| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Cell Physiology, Vol 264, Issue 5 C1316-C1326, Copyright © 1993 by American Physiological Society
ARTICLES |
M. E. O'Donnell
Department of Human Physiology, School of Medicine, University of California, Davis 95616.
Vascular endothelial cells have been shown previously to possess a highly active Na-K-Cl cotransport system that mediates the major portion of total K influx and is regulated by a variety of vasoactive hormones and neurotransmitters. These observations suggest that the cotransporter may be an important component of endothelial cell function. The present study was conducted to investigate the role of Na-K-Cl cotransport in regulation of endothelial cell volume. Cultured bovine aortic endothelial cells were exposed to media of varying tonicities and Na-K-Cl cotransport activity assessed as bumetanide-sensitive K influx. Increasing the extracellular tonicity by increments as small as 10 mosM was found to cause significant stimulation of cotransport activity, and lowering tonicity reduced activity of the transporter. Exposure of endothelial cells to hypertonic medium was also found to increase bumetanide-sensitive net uptake of Na and K and total cellular Na and K content. Endothelial cell volume was evaluated by [14C]urea determination of intracellular water space in endothelial monolayers and by electronic cell sizing of suspended cells. Treatment of the cells with agents that stimulate Na-K-Cl cotransport activity was found to increase cell volume, whereas cotransport-inhibiting agents decreased cell volume. Exposure of the cells to hypertonic medium caused a rapid decrease in cell volume, followed by a regulatory volume increase that was greatly attenuated by bumetanide. The volume recovery was partially inhibited by the Na-H exchange inhibitor amiloride and was nearly abolished by bumetanide and amiloride in combination. Endothelial cells of pulmonary artery and cerebral microvessels were also found to exhibit increased Na-K-Cl cotransport activity on exposure to hypertonic media. These findings suggest that Na-K-Cl cotransport is of major importance in endothelial cell volume regulation.
This article has been cited by other articles:
|
|
 |
|
  J. Brillault, T. I. Lam, J. M. Rutkowsky, S. Foroutan, and M. E. O'Donnell Hypoxia effects on cell volume and ion uptake of cerebral microvascular endothelial cells Am J Physiol Cell Physiol, January 1, 2008; 294(1): C88 - C96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Chang, M. E. O'Donnell, and A. I. Barakat Shear stress and 17{beta}-estradiol modulate cerebral microvascular endothelial Na-K-Cl cotransporter and Na/H exchanger protein levels Am J Physiol Cell Physiol, January 1, 2008; 294(1): C363 - C371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S. Silva, A. Kapela, and N. M. Tsoukias A mathematical model of plasma membrane electrophysiology and calcium dynamics in vascular endothelial cells Am J Physiol Cell Physiol, July 1, 2007; 293(1): C277 - C293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Malek, C. Xu, E. S. Kim, and S. L. Alper Hypertonicity triggers RhoA-dependent assembly of myosin-containing striated polygonal actin networks in endothelial cells Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1645 - C1659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. F. Pedersen, M. E. O'Donnell, S. E. Anderson, and P. M. Cala Physiology and pathophysiology of Na+/H+ exchange and Na+-K+-2Cl- cotransport in the heart, brain, and blood Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R1 - R25. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Foroutan, J. Brillault, B. Forbush, and M. E. O'Donnell Moderate-to-severe ischemic conditions increase activity and phosphorylation of the cerebral microvascular endothelial cell Na+-K+-Cl- cotransporter Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1492 - C1501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. O'Donnell, V. Duong, J. Suvatne, S. Foroutan, and D. M. Johnson Arginine vasopressin stimulation of cerebral microvascular endothelial cell Na-K-Cl cotransporter activity is V1 receptor and [Ca] dependent Am J Physiol Cell Physiol, August 1, 2005; 289(2): C283 - C292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. G. Zani and H. G. Bohlen Sodium channels are required during in vivo sodium chloride hyperosmolarity to stimulate increase in intestinal endothelial nitric