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Am J Physiol Cell Physiol 266: C1692-C1698, 1994;
0363-6143/94 $5.00
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AJP - Cell Physiology, Vol 266, Issue 6 C1692-C1698, Copyright © 1994 by American Physiological Society

ARTICLES

ATP decreases acutely and reversibly transport through the paracellular pathway in human cervical cells

G. I. Gorodeski, U. Hopfer, R. L. Eckert, W. H. Utian, B. J. De Santis, E. A. Rorke and M. F. Romero
Department of Reproductive Biology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.

We studied the effect of ATP on transepithelial transport through the paracellular pathway in human cervical cells. Transepithelial conductance and transepithelial permeability (determined from the measurements of unidirectional flux of inert molecules) were measured in Caski cells grown on permeable support. Transepithelial conductance was 55.9 +/- 17.7 mS/cm2 and permeability was 12.5 +/- 2.7 x 10(-6) cm/s for a 0.51-kDa probe. Addition of ATP to the medium decreased acutely and reversibly the conductance and the permeability to probes between 0.18 and 10 kDa by 23-31% in a dose-related fashion; the 50% effective concentration was 1 microM, with a maximal effect at 5-10 microM extracellular ATP. The ATP effect was observed regardless of the pressure gradient across the epithelium. These results indicate that extracellular ATP in micromolar concentrations decreases acutely and reversibly the permeability through the paracellular pathway in cervical cells, possibly by affecting the permeability of the tight junctions and the resistance of the intercellular space. On the basis of these data, we speculate that ATP may play a role in the regulation of solutes and fluid transport across the cervical epithelium in vivo.


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G. I. Gorodeski, R. L. Eckert, D. Pal, W. H. Utian, and E. A. Rorke
Retinoids regulate tight junctional resistance of cultured human cervical cells
Am J Physiol Cell Physiol, November 1, 1997; 273(5): C1707 - C1713.
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