Am J Physiol Cell Physiol AJP: Cell Physiology
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Am J Physiol Cell Physiol 265: C893-C900, 1993;
0363-6143/93 $5.00
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AJP - Cell Physiology, Vol 265, Issue 4 C893-C900, Copyright © 1993 by American Physiological Society

ARTICLES

Zn(II) transport and distribution in rat spermatids

J. G. Reyes, M. P. Arrate, M. Santander, L. Guzman and D. J. Benos
Instituto de Quimica, Universidad Catolica de Valparaiso, Chile.

Zn(II) is an essential trace element. In spermatozoa, Zn(II) modulates metabolism and chromatin condensation. The mechanisms of uptake and distribution of this ion in sperm cells have not been explored. In rat spermatids, our results indicate that 1) 65Zn(II) binds with fast kinetics to a labile, presumably extracellular, compartment. This binding is temperature insensitive and not modified by metabolic inhibitors. 2) Entry of 65Zn(II) in the absence of externally added proteins occurs through a mediated transport system that allows exchange to reach steady state in approximately 15 min at 34 degrees C. 3) Upon entering the cells, 65Zn(II) binds tightly to cellular organelles. 4) Exchangeable Zn(II) bound to cytoplasmic proteins plus free intracellular Zn(II) appears to be < 20% of total exchangeable Zn(II). 5) The intracellular exchangeable Zn(II) compartment is decreased by metabolic inhibitors, showing a direct or indirect link between energy metabolism and cellular Zn(II) levels. 6) 65Zn(II) efflux from rat spermatids is a process with a rate constant of 0.16 +/- 0.13 min-1 at 34 degrees C. This exit rate of Zn(II) is likely to be affected by Zn(II) release from cytoplasmic binding sites or organelles.


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S. Brauchi, M. C. Rauch, I. E. Alfaro, C. Cea, I. I. Concha, D. J. Benos, and J. G. Reyes
Kinetics, molecular basis, and differentiation of L-lactate transport in spermatogenic cells
Am J Physiol Cell Physiol, March 1, 2005; 288(3): C523 - C534.
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