AJP - Cell Physiology, Vol 264, Issue 6 C1594-C1599, Copyright © 1993 by American Physiological Society

ARTICLES

Modulation of glucose metabolism in macrophages by products of nitric oxide synthase

J. E. Albina and B. Mastrofrancesco
Department of Surgery, Rhode Island Hospital, Providence.

Nitric oxide (NO) is a product of L-arginine metabolism that suppresses cellular oxidative metabolism through the inhibition of tricarboxylic acid cycle and electron transport chain enzymes. The impact of NO synthase (NOS) activity on specific pathways of glucose metabolism in freshly harvested and overnight-cultured rat resident peritoneal macrophages, at rest and after stimulation with zymosan, was investigated using radiolabeled glucose. NOS activity was modulated through the L-arginine concentration in culture media and the use of its specific inhibitor, NG-monomethyl-L-arginine, and quantitated using radiolabeled L-arginine. Results demonstrated that NOS activity was associated with increased glucose disappearance, glycolysis, and hexose monophosphate shunt activity and, in line with the known inhibition of oxidative metabolism associated with the production of NO, with a decrease in the flux of glucose and butyrate carbon through the tricarboxylic acid cycle. In addition, the relative increase in glucose utilization that follows zymosan stimulation was enhanced by treatments that suppressed NOS activity. These results demonstrate that the characteristics of glucose metabolism by macrophages are, to a significant extent, determined by products of NOS.

This article has been cited by other articles:

				M. Scharte, X. Han, D. J. Bertges, M. P. Fink, and R. L. Delude Cytokines induce HIF-1 DNA binding and the expression of HIF-1-dependent genes in cultured rat enterocytes Am J Physiol Gastrointest Liver Physiol, March 1, 2003; 284(3): G373 - G384. [Abstract] [Full Text] [PDF]

				E. Mahoney, J. Reichner, L. Robinson Bostom, B. Mastrofrancesco, W. Henry, and J. Albina Bacterial Colonization and the Expression of Inducible Nitric Oxide Synthase in Murine Wounds Am. J. Pathol., December 1, 2002; 161(6): 2143 - 2152. [Abstract] [Full Text] [PDF]

				S. C. Sampaio, M. C. C. Sousa-e-Silva, P. Borelli, R. Curi, and Y. Cury Crotalus durissus terrificus snake venom regulates macrophage metabolism and function J. Leukoc. Biol., October 1, 2001; 70(4): 551 - 558. [Abstract] [Full Text] [PDF]

				C. C. Nessel, W. L. Henry Jr., B. Mastrofrancesco, J. S. Reichner, and J. E. Albina Vestigial respiratory burst activity in wound macrophages Am J Physiol Regulatory Integrative Comp Physiol, June 1, 1999; 276(6): R1587 - R1594. [Abstract] [Full Text] [PDF]

				P. R. Miles, L. Bowman, A. Rengasamy, and L. Huffman Constitutive nitric oxide production by rat alveolar macrophages Am J Physiol Lung Cell Mol Physiol, March 1, 1998; 274(3): L360 - L368. [Abstract] [Full Text] [PDF]

				J. C. Zhuang and G. N. Wogan Growth and viability of macrophages continuously stimulated to produce nitric oxide PNAS, October 28, 1997; 94(22): 11875 - 11880. [Abstract] [Full Text] [PDF]