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Am J Physiol Cell Physiol 266: C1538-C1543, 1994;
0363-6143/94 $5.00
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AJP - Cell Physiology, Vol 266, Issue 6 C1538-C1543, Copyright © 1994 by American Physiological Society

ARTICLES

A Na(+)-sensitive cation channel modulated by angiotensin II in cultured intestinal myocytes

V. L. Nouailhetas, J. Aboulafia, E. Frediani-Neto, A. T. Ferreira and A. C. Paiva
Department of Biophysics, Escola Paulista de Medicina, Sao Paulo, Brazil.

Single-channel currents were recorded in excised inside-out and cell-attached patches of cultured cells from the longitudinal smooth muscle of the guinea pig ileum. In the presence of symmetrical high-K+ solutions, we identified a voltage-dependent 12-pS channel. It was reversibly blocked by addition of either Ba2+ or Cs+ at the cellular side of the patch but was insensitive to Ca2+ or ATP. This channel had poor selectivity concerning cations (PLi > PK = PNa = PCa, where P is permeability) and low permeability to anions. Isosmotic substitution of NaCl for KCl in the solution facing the cellular side enhanced the channel activity by increasing NPo values where N is number of channels and Po is open probability. In the cell-attached configuration, the channel was also activated by addition of angiotensin II in the bath solution. We propose that this nonselective cation channel might play a role in the control of the membrane potential during the contractile response of the guinea pig ileum to agonists by keeping the voltage-sensitive Ca2+ channels open.


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