Am J Physiol Cell Physiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 267: C32-C38, 1994;
0363-6143/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coleman, R. A.
Right arrow Articles by Wade, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coleman, R. A.
Right arrow Articles by Wade, J. B.

AJP - Cell Physiology, Vol 267, Issue 1 C32-C38, Copyright © 1994 by American Physiological Society

ARTICLES

ADH-induced recycling of fluid-phase marker from endosomes to the mucosal surface in toad bladder

R. A. Coleman and J. B. Wade
Department of Physiology, University of Maryland School of Medicine, Baltimore 21201.

In the toad urinary bladder, the reversal of antidiuretic hormone (ADH) stimulation results in the endocytosis of apical membrane water channels, along with any fluid-phase marker present in the mucosal bathing solution. We have loaded vesicles with horseradish peroxidase (HRP), then restimulated the bladders and measured the reappearance of endocytosed HRP in the mucosal bath. HRP-loaded bladders that were restimulated showed HRP release that peaked sharply within 15 min after restimulation. Varying the interval between loading and restimulation did not vary HRP release significantly. Restimulation with forskolin gave HRP release values similar to ADH. The amount of HRP released correlated with the magnitude of water permeability induced. The demonstration that fluid-phase markers can be recycled from endosomes to the apical surface in a hormone-dependent fashion indicates that endocytosed membrane, containing water channels, is able to rapidly recycle back to the surface in response to hormone restimulation. In addition, marker release declined progressively with repeated restimulation, totaling < 30% of the retrieved amount. This result indicates that a relatively large proportion of the retrieved marker reaches a nonrecycling compartment.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
M. B. Butterworth, R. S. Edinger, R. A. Frizzell, and J. P. Johnson
Regulation of the epithelial sodium channel by membrane trafficking
Am J Physiol Renal Physiol, January 1, 2009; 296(1): F10 - F24.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Denton, D. Boahene, and W. M. Moran
Luminal L-alanine stimulates exocytosis at the K+-conductive apical membrane of Aplysia enterocytes
Am J Physiol Cell Physiol, November 1, 1998; 275(5): C1300 - C1312.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online