AJP - Cell Physiology, Vol 265, Issue 3 C757-C762, Copyright © 1993 by American Physiological Society

ARTICLES

Vasopressin depolymerizes apical F-actin in rat inner medullary collecting duct

H. Simon, Y. Gao, N. Franki and R. M. Hays
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.

In amphibian bladder, arginine vasopressin (AVP) depolymerizes F-actin in the apical region of the granular cell, promoting fusion of water channel-carrying vesicles with the apical membrane. We now report the effect of AVP on F-actin in the mid- and terminal segments of rat inner medullary collecting duct (IMCD2 and IMCD3). In IMCD3, 5 min of stimulation by 2.5-250 nM AVP significantly depolymerized F-actin by 13-24% in whole cell assays employing the rhodamine-phalloidin binding technique. The IMCD2 was more sensitive, responding to subnanomolar (0.25 nM) AVP with 6 +/- 2% depolymerization. Depolymerization occurred as early as 2 min after 2.5 and 25 nM but not 250 nM AVP. 8-Bromoadenosine 3',5'-cyclic monophosphate depolymerized F-actin in IMCD3 at both 2 and 5 min. Immunogold labeling of the apical actin pool in IMCD3 principal cells was reduced by 26 +/- 5% (P < 0.05) by 2.5 nM AVP; the lateral and basal pools showed no significant changes. Capillary endothelial, thin limb of Henle, and intercalated cells showed no changes in immunogold labeling after AVP. Thus reorganization of the apical actin network by AVP is a consistent finding in both mammalian and amphibian target cells.

This article has been cited by other articles:

				P. Uawithya, T. Pisitkun, B. E. Ruttenberg, and M. A. Knepper Transcriptional profiling of native inner medullary collecting duct cells from rat kidney Physiol Genomics, January 17, 2008; 32(2): 229 - 253. [Abstract] [Full Text] [PDF]

				S. Shaw and D. Marples N-ethylmaleimide causes aquaporin-2 trafficking in the renal inner medullary collecting duct by direct activation of protein kinase A Am J Physiol Renal Physiol, April 1, 2005; 288(4): F832 - F839. [Abstract] [Full Text] [PDF]

				E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]

				D. Brown, T. Katsura, and C. E. Gustafson Cellular mechanisms of aquaporin trafficking Am J Physiol Renal Physiol, September 1, 1998; 275(3): F328 - F331. [Abstract] [Full Text] [PDF]

				K. Fushimi, S. Sasaki, and F. Marumo Phosphorylation of Serine 256 Is Required for cAMP-dependent Regulatory Exocytosis of the Aquaporin-2 Water Channel J. Biol. Chem., June 6, 1997; 272(23): 14800 - 14804. [Abstract] [Full Text] [PDF]

				E. Klussmann, G. Tamma, D. Lorenz, B. Wiesner, K. Maric, F. Hofmann, K. Aktories, G. Valenti, and W. Rosenthal An Inhibitory Role of Rho in the Vasopressin-mediated Translocation of Aquaporin-2 into Cell Membranes of Renal Principal Cells J. Biol. Chem., June 1, 2001; 276(23): 20451 - 20457. [Abstract] [Full Text] [PDF]

				G. Tamma, E. Klussmann, K. Maric, K. Aktories, M. Svelto, W. Rosenthal, and G. Valenti Rho inhibits cAMP-induced translocation of aquaporin-2 into the apical membrane of renal cells Am J Physiol Renal Physiol, December 1, 2001; 281(6): F1092 - F1101. [Abstract] [Full Text] [PDF]