Am J Physiol Cell Physiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 265: C1527-C1543, 1993;
0363-6143/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brundage, R. A.
Right arrow Articles by Fay, F. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brundage, R. A.
Right arrow Articles by Fay, F. S.

AJP - Cell Physiology, Vol 265, Issue 6 C1527-C1543, Copyright © 1993 by American Physiological Society

ARTICLES

Chemotaxis of newt eosinophils: calcium regulation of chemotactic response

R. A. Brundage, K. E. Fogarty, R. A. Tuft and F. S. Fay
Department of Physiology, University of Massachusetts Medical School, Worcester 01605.

Local chemical events underlying chemotaxis were characterized in a new model cell, the newt eosinophil. These cells exhibit a chemotactic response to a trypsin-sensitive component of newt serum. Ca2+ plays a role in this process, since treatments expected to diminish Ca2+ availability from the medium [ethylene glycol-bis (beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid, Co2+, and verapamil], to break down transmembrane Ca2+ gradients (ionomycin), or to interfere with the function of intracellular Ca2+ stores (caffeine and neomycin) inhibited cell polarization and movement. Using imaging techniques we found that cytosolic Ca2+ concentration ([Ca2+]i) increased in response to newt serum. Migrating newt eosinophils exhibited a dynamic heterogeneous distribution of [Ca2+]i. [Ca2+]i was elevated in cells undergoing a change of direction relative to cells migrating persistently in one direction. Migrating cells contained gradients of [Ca2+]i along their long axis, with the front of the cell having consistently lower [Ca2+]i than the rear. When cells were loaded with the cell-permeant form of fura 2, fura 2 acetoxymethyl ester, a caffeine-sensitive membrane-delimited region of elevated [Ca2+]i was seen associated with the microtubule organizing center. A model is proposed relating the distribution of [Ca2+]i and the location of the external stimulus to the generation and interaction of substances within the cell that both simulate and inhibit increases in [Ca2+]i.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
L. Liu, P. Ridefelt, L. Hakansson, and P. Venge
Regulation of Human Eosinophil Migration Across Lung Epithelial Monolayers by Distinct Calcium Signaling Mechanisms in the Two Cell Types
J. Immunol., November 15, 1999; 163(10): 5649 - 5655.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. J. PETTIT
Cytosolic Free Calcium and the Cytoskeleton in the Control of Leukocyte Chemotaxis
Physiol Rev, October 1, 1998; 78(4): 949 - 967.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. E. Hinman, G. J. Beilman, K. E. Groehler, and P. J. Sammak
Wound-induced calcium waves in alveolar type II cells
Am J Physiol Lung Cell Mol Physiol, December 1, 1997; 273(6): L1242 - L1248.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online