Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 266: C809-C824, 1994;
0363-6143/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhou, H.
Right arrow Articles by Palmer, L. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhou, H.
Right arrow Articles by Palmer, L. G.

AJP - Cell Physiology, Vol 266, Issue 3 C809-C824, Copyright © 1994 by American Physiological Society

ARTICLES

Primary structure and functional properties of an epithelial K channel

H. Zhou, S. S. Tate and L. G. Palmer
Department of Physiology and Biophysics, Cornell University Medical College, New York, New York 10021.

Expression cloning in Xenopus oocytes was used to identify a clone for a renal K channel. The clone, named ROMK2, was obtained from a cDNA library constructed in the plasmid vector pSPORT using size-selected poly(A)+ RNA from whole rat kidney. ROMK2 consists of 1,837 nucleotides, with an open reading frame of 1,116 bases predicted to code for a 372-amino acid peptide. The clone appears to be a splice variant of a recently reported K channel (ROMK1) from rat renal outer medulla (Ho, K.H., C.G. Nichols, W.J. Lederer, J. Lytton, P.M. Vassilev, M.V. Kanazirska, and S.C. Hebert. Nature Lond. 362: 31-37, 1993). Northern blot analysis indicates that ROMK2 is expressed in renal cortex, medulla, and papilla. Expression in other tissues appears to be much lower. The functional properties of the channel as measured in Xenopus oocytes indicate its close relationship to ROMK1 and more distant relationship to the inward rectifier K channel (IRK1) (Kubo, Y, T.J. Baldwin, Y. N. Jan, and L. Y. Jan. Nature Lond. 362: 127-133, 1993). The inward conductance of the channel is a saturable function of external K, with a half-maximal conductance at <5 mM. The selectivity sequence for ion permeability based on reversal potential measurements was K > Rb > NH4 > Na, Li. The conductance to Rb was only one-half that to K. Extracellular Ba2+ and Cs+ blocked the channel in a voltage-dependent manner. The high sensitivity of Cs+ block to voltage is consistent with the channel's operating as a multi-ion pore. The channel was blocked by high concentrations (100 microM) of glibenclamide. It did not appear to be blocked by extracellular Na+ or tetraethyl-ammonium ion. Patch-clamp measurements indicated a single-channel conductance of 30 pS in the presence of 110 mM K and high open probability that was weakly dependent on voltage. This channel may be involved in maintaining the membrane potential of renal cells and/or mediating renal K secretion.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
L. M. Satlin, M. D. Carattino, W. Liu, and T. R. Kleyman
Regulation of cation transport in the distal nephron by mechanical forces
Am J Physiol Renal Physiol, November 1, 2006; 291(5): F923 - F931.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. C. Hebert, G. Desir, G. Giebisch, and W. Wang
Molecular Diversity and Regulation of Renal Potassium Channels
Physiol Rev, January 1, 2005; 85(1): 319 - 371.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
G. Frindt and L. G. Palmer
Apical potassium channels in the rat connecting tubule
Am J Physiol Renal Physiol, November 1, 2004; 287(5): F1030 - F1037.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P.-Y. Chu, R. Quigley, V. Babich, and C.-L. Huang
Dietary potassium restriction stimulates endocytosis of ROMK channel in rat cortical collecting duct
Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1179 - F1187.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
W.-Z. Zeng, V. Babich, B. Ortega, R. Quigley, S. J. White, P. A. Welling, and C.-L. Huang
Evidence for endocytosis of ROMK potassium channel via clathrin-coated vesicles
