| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 265, Issue 6 C1489-C1500, Copyright © 1993 by American Physiological Society
ARTICLES |
P. S. Jackson and K. Strange
Department of Medicine, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
C6 glioma cells accumulate the organic osmolyte inositol in response to chronic hypertonic stress. Upon return to isotonic conditions, cell swelling activates a Na(+)-independent passive low-affinity inositol efflux mechanism that is inhibited 80-100% by a number of anion transport blockers, certain lipoxygenase blockers, and various polyunsaturated fatty acids. Taurine efflux is also enhanced by cell swelling. The taurine efflux pathway has characteristics that are identical to those of the inositol efflux mechanism, including kinetics of activation and inactivation, osmotic sensitivity, pharmacological sensitivity, and inhibition by certain Na+ and Cl- substitutes. These results suggest strongly that volume-sensitive inositol and taurine efflux are mediated by a common transport mechanism. The inhibition of the transport pathway by anion transport blockers and unsaturated fatty acids suggests indirectly that efflux of these solutes may be mediated by an anion channel. Whole cell patch clamp measurements in CsCl solutions were used to test this hypothesis. Under hypertonic conditions, C6 cells had an extremely low membrane conductance (approximately 0.02 nS/pF). After cell swelling, however, whole cell anion conductance was activated rapidly to values up to 1.5-2 nS/pF. This conductance was outwardly rectified and selective for anions and was inhibited 80-100% by blockers of swelling-activated inositol and taurine efflux. The relative taurine permeability (i.e., Ptaurine/PCl) of the conductance was 0.20. Isosmotic replacement of raffinose in the external medium with inositol or sorbitol induced a transient inward current, suggesting that Cl- and these polyols compete for common binding sites on the channel. We conclude that a volume-sensitive anion channel mediates the efflux of structurally diverse organic osmolytes such as taurine and inositol from the cell.
This article has been cited by other articles:
|
|
 |
|
  N. Ullrich, A. Caplanusi, B. Brone, D. Hermans, E. Lariviere, B. Nilius, W. Van Driessche, and J. Eggermont Stimulation by caveolin-1 of the hypotonicity-induced release of taurine and ATP at basolateral, but not apical, membrane of Caco-2 cells Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1287 - C1296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Mongin and H. K. Kimelberg ATP regulates anion channel-mediated organic osmolyte release from cultured rat astrocytes via multiple Ca2+-sensitive mechanisms Am J Physiol Cell Physiol, January 1, 2005; 288(1): C204 - C213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. B. Tomassen, D. Fekkes, H. R. de Jonge, and B. C. Tilly Osmotic swelling-provoked release of organic osmolytes in human intestinal epithelial cells Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1417 - C1422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Carton, D. Hermans, and J. Eggermont Hypotonicity induces membrane protrusions and actin remodeling via activation of small GTPases Rac and Cdc42 in Rat-1 fibroblasts Am J Physiol Cell Physiol, October 1, 2003; 285(4): C935 - C944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Parkerson and H. Sontheimer Contribution of chloride channels to volume regulation of cortical astrocytes Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1460 - C1467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Garber and M. M. Hoffman Cl- and Glutamate- Competition for a Volume-Regulated Anion Channel Biol. Bull., October 1, 2002; 203(2): 194 - 195. [Full Text] [PDF]
|
|
|
|
|
|
