, D. R. Abrahamson, and H. Jo Plasma Membrane Cholesterol Is a Key Molecule in Shear Stress-dependent Activation of Extracellular Signal-regulated Kinase J. Biol. Chem., November 27, 1998; 273(48): 32304 - 32311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Black, J. R. Fineman, R. H. Steinhorn, J. Bristow, and S. J. Soifer Increased endothelial NOS in lambs with increased pulmonary blood flow and pulmonary hypertension Am J Physiol Heart Circ Physiol, November 1, 1998; 275(5): H1643 - H1651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Go, H. Park, M. C. Maland, V. M. Darley-Usmar, B. Stoyanov, R. Wetzker, and H. Jo Phosphatidylinositol 3-kinase gamma mediates shear stress-dependent activation of JNK in endothelial cells Am J Physiol Heart Circ Physiol, November 1, 1998; 275(5): H1898 - H1904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. T. Nowicki Effects of sustained flow reduction on postnatal intestinal circulation Am J Physiol Gastrointest Liver Physiol, October 1, 1998; 275(4): G758 - G768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Redmond, P. A. Cahill, and J. V. Sitzmann Flow-Mediated Regulation of G-Protein Expression in Cocultured Vascular Smooth Muscle and Endothelial Cells Arterioscler Thromb Vasc Biol, January 1, 1998; 18(1): 75 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.J. Chiu, B.S. Wung, J. Y.J. Shyy, H.J. Hsieh, and D.L. Wang Reactive Oxygen Species Are Involved in Shear Stress-Induced Intercellular Adhesion Molecule-1 Expression in Endothelial Cells Arterioscler Thromb Vasc Biol, December 1, 1997; 17(12): 3570 - 3577. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Moazzam, F. A. DeLano, B. W. Zweifach, and G. W. Schmid-Schonbein The leukocyte response to fluid stress PNAS, May 13, 1997; 94(10): 5338 - 5343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Terada, J. E. Repine, D. Piermattei, and B. M. Hybertson Endogenous nitric oxide decreases xanthine oxidase-mediated neutrophil adherence: role of P-selectin J Appl Physiol, March 1, 1997; 82(3): 913 - 917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jo, K. Sipos, Y.-M. Go, R. Law, J. Rong, and J. M. McDonald Differential Effect of Shear Stress on Extracellular Signal-regulated Kinase and N-terminal Jun Kinase in Endothelial Cells. Gi2- AND Gbeta /gamma -DEPENDENT SIGNALING PATHWAYS J. Biol. Chem., January 10, 1997; 272(2): 1395 - 1401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kano, K. Katoh, M. Masuda, and K. Fujiwara Macromolecular Composition of Stress Fiber–Plasma Membrane Attachment Sites in Endothelial Cells In Situ Circ. Res., November 1, 1996; 79(5): 1000 - 1006. [Abstract] [Full Text]
|
|
|
|
|
|
S. R.P. Gudi, C. B. Clark, and J. A. Frangos Fluid Flow Rapidly Activates G Proteins in Human Endothelial Cells: Involvement of G Proteins in Mechanochemical Signal Transduction Circ. Res., October 1, 1996; 79(4): 834 - 839. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. M. Kirshbom, M. T. Jacobs, S. S. L. Tsui, L. R. DiBernardo, D. A. Schwinn, R. M. Ungerleider, and J. W. Gaynor EFFECTS OF CARDIOPULMONARY BYPASS AND CIRCULATORY ARREST ON ENDOTHELIUM-DEPENDENT VASODILATATION IN THE LUNG J. Thorac. Cardiovasc. Surg., June 1, 1996; 111(6): 1248 - 1256. [Abstract] [Full Text]
|
|
|
|
|
|
P. Palestini, C. Calvi, E. Conforti, L. Botto, C. Fenoglio, and G. Miserocchi Composition, biophysical properties, and morphometry of plasma membranes in pulmonary interstitial edema Am J Physiol Lung Cell Mol Physiol, June 1, 2002; 282(6): L1382 - L1390. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