Am J Physiol Renal Physiol, October 1, 2002; 283(4): F630 - F639.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A.-A. Konstas, M. Dabrowski, C. Korbmacher, and S. J. Tucker
Intrinsic Sensitivity of Kir1.1 (ROMK) to Glibenclamide in the Absence of SUR2B. IMPLICATIONS FOR THE IDENTITY OF THE RENAL ATP-REGULATED SECRETORY K+ CHANNEL
J. Biol. Chem., June 7, 2002; 277(24): 21346 - 21351.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Dong, J. Xu, C. G. Vanoye, R. Welch, G. G. MacGregor, G. Giebisch, and S. C. Hebert
An Amino Acid Triplet in the NH2 Terminus of Rat ROMK1 Determines Interaction with SUR2B
J. Biol. Chem., November 16, 2001; 276(47): 44347 - 44353.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. ADACHI, K. KITAMURA, T. MIYOSHI, T. NARIKIYO, K. IWASHITA, N. SHIRAISHI, H. NONOGUCHI, and K. TOMITA
Activation of Epithelial Sodium Channels by Prostasin in Xenopus Oocytes
J. Am. Soc. Nephrol., June 1, 2001; 12(6): 1114 - 1121.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. M. Satlin, S. Sheng, C. B. Woda, and T. R. Kleyman
Epithelial Na+ channels are regulated by flow
Am J Physiol Renal Physiol, June 1, 2001; 280(6): F1010 - F1018.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. B. Woda, A. Bragin, T. R. Kleyman, and L. M. Satlin
Flow-dependent K+ secretion in the cortical collecting duct is mediated by a maxi-K channel
Am J Physiol Renal Physiol, May 1, 2001; 280(5): F786 - F793.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P.-P. Duquette, P. Bissonnette, and J.-Y. Lapointe
Local osmotic gradients drive the water flux associated with Na+/glucose cotransport
PNAS, March 27, 2001; 98(7): 3796 - 3801.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. Muto
Potassium Transport in the Mammalian Collecting Duct
Physiol Rev, January 1, 2001; 81(1): 85 - 116.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
C. A. ECELBARGER, G.-H. KIM, M. A. KNEPPER, J. LIU, M. TATE, P. A. WELLING, and J. B. WADE
Regulation of Potassium Channel Kir 1.1 (ROMK) Abundance in the Thick Ascending Limb of Henle's Loop
J. Am. Soc. Nephrol., January 1, 2001; 12(1): 10 - 18.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Leipziger, G. G. MacGregor, G. J. Cooper, J. Xu, S. C. Hebert, and G. Giebisch
PKA site mutations of ROMK2 channels shift the pH dependence to more alkaline values
Am J Physiol Renal Physiol, November 1, 2000; 279(5): F919 - F926.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. A. Mennitt, G. Frindt, R. B. Silver, and L. G. Palmer
Potassium restriction downregulates ROMK expression in rat kidney
Am J Physiol Renal Physiol, June 1, 2000; 278(6): F916 - F924.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-M. Leung, W.-Z. Zeng, H.-H. Liou, C. R. Solaro, and C.-L. Huang
Phosphatidylinositol 4,5-Bisphosphate and Intracellular pH Regulate the ROMK1 Potassium Channel via Separate but Interrelated Mechanisms
J. Biol. Chem., March 31, 2000; 275(14): 10182 - 10189.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Chanchevalap, Z. Yang, N. Cui, Z. Qu, G. Zhu, C. Liu, L. R. Giwa, L. Abdulkadir, and C. Jiang
Involvement of Histidine Residues in Proton Sensing of ROMK1 Channel
J. Biol. Chem., March 10, 2000; 275(11): 7811 - 7817.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. G. Palmer
Potassium secretion and the regulation of distal nephron K channels
Am J Physiol Renal Physiol, December 1, 1999; 277(6): F821 - F825.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Zolotnitskaya and L. M. Satlin
Developmental expression of ROMK in rat kidney
Am J Physiol Renal Physiol, June 1, 1999; 276(6): F825 - F836.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Chang and T. Fujita
A numerical model of the renal distal tubule
Am J Physiol Renal Physiol, June 1, 1999; 276(6): F931 - F951.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. H. Beesley, B. Ortega, and S. J. White
