Am J Physiol Cell Physiol AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 266: C1775-C1782, 1994;
0363-6143/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (58)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Escalante, B.
Right arrow Articles by McGiff, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Escalante, B.
Right arrow Articles by McGiff, J. C.

AJP - Cell Physiology, Vol 266, Issue 6 C1775-C1782, Copyright © 1994 by American Physiological Society

ARTICLES

Cytochrome P-450 arachidonate metabolites affect ion fluxes in rabbit medullary thick ascending limb

B. Escalante, D. Erlij, J. R. Falck and J. C. McGiff
Department of Pharmacology, New York Medical College, Valhalla 10595.

The medullary thick ascending limb of Henle's loop (mTALH) of the rabbit metabolizes arachidonic acid (AA) via a cytochrome P-450 (P-450) monooxygenase pathway to several products, of which the principal are 20-hydroxy-5,8,11,14-eicosatetraenoic acid (20-HETE) and 1,20-eicosatetraenedioic acid (20-COOH-AA). To understand their mechanism of action on alkali cation metabolism in mTALH cells, we have compared their effects with those of ouabain and furosemide. Incubation of rabbit isolated mTALH cells with either 1 mM ouabain or furosemide decreased K+ content from a control of 1,015 +/- 51 peq/micrograms protein to 717 +/- 41 and to 548 +/- 48 peq/micrograms protein, respectively, whereas they had opposite effects on Na+ content; from a control of 138 +/- 22 peq/micrograms protein, ouabain increased Na+ content to 357 +/- 37 peq/micrograms protein, and furosemide decreased it to 64 +/- 23 peq/micrograms protein. Preincubation with either 20-HETE (1 microM) or 20-COOH-AA (1 microM) decreased Na+ and K+, resembling furosemide in their effects on Na+ and K+ content. In other experiments we used monensin-treated cells to determine 86Rb uptake under conditions in which Na+ entry into the cell was not rate limiting. Under these conditions ouabain still inhibited 86Rb uptake, and the effect of AA was blocked. A major action of AA metabolites on Na(+)-K(+)-adenosinetriphosphatase was thereby excluded. Furthermore, AA metabolites did not inhibit Ba(2+)-sensitive 86Rb efflux, indicating that they do not act through K+ channels.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Inoue, K. Sodhi, N. Puri, K. H. Gotlinger, J. Cao, R. Rezzani, J. R. Falck, N. G. Abraham, and M. Laniado-Schwartzman
Endothelial-specific CYP4A2 overexpression leads to renal injury and hypertension via increased production of 20-HETE
Am J Physiol Renal Physiol, October 1, 2009; 297(4): F875 - F884.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Yu, B. Lopez, E. A. Dos Santos, J. R. Falck, and R. J. Roman
Effects of 20-HETE on Na+ transport and Na+-K+-ATPase activity in the thick ascending loop of Henle
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2007; 292(6): R2400 - R2405.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Huang, Y. Zhou, V. T. Raju, J. Du, H.-H. Chang, C.-Y. Wang, M. W. Brands, J. R. Falck, and M.-H. Wang
Renal 20-HETE inhibition attenuates changes in renal hemodynamics induced by L-NAME treatment in pregnant rats
Am J Physiol Renal Physiol, November 1, 2005; 289(5): F1116 - F1122.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
K. M. Hoagland, A. K. Flasch, and R. J. Roman
Inhibitors of 20-HETE Formation Promote Salt-Sensitive Hypertension in Rats
Hypertension, October 1, 2003; 42(4): 669 - 673.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. Zhu, C. Zhang, M. Medhora, and E. R. Jacobs
CYP4A mRNA, protein, and product in rat lungs: novel localization in vascular endothelium
J Appl Physiol, July 1, 2002; 93(1): 330 - 337.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
R. J. Roman
P-450 Metabolites of Arachidonic Acid in the Control of Cardiovascular Function
Physiol Rev, January 1, 2002; 82(1): 131 - 185.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M.-H. Wang, F. Zhang, J. Marji, B. A. Zand, A. Nasjletti, and M. Laniado-Schwartzman
CYP4A1 antisense oligonucleotide reduces mesenteric vascular reactivity and blood pressure in SHR
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2001; 280(1): R255 - R261.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Quigley, M. Baum, K. M. Reddy, J. C. Griener, and J. R. Falck
Effects of 20-HETE and 19(S)-HETE on rabbit proximal straight tubule volume transport
Am J Physiol Renal Physiol, June 1, 2000; 278(6): F949 - F953.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
J. H. Capdevila, J. R. Falck, and R. C. Harris
Cytochrome P450 and arachidonic acid bioactivation: molecular and functional properties of the arachidonate monooxygenase
J. Lipid Res., February 1, 2000; 41(2): 163 - 181.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. C. McGiff and J. Quilley
20-HETE and the kidney: resolution of old problems and new beginnings
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 1999; 277(3): R607 - R623.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. W. Good, T. George, and D. H. Wang
Angiotensin II inhibits HCO-3 absorption via a cytochrome P-450-dependent pathway in MTAL
Am J Physiol Renal Physiol, May 1, 1999; 276(5): F726 - F736.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
O. Ito and R. J. Roman
Role of 20-HETE in Elevating Chloride Transport in the Thick Ascending Limb of Dahl SS/Jr Rats
Hypertension, January 1, 1999; 33(1): 419 - 423.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Amlal, C. LeGoff, C. Vernimmen, M. Soleimani, M. Paillard, and M. Bichara
ANG II controls Na+-K+(NH+4)-2Cl- cotransport via 20-HETE and PKC in medullary thick ascending limb
Am J Physiol Cell Physiol, April 1, 1998; 274(4): C1047 - C1056.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M.-H. Wang, E. Brand-Schieber, B. A. Zand, X. Nguyen, J. R. Falck, N. Balu, and M. L. Schwartzman
Cytochrome P450-Derived Arachidonic Acid Metabolism in the Rat Kidney: Characterization of Selective Inhibitors
J. Pharmacol. Exp. Ther., March 1, 1998; 284(3): 966 - 973.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. Schnermann
Juxtaglomerular cell complex in the regulation of renal salt excretion
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1998; 274(2): R263 - R279.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
N. R. Ferreri, B. A. Escalante, Y. Zhao, S.-J. An, and J. C. McGiff
Angiotensin II induces TNF production by the thick ascending limb: functional implications
Am J Physiol Renal Physiol, January 1, 1998; 274(1): F148 - F155.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. A. Escalante, J. C. McGiff, and A. O. Oyekan
Role of cytochrome P-450 arachidonate metabolites in endothelin signaling in rat proximal tubule
Am J Physiol Renal Physiol, January 1, 2002; 282(1): F144 - F150.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online