Am J Physiol Cell Physiol AJP citation statistics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 267: C554-C562, 1994;
0363-6143/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prevarskaya, N.
Right arrow Articles by Dufy, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prevarskaya, N.
Right arrow Articles by Dufy, B.

AJP - Cell Physiology, Vol 267, Issue 2 C554-C562, Copyright © 1994 by American Physiological Society

ARTICLES

Early effects of PRL on ion conductances in CHO cells expressing PRL receptor

N. Prevarskaya, R. Skryma, P. Vacher, N. Daniel, C. Bignon, J. Djiane and B. Dufy
Laboratory of Neurophysiology, University of Bordeaux II, France.

Chinese hamster ovary (CHO-K1) cells were stably transfected with prolactin (PRL) receptor cDNA. These cells (CHO-E32) expressed the long form of functional PRL receptor. Using microfluorimetric and patch-clamp techniques, we have investigated the effects of PRL on intracellular Ca2+ concentration ([Ca2+]i) and membrane ion conductances. Exposure of CHO-E32 cells to 5 nM PRL resulted in an increase in [Ca2+]i. Two types of response were observed: 1) a stimulation of Ca2+ entry and 2) an intracellular Ca2+ mobilization. As PRL inhibited voltage-activated Ca2+ current, the PRL-induced Ca2+ increase does not involve voltage-activated Ca2+ channels. PRL also increased a charybdotoxin-sensitive Ca(2+)-dependent K+ conductance. Simultaneous measurements showed that PRL hyperpolarized the membrane potential before increasing intracellular Ca2+ levels. In voltage clamp, hyperpolarizing voltage steps were associated with increased Ca2+ concentrations, whereas depolarizing voltage steps decreased [Ca2+]i. Cell-free patch-clamp experiments showed that PRL directly stimulates K+ channel activity. Our results suggest the existence of a regulatory complex involving a protein kinase tightly associated with the Ca(2+)-activated K+ channels and that PRL stimulates these channels by means of the activation of protein kinase. The resulting hyperpolarization stimulates Ca2+ entry, probably through voltage-insensitive nonspecific channels.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
I. C. Kokay, P. M. Bull, R. L. Davis, M. Ludwig, and D. R. Grattan
Expression of the long form of the prolactin receptor in magnocellular oxytocin neurons is associated with specific prolactin regulation of oxytocin neurons
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1216 - R1225.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. M. Shuba, N. Prevarskaya, L. Lemonnier, F. Van Coppenolle, P. G. Kostyuk, B. Mauroy, and R. Skryma
Volume-regulated chloride conductance in the LNCaP human prostate cancer cell line
Am J Physiol Cell Physiol, October 1, 2000; 279(4): C1144 - C1154.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Sorin, O. Goupille, A. M. Vacher, J. Paly, J. Djiane, and P. Vacher
Distinct Cytoplasmic Regions of the Prolactin Receptor Are Required for Prolactin-induced Calcium Entry
J. Biol. Chem., October 23, 1998; 273(43): 28461 - 28469.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online