Splicing of a retained intron within ROMK K+ channel RNA generates a novel set of isoforms in rat kidney
Am J Physiol Cell Physiol, March 1, 1999; 276(3): C585 - C592.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. M. SCHWIEBERT, D. J. BENOS, M. E. EGAN, M. J. STUTTS, and W. B. GUGGINO
CFTR Is a Conductance Regulator as well as a Chloride Channel
Physiol Rev, January 1, 1999; 79(1): 145 - 166.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
M. Yamada, A. Inanobe, and Y. Kurachi
G Protein Regulation of Potassium Ion Channels
Pharmacol. Rev., December 1, 1998; 50(4): 723 - 757.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. M. McNicholas, G. G. MacGregor, L. D. Islas, Y. Yang, S. C. Hebert, and G. Giebisch
pH-dependent modulation of the cloned renal K+ channel, ROMK
Am J Physiol Renal Physiol, December 1, 1998; 275(6): F972 - F981.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. C. Hebert
Roles of Na-K-2Cl and Na-Cl cotransporters and ROMK potassium channels in urinary concentrating mechanism
Am J Physiol Renal Physiol, September 1, 1998; 275(3): F325 - F327.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
G. G. MacGregor, J. Z. Xu, C. M. McNicholas, G. Giebisch, and S. C. Hebert
Partially active channels produced by PKA site mutation of the cloned renal K+ channel, ROMK2 (kir1.2)
Am J Physiol Renal Physiol, September 1, 1998; 275(3): F415 - F422.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Wald, H. Garty, L. G. Palmer, and M. M. Popovtzer
Differential regulation of ROMK expression in kidney cortex and medulla by aldosterone and potassium
Am J Physiol Renal Physiol, August 1, 1998; 275(2): F239 - F245.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
G. Giebisch
Renal potassium transport: mechanisms and regulation
Am J Physiol Renal Physiol, May 1, 1998; 274(5): F817 - F833.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
G. Frindt, H. Zhou, H. Sackin, and L. G. Palmer
Dissociation of K channel density and ROMK mRNA in rat cortical collecting tubule during K adaptation
Am J Physiol Renal Physiol, March 1, 1998; 274(3): F525 - F531.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. A. Welling
Primary structure and functional expression of a cortical collecting duct Kir channel
Am J Physiol Renal Physiol, November 1, 1997; 273(5): F825 - F836.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. M. McNicholas, M. W. Nason Jr., W. B. Guggino, E. M. Schwiebert, S. C. Hebert, G. Giebisch, and M. E. Egan
A functional CFTR-NBF1 is required for ROMK2-CFTR interaction
Am J Physiol Renal Physiol, November 1, 1997; 273(5): F843 - F848.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. A. Schwalbe, L. Bianchi, and A. M. Brown
Mapping the Kidney Potassium Channel ROMK1. GLYCOSYLATION OF THE PORE SIGNATURE SEQUENCE AND THE COOH TERMINUS
J. Biol. Chem., October 3, 1997; 272(40): 25217 - 25223.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Choe, H. Zhou, L. G. Palmer, and H. Sackin
A conserved cytoplasmic region of ROMK modulates pH sensitivity, conductance, and gating
Am J Physiol Renal Physiol, October 1, 1997; 273(4): F516 - F529.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Karschin, E. Dissmann, W. Stuhmer, and A. Karschin
IRK(1-3) and GIRK(1-4) Inwardly Rectifying K+ Channel mRNAs Are Differentially Expressed in the Adult Rat Brain
J. Neurosci., June 1, 1996; 16(11): 3559 - 3570.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
W.-Z. Zeng, H.-H. Liou, U. M. Krishna, J. R. Falck, and C.-L. Huang
Structural determinants and specificities for ROMK1-phosphoinositide interaction
Am J Physiol Renal Physiol, May 1, 2002; 282(5): F826 - F834.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online