I. Carton, D. Trouet, D. Hermans, H. Barth, K. Aktories, G. Droogmans, N. K. Jorgensen, E. K. Hoffmann, B. Nilius, and J. Eggermont RhoA exerts a permissive effect on volume-regulated anion channels in vascular endothelial cells Am J Physiol Cell Physiol, July 1, 2002; 283(1): C115 - C125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik Molecular Structure and Physiological Function of Chloride Channels Physiol Rev, April 1, 2002; 82(2): 503 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R. Cammarata, G. Schafer, S.-W. Chen, Z. Guo, and R. E. Reeves Osmoregulatory Alterations in Taurine Uptake by Cultured Human and Bovine Lens Epithelial Cells Invest. Ophthalmol. Vis. Sci., February 1, 2002; 43(2): 425 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. Shennan and M. Peaker Transport of Milk Constituents by the Mammary Gland Physiol Rev, July 1, 2000; 80(3): 925 - 951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Yorek, J. A. Dunlap, W. Liu, and W. L. Lowe Jr. Normalization of hyperosmotic-induced inositol uptake by renal and endothelial cells is regulated by NF-kappa B Am J Physiol Cell Physiol, May 1, 2000; 278(5): C1011 - C1018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Stutzin, R. Torres, M. Oporto, P. Pacheco, A. L. Eguiguren, L. P. Cid, and F. V. Sepulveda Separate taurine and chloride efflux pathways activated during regulatory volume decrease Am J Physiol Cell Physiol, September 1, 1999; 277(3): C392 - C402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Basarsky, D. Feighan, and B. A. MacVicar Glutamate Release through Volume-Activated Channels during Spreading Depression J. Neurosci., August 1, 1999; 19(15): 6439 - 6445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Carmeliet Cardiac Ionic Currents and Acute Ischemia: From Channels to Arrhythmias Physiol Rev, July 1, 1999; 79(3): 917 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Mongin, J. M. Reddi, C. Charniga, and H. K. Kimelberg [3H]taurine and D-[3H]aspartate release from astrocyte cultures are differently regulated by tyrosine kinases Am J Physiol Cell Physiol, May 1, 1999; 276(5): C1226 - C1230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. SCHULTZ, A. K. SINGH, D. C. DEVOR, and R. J. BRIDGES Pharmacology of CFTR Chloride Channel Activity Physiol Rev, January 1, 1999; 79(1): 109 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Guizouarn and R. Motais Swelling activation of transport pathways in erythrocytes: effects of Cl-, ionic strength, and volume changes Am J Physiol Cell Physiol, January 1, 1999; 276(1): C210 - C220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, X.-Y. Yang, and D. M. Cohen Hypotonicity activates transcription through ERK-dependent and -independent pathways in renal cells Am J Physiol Cell Physiol, October 1, 1998; 275(4): C1104 - C1112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Rutledge, M. Aschner, and H. K. Kimelberg Pharmacological characterization of swelling-induced D-[3H]aspartate release from primary astrocyte cultures Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1511 - C1520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Emma, S. Breton, R. Morrison, S. Wright, and K. Strange Effect of cell swelling on membrane and cytoplasmic distribution of pICln Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1545 - C1551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Park, M. R. Edwards, and P. J. Schofield Swelling detection for volume regulation in the primitive eukaryote Giardia intestinalis: a common feature of volume detection in present-day eukaryotes FASEB J, May 1, 1998; 12(7): 571 - 579. [Abstract] [Full Text]
|
|
|
|
|
|
A. J. Berger, A. C. Hart, and J. M. Kaplan Galpha s-Induced Neurodegeneration in Caenorhabditis elegans J. Neurosci., April 15, 1998; 18(8): 2871 - 2880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Basavappa, S. F. Pedersen, N. K. Jorgensen, J. C. Ellory, and E. K. Hoffmann Swelling-Induced Arachidonic Acid Release via the 85-kDa cPLA2 in Human Neuroblastoma Cells J Neurophysiol, March 1, 1998; 79(3): 1441 - 1449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. LANG, G. L. BUSCH, M. RITTER, H. VOLKL, S. WALDEGGER, E. GULBINS, and D. HAUSSINGER Functional Significance of Cell Volume Regulatory Mechanisms Physiol Rev, January 1, 1998; 78(1): 247 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. G. Romanenko, P. F. Davies, and I. Levitan Dual effect of fluid shear stress on volume-regulated anion current in bovine aortic endothelial cells Am J Physiol Cell Physiol, April 1, 2002; 282(4): C708 - C718. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
