AJP - Cell Physiology, Vol 266, Issue 1 C189-C197, Copyright © 1994 by American Physiological Society

ARTICLES

Expression, functional analysis, and in situ hybridization of a cloned rat kidney collecting duct water channel

T. Ma, H. Hasegawa, W. R. Skach, A. Frigeri and A. S. Verkman
Department of Medicine, University of California, San Francisco 94143.

The cloning and expression of an apical membrane water channel from rat kidney collecting duct (WCH-CD) homologous to a 28-kDa integral membrane protein (CHIP28) was reported recently (K. Fushimi, S. Uchida, Y. Hara, Y. Hirata, F. Marumo, and S. Sasaki. Nature Lond. 361: 549-552, 1993). We obtained an approximately 1.8-kilobase clone from a rat kidney lambda gt10 cDNA library by a polymerase chain reaction cloning method; whereas the coding sequence (814 base pairs, predicted protein size 29 kDa) was identical to that reported, we identified an in-frame ATG codon at base pair -123 predicting a protein size of 33 kDa. On Northern blots probed by cDNAs corresponding to the WCH-CD coding sequence (base pairs +1 to +814) or 5'-untranslated sequence (-403 to -16), a single band at 1.9 kilobases was observed in kidney medulla greater than in cortex but not in other tissues; mRNA expression was increased strongly by dehydration. Translation and oocyte expression studies were performed to identify the translation start site. The short (base pairs +1 to +814) and long (base pairs -123 to +814) cDNAs were subcloned in vector pSP64 containing the 5'-untranslated Xenopus globin sequence upstream to the ATGs; a 30-base pair c-myc sequence was engineered at the COOH- terminal for antibody recognition.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				Y.-J. Lee, I.-K. Song, K.-J. Jang, J. Nielsen, J. Frokiaer, S. Nielsen, and T.-H. Kwon Increased AQP2 targeting in primary cultured IMCD cells in response to angiotensin II through AT1 receptor Am J Physiol Renal Physiol, January 1, 2007; 292(1): F340 - F350. [Abstract] [Full Text] [PDF]

				U. Hasler, S. Nielsen, E. Feraille, and P.-Y. Martin Posttranscriptional control of aquaporin-2 abundance by vasopressin in renal collecting duct principal cells Am J Physiol Renal Physiol, January 1, 2006; 290(1): F177 - F187. [Abstract] [Full Text] [PDF]

				L. N. Nejsum, M. Zelenina, A. Aperia, J. Frokiaer, and S. Nielsen Bidirectional regulation of AQP2 trafficking and recycling: involvement of AQP2-S256 phosphorylation Am J Physiol Renal Physiol, May 1, 2005; 288(5): F930 - F938. [Abstract] [Full Text] [PDF]

				L. Brond, N. Hadrup, N. Salling, M. Torp, M. Graebe, S. Christensen, S. Nielsen, and T. E. N. Jonassen Uncoupling of vasopressin signaling in collecting ducts from rats with CBL-induced liver cirrhosis Am J Physiol Renal Physiol, October 1, 2004; 287(4): F806 - F815. [Abstract] [Full Text] [PDF]

				S. Nielsen, J. Frokiar, D. Marples, T.-H. Kwon, P. Agre, and M. A. Knepper Aquaporins in the Kidney: From Molecules to Medicine Physiol Rev, January 1, 2002; 82(1): 205 - 244. [Abstract] [Full Text] [PDF]

				T. E. N. Jonassen, S. Christensen, T.-H. Kwon, S. Langhoff, N. Salling, and S. Nielsen Renal water handling in rats with decompensated liver cirrhosis Am J Physiol Renal Physiol, December 1, 2000; 279(6): F1101 - F1109. [Abstract] [Full Text] [PDF]

				C. Shayakul, C. P. Smith, H. S. Mackenzie, W.-S. Lee, D. Brown, and M. A. Hediger Long-term regulation of urea transporter expression by vasopressin in Brattleboro rats Am J Physiol Renal Physiol, April 1, 2000; 278(4): F620 - F627. [Abstract] [Full Text] [PDF]

				T. E. N. Jonassen, D. Promeneur, S. Christensen, J. S. Petersen, and S. Nielsen Decreased vasopressin-mediated renal water reabsorption in rats with chronic aldosterone-receptor blockade Am J Physiol Renal Physiol, February 1, 2000; 278(2): F246 - F256. [Abstract] [Full Text] [PDF]

				T. Zeuthen and D. A. Klaerke Transport of Water and Glycerol in Aquaporin 3 Is Gated by H+ J. Biol. Chem., July 30, 1999; 274(31): 21631 - 21636. [Abstract] [Full Text] [PDF]

				T. E. N. Jonassen, S. Nielsen, S. Christensen, and J. S. Petersen Decreased vasopressin-mediated renal water reabsorption in rats with compensated liver cirrhosis Am J Physiol Renal Physiol, August 1, 1998; 275(2): F216 - F225. [Abstract] [Full Text] [PDF]

				B. M. Christensen, D. Marples, U. B. Jensen, J. Frokiaer, D. Sheikh-Hamad, M. Knepper, and S. Nielsen Acute effects of vasopressin V2-receptor antagonist on kidney AQP2 expression and subcellular distribution Am J Physiol Renal Physiol, August 1, 1998; 275(2): F285 - F297. [Abstract] [Full Text] [PDF]

				T. Ma, B. Yang, M. A. Matthay, and A. S. Verkman Evidence against a Role of Mouse, Rat, and Two Cloned Human T1alpha Isoforms as a Water Channel or a Regulator of Aquaporin-type Water Channels Am. J. Respir. Cell Mol. Biol., July 1, 1998; 19(1): 143 - 149. [Abstract] [Full Text]

				Z. Han, M. B. Wax, and R. V. Patil Regulation of Aquaporin-4 Water Channels by Phorbol Ester-dependent Protein Phosphorylation J. Biol. Chem., March 13, 1998; 273(11): 6001 - 6004. [Abstract] [Full Text] [PDF]

				B. Yang and A. S. Verkman Water and Glycerol Permeabilities of Aquaporins 1-5 and MIP Determined Quantitatively by Expression of Epitope-tagged Constructs in Xenopus Oocytes J. Biol. Chem., June 27, 1997; 272(26): 16140 - 16146. [Abstract] [Full Text] [PDF]