oxide production Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H89 - H95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. S. Baker, S. D. Cramer, M. Kennedy, D. G. Assimos, and R. P. Holmes Glycolate and glyoxylate metabolism in HepG2 cells Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1359 - C1365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lytle and T. McManus Coordinate modulation of Na-K-2Cl cotransport and K-Cl cotransport by cell volume and chloride Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1422 - C1431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Meyer, M. Flagella, R. L. Sutliff, J. N. Lorenz, M. L. Nieman, C. S. Weber, R. J. Paul, and G. E. Shull Decreased blood pressure and vascular smooth muscle tone in mice lacking basolateral Na+-K+-2Cl- cotransporter Am J Physiol Heart Circ Physiol, November 1, 2002; 283(5): H1846 - H1855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. D. Stamer, K. Peppel, M. E. O'Donnell, B. C. Roberts, F. Wu, and D. L. Epstein Expression of Aquaporin-1 in Human Trabecular Meshwork Cells: Role in Resting Cell Volume Invest. Ophthalmol. Vis. Sci., July 1, 2001; 42(8): 1803 - 1811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Suvatne, A. I. Barakat, and M. E. O'Donnell Flow-induced expression of endothelial Na-K-Cl cotransport: dependence on K+ and Cl{-} channels Am J Physiol Cell Physiol, January 1, 2001; 280(1): C216 - C227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-G. Petronini, R. R. Alfieri, M. N. Losio, A. E. Caccamo, A. Cavazzoni, M. A. Bonelli, A. F. Borghetti, and K. P. Wheeler Induction of BGT-1 and amino acid System A transport activities in endothelial cells exposed to hyperosmolarity Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2000; 279(5): R1580 - R1589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Russell Sodium-Potassium-Chloride Cotransport Physiol Rev, January 1, 2000; 80(1): 211 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. K. Putney, C. R. T. Vibat, and M. E. O'Donnell Intracellular Cl regulates Na-K-Cl cotransport activity in human trabecular meshwork cells Am J Physiol Cell Physiol, September 1, 1999; 277(3): C373 - C383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Al-Aswad, H. Gong, D. Lee, M. E. O’Donnell, J. D. Brandt, W. J. Ryan, A. Schroeder, and K. A. Erickson Effects of Na-K-2Cl Cotransport Regulators on Outflow Facility in Calf and Human Eyes In Vitro Invest. Ophthalmol. Vis. Sci., July 1, 1999; 40(8): 1695 - 1701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kawazu, K. Yamada, M. Nakamura, and A. Ota Characterization of Cyclosporin A Transport in Cultured Rabbit Corneal Epithelial Cells: P-Glycoprotein Transport Activity and Binding to Cyclophilin Invest. Ophthalmol. Vis. Sci., July 1, 1999; 40(8): 1738 - 1744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. C. O'Neill Physiological significance of volume-regulatory transporters Am J Physiol Cell Physiol, May 1, 1999; 276(5): C995 - C1011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Dall'Asta, O. Bussolati, R. Sala, A. Parolari, F. Alamanni, P. Biglioli, and G. C. Gazzola Amino acids are compatible osmolytes for volume recovery after hypertonic shrinkage in vascular endothelial cells Am J Physiol Cell Physiol, April 1, 1999; 276(4): C865 - C872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Maglova, W. E. Crowe, P. R. Smith, A. A. Altamirano, and J. M. Russell Na+-K+-Cl- cotransport in human fibroblasts is inhibited by cytomegalovirus infection Am J Physiol Cell Physiol, November 1, 1998; 275(5): C1330 - C1341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cai, L. Pestic-Dragovich, M. E. O'Donnell, N. Wang, D. Ingber, E. Elson, and P. De Lanerolle Regulation of cytoskeletal mechanics and cell growth by myosin light chain phosphorylation Am J Physiol Cell Physiol, November 1, 1998; 275(5): C1349 - C1356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sun and S. G. Murali Stimulation of Na+-K+-2Cl- cotransporter in neuronal cells by excitatory neurotransmitter glutamate Am J Physiol Cell Physiol, September 1, 1998; 275(3): C772 - C779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Matthews, J. A. Smith, E. C. Mun, and J. K. Sicklick Osmotic regulation of intestinal epithelial Na+-K+-Cl- cotransport: role of Cl- and F-actin Am J Physiol Cell Physiol, March 1, 1998; 274(3): C697 - C706. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
