| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 266, Issue 6 C1795-C1802, Copyright © 1994 by American Physiological Society
ARTICLES |
D. K. McMahon, P. A. Anderson, R. Nassar, J. B. Bunting, Z. Saba, A. E. Oakeley and N. N. Malouf
Department of Pathology, University of North Carolina at Chapel Hill 27599.
We examined the myofibril biochemical, structural, and biophysical properties of C2C12, a mouse skeletal muscle cell line (American Type Culture Collection), to assess whether force development and the sensitivity of the myofilaments to calcium could be measured in C2C12 myotubes and whether a cardiac contractile protein, troponin T, is expressed and incorporated into C2C12 myofibrils. When myoblasts fused and differentiated into myotubes, expression of myofilament proteins was initiated. Multiple cardiac and skeletal muscle troponin T isoforms were coexpressed. Cardiac troponin T expression increased and then decreased with time. Fluorescence immunocytochemistry demonstrated incorporation of cardiac troponin T isoforms into the myofibrils. At the time of the biophysical studies, mean myotube diameter was 12 microns (range 5-25 microns), and mean length was 290 microns (range 130-520 microns). The estimated maximum force developed by chemically skinned myotubes at 6-7 days poststarvation, 0.88 +/- 0.12 microN (mean +/- 95% confidence interval, n = 5), was significantly less (P < 0.05) than that at 10-13 days poststarvation, 1.12 +/- 0.12 microN (n = 7). The force-pCa relation yielded a Hill coefficient of 2.9 +/- 0.6 (n = 7) and half-maximal activation at pCa of 5.77 +/- 0.20. The demonstration that the biophysical properties of C2C12 cells can be measured and that cardiac and skeletal muscle troponin T isoforms are incorporated and colocalized into myofibrils suggest that these cells could be a useful model to assess the effects of exogenous native and mutated cardiac and skeletal contractile protein isoforms on myofilament function.
This article has been cited by other articles:
|
|
 |
|
  B. Fioretti, T. Pietrangelo, L. Catacuzzeno, and F. Franciolini Intermediate-conductance Ca2+-activated K+ channel is expressed in C2C12 myoblasts and is downregulated during myogenesis Am J Physiol Cell Physiol, July 1, 2005; 289(1): C89 - C96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Nassar, N. N. Malouf, L. Mao, H. A. Rockman, A. E. Oakeley, J. R. Frye, J. R. Herlong, S. P. Sanders, and P. A. W. Anderson cTnT1, a cardiac troponin T isoform, decreases myofilament tension and affects the left ventricular pressure waveform Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1147 - H1156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fredericks, J. F. Murray, N. D. Carter, A. M.S. Chesser, S. Papachristou, M. M. Yaqoob, P. O. Collinson, D. Gaze, and D. W. Holt Cardiac Troponin T and Creatine Kinase MB Content in Skeletal Muscle of the Uremic Rat Clin. Chem., June 1, 2002; 48(6): 859 - 868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cabrero, M. Alegret, R. M. Sanchez, T. Adzet, J. C. Laguna, and M. V. Carrera Increased Reactive Oxygen Species Production Down-regulates Peroxisome Proliferator-activated alpha Pathway in C2C12 Skeletal Muscle Cells J. Biol. Chem., March 15, 2002; 277(12): 10100 - 10107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. A. Heunks, H. A. Machiels, P. N. R. Dekhuijzen, Y. S. Prakash, and G. C. Sieck Nitric oxide affects sarcoplasmic calcium release in skeletal myotubes J Appl Physiol, November 1, 2001; 91(5): 2117 - 2124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fredericks, J. F. Murray, M. Bewick, R. Chang, P. O. Collinson, N. D. Carter, and D. W. Holt Cardiac Troponin T and Creatine Kinase MB Are Not Increased in Exterior Oblique Muscle of Patients with Renal Failure Clin. Chem., June 1, 2001; 47(6): 1023 - 1030. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. N. Malouf, W. B. Coleman, J. W. Grisham, R. A. Lininger, V. J. Madden, M. Sproul, and P. A. W. Anderson Adult-Derived Stem Cells from the Liver Become Myocytes in the Heart in Vivo Am. J. Pathol., June 1, 2001; 158(6): 1929 - 1935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Liu and E. Engvall Sarcoglycan Isoforms in Skeletal Muscle J. Biol. Chem., December 31, 1999; 274(53): 38171 - 38176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ricchiuti and F. S. Apple RNA Expression of Cardiac Troponin T Isoforms in Diseased Human Skeletal Muscle Clin. Chem., December 1, 1999; 45(12): 2129 - 2135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ricchiuti, E. M. Voss, A. Ney, M. Odland, P. A. W. Anderson, and F. S. Apple Cardiac troponin T isoforms expressed in renal diseased skeletal muscle will not cause false-positive results by the second generation cardiac troponin T assay by Boehringer Mannheim Clin. Chem., September 1, 1998; 44(9): 1919 - 1924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rezvani and C.-C. Liew Role of the Adenomatous Polyposis Coli Gene Product in Human Cardiac Development and Disease J. Biol. Chem., June 9, 2000; 275(24): 18470 - 18475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Formigli, F. Francini, E. Meacci, M. Vassalli, D. Nosi, F. Quercioli, B. Tiribilli, C. Bencini, C. Piperio, P. Bruni, et al. Sphingosine 1-phosphate induces Ca2+ transients and cytoskeletal rearrangement in C2C12 myoblastic cells Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1361 - C1373. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
