AJP - Cell Physiology, Vol 265, Issue 2 C507-C513, Copyright © 1993 by American Physiological Society

ARTICLES

Sarcoplasmic reticulum Ca(2+)-ATPase and ryanodine receptor in cold-acclimated ducklings and thermogenesis

E. Dumonteil, H. Barre and G. Meissner
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill 27599-7260.

In birds, prolonged cold exposure induces the development of a nonshivering thermogenesis (NST) of muscular origin. NST is characterized by an increased heat production, which may be achieved by an increased ATP-dependent cycling of Ca2+ between the sarcoplasmic reticulum (SR) and cytosolic compartments in muscle. In this study, the effects of prolonged cold exposure on SR function were assessed by determining the contents of the SR Ca(2+)-ATPase and Ca2+ release channel (ryanodine receptor) in the gastrocnemius muscle of ducklings (Cairina moschata) kept at thermoneutrality (25 degrees C) or cold acclimated (4 degrees C, 5 wk). Measurement of oxalate-supported 45Ca2+ uptake by whole muscle homogenates revealed that the SR Ca(2+)-ATPase activity, and fraction of vesicles containing a ryanodine-sensitive Ca2+ release channel were increased by 30-50% in response to prolonged cold exposure. Sodium dodecyl sulfate-polyacrylamide gel and immunoblot analysis, 45Ca2+ uptake, Ca(2+)-ATPase activity and [3H]ryanodine binding measurements with unfractionated and "heavy" SR membrane fractions also indicated an elevated Ca(2+)-ATPase and Ca2+ release channel content in cold-acclimated ducklings. These results showed that the contents of two components directly involved in Ca2+ cycling by the SR are increased by cold acclimation, and we suggest that this is related to NST.

This article has been cited by other articles:

				D. C. F. da Costa and A. M. Landeira-Fernandez Thermogenic activity of the Ca2+-ATPase from blue marlin heater organ: regulation by KCl and temperature Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2009; 297(5): R1460 - R1468. [Abstract] [Full Text] [PDF]

				C. R. VIANNA, T. HAGEN, C.-Y. ZHANG, E. BACHMAN, O. BOSS, B. GEREBEN, A. S. MORISCOT, B. B. LOWELL, J. E. P. W. BICUDO, and A. C. BIANCO Cloning and functional characterization of an uncoupling protein homolog in hummingbirds Physiol Genomics, April 2, 2001; 5(3): 137 - 145. [Abstract] [Full Text] [PDF]

				F. Mitidieri and L. de Meis Ca2+ Release and Heat Production by the Endoplasmic Reticulum Ca2+-ATPase of Blood Platelets. EFFECT OF THE PLATELET ACTIVATING FACTOR J. Biol. Chem., October 1, 1999; 274(40): 28344 - 28350. [Abstract] [Full Text] [PDF]

				L. De Meis Control of heat production by the Ca2+-ATPase of rabbit and trout sarcoplasmic reticulum Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1738 - C1744. [Abstract] [Full Text] [PDF]

				F. Marmonier, C. Duchamp, F. Cohen-Adad, T. P. D. Eldershaw, and H. Barre Hormonal control of thermogenesis in perfused muscle of Muscovy ducklings Am J Physiol Regulatory Integrative Comp Physiol, November 1, 1997; 273(5): R1638 - R1648. [Abstract] [Full Text] [PDF]

				L. de Meis Uncoupled ATPase Activity and Heat Production by the Sarcoplasmic Reticulum Ca2+-ATPase. REGULATION BY ADP J. Biol. Chem., June 29, 2001; 276(27): 25078 - 25087. [Abstract] [Full Text] [PDF]